Preparation method and application of enzyme regulated nanometer supermolecule vesicle

Abstract

The invention discloses enzyme regulated nanometer supermolecule vesicle of which the building unit takes C4AS as a host and MYC1 as a guest, and a supramolecule assembly is built through inclusion complexation and mutual effect of the host and the gust; and the preparation method is as follows: C4AS and MYC1 are dissolved into water, and supermolecule vesicle solution is obtained after uniform mixing, and choline esterase is added so as to enable supermolecule vesicle in supermolecule vesicle solution to depolymerize gradually by the time. The invention has the advantages as follows: the preparation method is simple and convenient, and the use level of host and guest raw materials are less; the supermolecule vesicle is biocompatible and have excellent stability, has an excellent select responsiveness to choline esterase, can load hydrophilic drug tacrine for curing senile dementia, the loaded tacrine has high selectivity and is released on the target position of the choline esterase properly, and the enzyme regulated nanometer supermolecule vesicle has a wide application scope in the field of loading, transporting and target releasing drug for curing senile dementia.

Description

【Technical field】 [0001] The invention belongs to the technical field of nano-supramolecular materials, in particular to a preparation method and application of enzyme-regulated nano-supramolecular vesicles. 【Background technique】 [0002] Stimuli-responsive vesicles have received extensive attention in recent years because of their many important applications in the fields of chemistry, biology and material science (A. Mueller, D.F.O'Brien. Chem. Rev. 2002, 102, 727-757; ( 2) D.M. Vriezema, M.C. Aragones, J.A.A.W. Elemans, J.J.L.M. Cornelissen, A.E. Rowan, R.J.M. Nolte. Chem. Rev. 2005, 105, 1445-1489; (3) X. Guo, F. C. Szoka. 36, 335-341.). The drug / gene delivery system of stimuli-responsive vesicles has become a research hotspot because it can release the cargo at a specific site, because it can not only improve the drug efficacy of the cargo, but also reduce its toxic side effects. Most of the currently reported stimuli-responsive vesicles are systems that respond to s...

AI Technical Summary

Problems solved by technology

[0003] However, the introduction of enzyme-responsive sites usually requires complex and time-consuming covalent bond synthesis, which not only increases the cost of preparation, but also introduces organic solvents and toxic reagents into the vesicle system during the synthesis process, thereby reducing its biological efficiency. compatibility

Images