Unlock instant, AI-driven research and patent intelligence for your innovation.

Strain Pandoraea sp. for removing ammonia nitrogen in sewage under low-temperature condition and isolated culture method

A low temperature, sewage technology, applied in microorganism-based methods, chemical instruments and methods, biological water/sewage treatment, etc. question

Active Publication Date: 2013-06-05
GUOZHONG AIHUA TIANJIN MUNICIPAL ENCIRONMENT ENG
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of temperature drop, the metabolic activity of mesophilic bacteria gradually decreases, and they lose their activity when they reach a certain temperature. However, the number of low-temperature microorganisms suitable for low-temperature environments cannot be reduced in number due to their own physiological characteristics and the inhibition of various ecological factors. Reaching the status of the dominant flora, resulting in a decline in the quality of effluent from sewage treatment plants in winter

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Strain Pandoraea sp. for removing ammonia nitrogen in sewage under low-temperature condition and isolated culture method
  • Strain Pandoraea sp. for removing ammonia nitrogen in sewage under low-temperature condition and isolated culture method
  • Strain Pandoraea sp. for removing ammonia nitrogen in sewage under low-temperature condition and isolated culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for isolating and cultivating bacteria Pandoraea sp. that removes ammonia nitrogen in sewage under low temperature conditions, comprising the following steps:

[0025] (1) the first kind of nitrous bacteria culture solution is put into the small test SBR reactor, the sludge inoculated in the first kind of nitrite bacteria culture solution with the aeration tank of Tianjin Jizhuangzi Sewage Treatment Plant, at 10 DEG C , continuous culture for 5 days, take out the supernatant, add the second nitrous acid bacteria culture solution, at 10 ℃, continue to cultivate for 5 days, take out the supernatant, add the third nitrous acid bacteria culture solution, at 10 ℃, continue to cultivate Cultivate for 5 days to enrich and domesticate low-temperature bacteria;

[0026] (2) Take 10mL of the sludge obtained in step (1), add it to a 250ml Erlenmeyer flask filled with 90mL sterilized distilled water and 30 sterilized glass beads, and shake for 20min to fully release the ba...

Embodiment 2

[0030] The first nitrous bacteria culture solution is composed of the following components: NH 4 Cl 0.0764g, sodium acetate 2g, MgSO 4 7H 2 O0.05g, K 2 HPO 4 0.2g, NaCl 0.12g, MnSO 4 h 2 O 0.01g, FeSO 4 0.01g, add distilled water to 1000mL.

Embodiment 3

[0032] The second nitrous bacteria culture solution is composed of the following components: NH 4 Cl 0.191g, sodium acetate 2g, MgSO 4 7H 2 O 0.05g, K 2 HPO 4 0.2g, NaCl 0.12g, MnSO 4 h 2 O 0.01g, FeSO 4 0.01g, add distilled water to 1000mL.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a strain Pandoraea sp. for removing ammonia nitrogen in sewage under a low-temperature condition and an isolated culture method. The strain Pandoraea sp.-2CGMCC No. 5284 for removing the ammonia nitrogen in the sewage under the low-temperature condition has the capacity of removing the ammonia nitrogen in the sewage under the low-temperature aerobic condition. Experimentalresults show that: although the strain is obviously inhibited by a low-temperature environment, the strain Pandoraea sp.-2CGMCC No. 5284 for removing the ammonia nitrogen in the sewage under the low-temperature condition has an obvious reinforcing effect of removing the ammonia nitrogen at the low temperature of 10 DEG C, the removal rate of the ammonia nitrogen is improved by 10 percent, and thehighest concentration of the effluent ammonia nitrogen is 5.1mg / L and superior to the effluent standard of the first grade A (when the water temperature is less than or equal to 12 DEG C, the controlindex of the first grade A standard is 8mg / L).

Description

technical field [0001] The invention belongs to the field of sewage treatment, and relates to a bacterium Pandoraea sp. for removing ammonia nitrogen in sewage under low temperature conditions and an isolation and cultivation method. Background technique [0002] The reason why low temperature has a significant impact on biological treatment capacity is that the decrease in temperature affects the growth and metabolism of microorganisms and changes the microbial flora in sewage treatment systems. In the process of temperature drop, the metabolic activity of mesophilic bacteria gradually decreases, and they lose their activity when they reach a certain temperature. However, the number of low-temperature microorganisms suitable for low-temperature environments cannot be reduced in number due to their own physiological characteristics and the inhibition of various ecological factors. Reaching the status of the dominant flora, which leads to a decline in the quality of the efflu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/01
CPCY02W10/10
Inventor 胡春萍杨蛟云刘智晓熊欢欢张伟沈旺
Owner GUOZHONG AIHUA TIANJIN MUNICIPAL ENCIRONMENT ENG
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More