Fermentation method for improving quinoxalone yield
A quinacomycin and fermentation method technology, applied in the field of fermentation technology, can solve the problems of difficult product extraction and separation, metabolite repression, low yield and purity of quinacomycin, etc., and achieve high yield and purity, stable yield, and reduced process cost effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] (1) Inclined solid culture
[0029] Inoculate Stigmatella erecta LY-122 on a solid slant medium and culture at 30°C for 5 days to obtain the strains cultured on the slant solid, and set aside;
[0030] The formula of the solid slant medium is: for every 1000 ml medium, add 3.0 g of peptone, 3.0 g of glucose, 17.0 g of agar, and the balance is water;
[0031] (2) Preparation of shake flask seed solution
[0032] Take 4 rings of strains and add 16ml of sterile water to make a bacterial suspension from the strains prepared on the slant solid culture prepared in the above step (1), and inoculate the prepared bacterial suspensions into 10 cultured In the liquid seed medium in a 150 ml shake flask based on the base, the inoculation amount was 5% of the volume of the liquid seed medium, the culture temperature was 30°C, the stirring speed was 200 r / min, the pH was 7.1, and the culture time was 5 days to obtain the shake flask seed liquid ,spare;
[0033] The formula of the ...
Embodiment 2
[0041] (1) Inclined solid culture
[0042] Inoculate Stigmatella erecta LY-122 on a solid slant medium, and culture it at 25°C for 7 days to obtain the strains cultured on the slant solid, and set aside;
[0043] The formula of the solid slant medium is: for every 1000 ml medium, add 1.0 g of peptone, 1.0 g of glucose, 17.0 g of agar, and the balance is water;
[0044] (2) Preparation of shake flask seed solution
[0045] For the bacteria cultured on the slant prepared in the above step (1), take a ring of the bacteria and add 3ml of sterile water to make a bacterial suspension, and inoculate the prepared bacterial suspension into four cultured cells containing 30 ml of liquid seeds respectively. In the liquid seed medium in a 150 ml shake flask based on the base, the inoculation amount was 2% of the volume of the liquid seed medium, the culture temperature was 25°C, the stirring speed was 150 r / min, the pH was 7.0, and the culture time was 5 days to obtain the shake flas...
Embodiment 3
[0054] (1) Inclined solid culture
[0055] Inoculate Stigmatella erecta LY-122 on a solid slant medium, and culture it at 25°C for 7 days to obtain the strains cultured on the slant solid, and set aside;
[0056] The formula of the solid slant medium is: for every 1000 ml medium, add 5.0 g of peptone, 5.0 g of glucose, 17.0 g of agar, and the balance is water;
[0057] (2) Preparation of shake flask seed solution
[0058] For the bacteria cultured on the slant prepared in the above step (1), take 12 rings of bacteria and add 60ml of sterile water to make a bacterial suspension, and inoculate the prepared bacterial suspension in 20 cells containing 30 ml of liquid seed culture In the liquid seed medium in a 150 ml shake flask based on the base, the inoculation amount was 10% of the volume of the liquid seed medium, the culture temperature was 35°C, the stirring speed was 200 r / min, the pH was 7.0, and the culture time was 2 days to obtain the shake flask seed liquid ,spare; ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| purity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More