Method for artificially inducing field wheat scab

A technology for scab and scab fungus, which is applied to the field of artificial inoculation inducing wheat scab in the field, can solve the problems of high cost of spray moisturizing facilities, labor costs, etc., so as to improve the induction effect of artificial inoculation, and improve the environmental humidity and temperature. balanced effect

Inactive Publication Date: 2012-07-25
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to propose a simple, labor-saving, low-cost and highly effective method for inducing head blight in view of the shortcomings of the traditional "soil surface inoculation met...

Method used

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  • Method for artificially inducing field wheat scab
  • Method for artificially inducing field wheat scab
  • Method for artificially inducing field wheat scab

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: (the method for artificially inducing field wheat scab)

[0023] The method proceeds as follows:

[0024] (1) Cultivation of Gibberella zeae (Schw.) Petch conidia: select Gibberella zeae (Schw.) Petch strain ACCC31055 as the strain; cultivate and activate the strain on potato medium, then transfer to mung bean soup for cultivation After vibrating culture at 28°C and 280 rev / min for 7 days and filtering with gauze to obtain the filtrate, add sterile water to the liquid and observe under a microscope until the content of conidia in the liquid is 10 7 pcs / ml, reserve; among them,

[0025] The potato medium formula is: 200 grams of potatoes, 20 grams of sucrose, 17 grams of agar and 1000 ml of water; the preparation method is: wash the potatoes, peel them, shred them, add 500 ml of water and boil for 15 minutes, filter to obtain potato juice Add agar to 500ml of water, cook until the agar dissolves, filter to obtain agar solution; mix potato juice and agar s...

Embodiment 2

[0038] Embodiment 2: (the method for artificially inducing field barley scab)

[0039] In this example, in step (2), the filtrate of step (1), 5% water-retaining agent polyshuibao, 10% surfactant XB804 and sterile water are used in a ratio of 10% by weight: 5%: 5%: 80%. mixed to produce conidia with a content of 10 6 Individual / ml, polyhydric retention content 0.25%, XB804 content 0.5% scab inoculum of wheat class; The used barley variety of step (4) is No. 3 of Zhepi; Step (5) investigation time is 25 days after inoculation, The incidence rate of head blight in barley Zhepi No. 3 was 92.5%, and the average reaction grade was 3.28. All the other steps are the same as in Example 1.

Embodiment 3

[0040] Embodiment 3: (the method for artificially inducing field wheat scab)

[0041] In this example, in step (2), the filtrate of step (1), 5% water-retaining agent polyshuibao, 10% surfactant XB804 and sterile water are 0.01% by weight: 0.1%: 10%: 89.89% mixed to produce conidia with a content of 10 4 The inoculum of wheat class Fusarium rubella of individual / ml, polywater retention content 0.05%, XB804 content 0.025%; The wheat variety used in step (4) is Yangmai 158; The investigation time of step (5) is 22 days after inoculation, barley The incidence rate of scab in Zhepi 3 was 95%, and the average reaction grade was 2.76. All the other steps are the same as in Example 1.

[0042] In order to clarify the induction effect of the method of the present invention for artificially inducing wheat head blight in the field, the applicant conducted the following experiments.

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Abstract

The invention discloses a method for artificially inducing field wheat scab, belonging to plant disease technical field. The method includes culturing conidiophore of wheat scab strain; preparing wheat scab strain inoculum; preparing moisture-retaining cloth; inoculating wheat scab strain inoculum, and retaining moisture; observing disease condition, recording, and performing statistics. The method retains moisture with moisture-retaining cloth, to significantly improve environmental humidity of wheat ear position and balance temperature, thereby facilitating invasion and fixation of wheat scab strain and improving field wheat scab artificial inducement effect. The method has simple implementation, no need of high input of manual labor and high-cost facilities, and applicability to wheat planting test areas.

Description

technical field [0001] The invention relates to the technical field of plant diseases, in particular to a simple, efficient and labor-saving method for inducing wheat head blight by artificial inoculation in the field. Background technique [0002] Wheat head blight is one of the main diseases in the warm, humid and semi-humid wheat regions of the world, and it is also an important disease that affects the yield and quality of wheat in the middle and lower reaches of the Yangtze River and South my country wheat regions. It includes barley scab and wheat scab, the causative bacteria of these two diseases are Gibberella zeae (Schw.) Petch, and the yield loss caused by them is generally 10% to 20% in a year, The reissue year can reach 20% to 30%. [0003] The occurrence of wheat head blight needs to meet the following two conditions: one is the source of pathogenic bacteria, and the other is suitable temperature and humidity conditions. If these two conditions are not met, it...

Claims

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Application Information

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IPC IPC(8): C12N3/00A01G7/06C12R1/645
Inventor 王连平陶荣祥郝中娜梁建根
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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