Method for simultaneously separating dihydromyricetin and myricetin from ampelopsis plant

Abstract

The invention discloses a method for simultaneously separating dihydromyricetin and myricetin from an ampelopsis plant. The method comprises the following steps of: drying a sample, roughly smashing to 20-40 meshes, extracting with boiling water twice, combining extracting solutions, performing suction filtration in a hot state, standing a filtrate at the temperature of 0-4 DEG C for separating a white precipitate out, performing suction filtration or centrifuging, dissolving the filtrated precipitate with absolute ethyl alcohol, standing, performing suction filtration, concentrating a filtrate under reduced pressure to obtain a thick solution, evaporating ethanol to dryness, and drying at a controlled temperature to obtain general flavone; performing reflux extraction on the general flavone with acetone, concentrating an extracting solution to obtain crude dihydromyricetin, performing reflux extraction on insoluble residues by using ethanol and water in the ratio of 1:1, and concentrating an extracting solution to obtain crude myricetin 1; and during recrystallization and purification of dihydromyricetin with water (recrystallization times are more than three), filtering by heating (to 85-95 DEG C) to obtain a yellow transparent solution serving as a filtrate, collecting a large quantity of yellow green substances (myricetin 2) on filter paper, and drying to obtain myricetin 2. According to HPLC (High Performance Liquid Chromatography) detection, the purity of myricetin 2 is 87.37 percent, and the purity of dihydromyricetin (while needle crystal) is 89.87 percent.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of chemical components of plants of the family Vitis genus, and can be used for the simultaneous separation of myricetin and dihydromyricetin in stems and leaves of plants of the genus Snakevine and its processed product, vine tea. Background technique [0002] Plants of the genus Vitis vinifera contain flavonoid components myricetin and dihydromyricetin, among which Vitis dentata ( Ampelopsis-grossedentada ) content is the highest, and the processed product of Snake-tooth grape stems and leaves is commonly known as vine tea, vine tea has a variety of physiological activities, the main active ingredients are flavonoids, and the content of dihydromyricetin is the highest, and the main active ingredient flavonoids content can be as high as More than 40%. In addition, it also contains myricetin, catechin, gallic acid, amino acids, trace elements and other ingredients; the main comp...

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problem that myricetin and dihydromyricetin are difficult to separate and is difficult to apply to industrialization, and proposes a method for simultaneously separating dihydromyricetin and myricetin from plants of the genus Ophiocarpus