Strain Pandoraea sp. for removing ammonia nitrogen in sewage under low-temperature condition and isolated culture method
A low temperature, sewage technology, applied in microorganism-based methods, chemical instruments and methods, biological water/sewage treatment, etc. question
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Embodiment 1
[0024] A method for isolating and cultivating bacteria Pandoraea sp. that removes ammonia nitrogen in sewage under low temperature conditions, comprising the following steps:
[0025] (1) the first kind of nitrous bacteria culture solution is put into the small test SBR reactor, the sludge inoculated in the first kind of nitrite bacteria culture solution with the aeration tank of Tianjin Jizhuangzi Sewage Treatment Plant, at 10 DEG C , continuous culture for 5 days, take out the supernatant, add the second nitrous acid bacteria culture solution, at 10 ℃, continue to cultivate for 5 days, take out the supernatant, add the third nitrous acid bacteria culture solution, at 10 ℃, continue to cultivate Cultivate for 5 days to enrich and domesticate low-temperature bacteria;
[0026] (2) Take 10mL of the sludge obtained in step (1), add it to a 250ml Erlenmeyer flask filled with 90mL sterilized distilled water and 30 sterilized glass beads, and shake for 20min to fully release the ba...
Embodiment 2
[0030] The first nitrous bacteria culture solution is composed of the following components: NH 4 Cl 0.0764g, sodium acetate 2g, MgSO 4 7H 2 O0.05g, K 2 HPO 4 0.2g, NaCl 0.12g, MnSO 4 h 2 O 0.01g, FeSO 4 0.01g, add distilled water to 1000mL.
Embodiment 3
[0032] The second nitrous bacteria culture solution is composed of the following components: NH 4 Cl 0.191g, sodium acetate 2g, MgSO 4 7H 2 O 0.05g, K 2 HPO 4 0.2g, NaCl 0.12g, MnSO 4 h 2 O 0.01g, FeSO 4 0.01g, add distilled water to 1000mL.
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