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Gene chip for detecting Angelman syndromes, using method and kit for gene chip

A gene chip and syndrome technology, which is applied in the field of gene chip detection of Angelman syndrome and its use and kit, to achieve the effects of good stability, high detection specificity and high repeatability

Active Publication Date: 2012-07-11
SHANGHAI BIOGERM MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no rapid, low-cost, high-throughput and automated method for detecting gene mutations in Angelman syndrome

Method used

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  • Gene chip for detecting Angelman syndromes, using method and kit for gene chip
  • Gene chip for detecting Angelman syndromes, using method and kit for gene chip
  • Gene chip for detecting Angelman syndromes, using method and kit for gene chip

Examples

Experimental program
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Effect test

Embodiment

[0021] The gene chip of this embodiment consists of a solid phase carrier and an oligonucleotide probe immobilized on the solid phase carrier, and the oligonucleotide probe is a nucleotide sequence shown in SEQ ID NO.1-50, The solid phase carrier is glass slide. Nitrocellulose membrane, silicon wafer, nylon membrane or polystyrene can also be used for the solid-phase loading of the gene chip in this embodiment. The oligonucleotide probes in this example were produced by Agilent, and the gene chip in this example was also produced by Agilent according to conventional methods.

[0022] The method for using the gene chip in this embodiment, the steps are as follows:

[0023] (1) Preparation of sample DNA fragments

[0024] The blood of patients and normal people was taken, and the whole genome DNA (gDNA) was extracted by conventional methods.

[0025] Adjust the temperature of the water bath to 37°C and 65°C, dissolve 10×Buffer C (see the table below), vortex briefly and then ...

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Abstract

The invention relates to a gene chip for detecting Angelman syndromes, and a using method and a kit for the gene chip. The gene chip comprises a solid-phase carrier and a probe fixed to the solid-phase carrier, wherein the oligonucleotide probe is a nucleotide sequence shown as SEQ ID NO. 1-50. The using method comprises the following steps of: (a) preparing a deoxyribonucleic acid (DNA) fragment of a sample; (b) marking the DNA fragment by using fluorescence; (c) eluting the marked product; (d) hybridizing the marked product and the gene chip; and (e) scanning and detecting a hybridization signal of the gene chip to obtain a result. The invention also relates to the kit for the gene chip. The gene chip has simple operating steps and is high in detection specificity and stability and low in cost, and the scanned result extracted from the sample can be completed within one working day, so the gene chip is low in cost and suitable for the genetic mutation detection of clinical patients, prenatal diagnosis and the detection of normal human heterozygote carriers.

Description

technical field [0001] The invention relates to a gene chip in the field of biotechnology, a use method, and a kit, in particular to a gene chip for detecting Angelman syndrome, a use method, and a kit. Background technique [0002] Angelman syndrome (Angelman syndrome, AS), also known as happy puppet syndrome, is a genome imprinting disease, which was first reported by Hurry Angelman in 1965. Clinically, the disease manifests as a complex multisystem abnormality. The main clinical symptoms include microcephaly, happy puppet-like ataxic gait, seizures, EEG abnormalities, hyperexcitability, and episodic laughter. Abnormalities in the q11-13 region of maternal chromosome 15 are the main cause of the disease, and four molecular pathological mechanisms have been identified that can lead to the occurrence of AS. The incidence of AS reported abroad is about 2.5 / 100000-10 / 100000. The current research shows that four clear mechanisms can lead to the occurrence of Angleman syndrome...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 贺林李笑天秦胜营郭奇桑吴茜龚小会
Owner SHANGHAI BIOGERM MEDICAL TECH CO LTD
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