Method for simultaneously separating dihydromyricetin and myricetin from ampelopsis plant

A technology for dihydromyricetin and purification of dihydromyricetin, which is applied in the direction of organic chemistry and the like, can solve the problems of difficult separation and industrialization of myricetin and dihydromyricetin, and achieves simple equipment, simple operation and high product purity. Effect

Inactive Publication Date: 2012-07-18
郁建平
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The present invention aims to solve the problem that myricetin and dihydromyricetin are difficult to separate and is difficult to apply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 2

[0027] 1. Guangdong Snake Grape ( Amplelopsis cantoniensis Extraction and refinement of dihydromyricetin and myricetin in Planch

[0028] Weigh 100g of dried and coarsely crushed Guangdong snake grape stem and leaf samples, extract in two boiling water, add 1000mL of water for the first time, heat and boil for 5-10 minutes, pour out the decoction, add 500-1000mL of water, heat and boil for 5-10 minutes Minutes, combine the extracts, and suction filter while it is hot. The filtrate is allowed to stand at 0°C~4°C to precipitate a white precipitate, filter with suction, dissolve the filtered precipitate with 100mL of absolute ethanol, stand for suction filtration, and concentrate the filtrate under reduced pressure until the solution is thick. Pour out the thick liquid, evaporate the ethanol to get yellow lumps (total flavonoids), dry at 50°C~65°C, grind it finely with a mortar after drying, weigh it, and pour it into a paper tube for separation .

[0029] 2. Dihydromyricetin...

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Abstract

The invention discloses a method for simultaneously separating dihydromyricetin and myricetin from an ampelopsis plant. The method comprises the following steps of: drying a sample, roughly smashing to 20-40 meshes, extracting with boiling water twice, combining extracting solutions, performing suction filtration in a hot state, standing a filtrate at the temperature of 0-4 DEG C for separating a white precipitate out, performing suction filtration or centrifuging, dissolving the filtrated precipitate with absolute ethyl alcohol, standing, performing suction filtration, concentrating a filtrate under reduced pressure to obtain a thick solution, evaporating ethanol to dryness, and drying at a controlled temperature to obtain general flavone; performing reflux extraction on the general flavone with acetone, concentrating an extracting solution to obtain crude dihydromyricetin, performing reflux extraction on insoluble residues by using ethanol and water in the ratio of 1:1, and concentrating an extracting solution to obtain crude myricetin 1; and during recrystallization and purification of dihydromyricetin with water (recrystallization times are more than three), filtering by heating (to 85-95 DEG C) to obtain a yellow transparent solution serving as a filtrate, collecting a large quantity of yellow green substances (myricetin 2) on filter paper, and drying to obtain myricetin 2. According to HPLC (High Performance Liquid Chromatography) detection, the purity of myricetin 2 is 87.37 percent, and the purity of dihydromyricetin (while needle crystal) is 89.87 percent.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of chemical components of plants of the family Vitis genus, and can be used for the simultaneous separation of myricetin and dihydromyricetin in stems and leaves of plants of the genus Snakevine and its processed product, vine tea. Background technique [0002] Plants of the genus Vitis vinifera contain flavonoid components myricetin and dihydromyricetin, among which Vitis dentata ( Ampelopsis-grossedentada ) content is the highest, and the processed product of Snake-tooth grape stems and leaves is commonly known as vine tea, vine tea has a variety of physiological activities, the main active ingredients are flavonoids, and the content of dihydromyricetin is the highest, and the main active ingredient flavonoids content can be as high as More than 40%. In addition, it also contains myricetin, catechin, gallic acid, amino acids, trace elements and other ingredients; the main comp...

Claims

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Application Information

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IPC IPC(8): C07D311/32C07D311/30C07D311/40
Inventor 郁建平伍贵祥范家佑郁浩翔樊静静
Owner 郁建平
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