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Quality detection method for unfertilized egg of turbo

An unfertilized egg and detection method technology, applied in the field of quality inspection of turbot unfertilized eggs, can solve the problems of inability to give experimental result data in the shortest time, low accuracy, time-consuming and labor-intensive, etc., and achieve perfection. The evaluation system of the eggs of teleost and the effect of improving production efficiency

Active Publication Date: 2012-07-18
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

Although the detection from the appearance is fast, the accuracy is low; while the detection through the hatching rate has the highest accuracy, but it is time-consuming and labor-intensive, and the experimental results cannot be given in the shortest time.

Method used

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  • Quality detection method for unfertilized egg of turbo
  • Quality detection method for unfertilized egg of turbo
  • Quality detection method for unfertilized egg of turbo

Examples

Experimental program
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Embodiment Construction

[0016] Below in conjunction with embodiment the method of the present invention is described in detail:

[0017] 1) Determine the amount of eggs:

[0018] Take 1ml, 3ml, 5ml, 10ml of the mixture of turbot ovum and ovary fluid respectively with a straw, place them in the grids of different sizes on the top plate of the portable turbot ovum rapid counting board, and then calculate the ovum and ovary fluid per milliliter. The number of eggs in the mixture of fluid, and finally the number of eggs is obtained according to the volume of the mixture of eggs and ovarian fluid;

[0019] The counting plate can be made of materials such as plastics, for example, the material used to prepare a 96-well cell culture plate can also be used to prepare the counting plate of the present invention. In appearance, the counting plate is similar to the cell culture plate currently used (such as figure 1 ), including a substrate 1 as a carrier, and a base 4 for containing the substrate 1 , the bas...

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Abstract

The invention relates to a quality detection method for an unfertilized egg of turbot. When the RNA (Ribose Nucleic Acid) concentration of the unfertilized egg of the turbot is 780+ / -0.001mu g / egg and the ratio of Pr to RNA to DNA (Deoxyribose Nucleic Acid) is (227-250) to (12-13.5) to (1-1.2), the fertility rate and the hatchability are highest and the quality of the egg is optimal at the same time. According to the quality detection method disclosed by the invention, the quality of turbot eggs can be quickly and effectively detected, thereby quickly determining whether the eggs to be researched meet the requirement demand and increasing the production efficiency; and meanwhile, the quality evaluation system of teleostean eggs is further completed, and an effective platform and an reference are provided for the quality evaluation for researching other cultivated seawater fish eggs.

Description

technical field [0001] The invention belongs to the technical field of egg quality detection, in particular to a quality detection method of turbot unfertilized eggs. Background technique [0002] Turbot (Scophthalmus maximus) is one of the most important seawater cultured fishes in Europe. Since turbot was introduced to China in 1992, with the joint efforts of aquaculture researchers and breeding enterprises, turbot has been widely used in the field of industrial aquaculture. Significant results have been achieved, with the highest annual output reaching more than 50,000 tons and an annual output value of more than 4 billion yuan, thus becoming a new economic growth point for my country's aquaculture industry. [0003] In the process of artificial breeding of turbot and other bony fishes, the quality of eggs is a key factor affecting reproductive performance. It is directly related to the development of embryos and the survival and growth of larvae, juveniles, and juveniles,...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12Q1/68G01N21/33
Inventor 贾玉东孟振刘新富高淳仁雷霁霖
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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