Protein A immunosorbent material and preparation method thereof

Abstract

The invention discloses a protein A immunosorbent material for adsorbing an antibody, and the protein A immunosorbent material is a polymer material coupled by agarose gel and protein A, wherein the protein A is a recombinant protein A with cysteine. The protein A immunosorbent material is prepared through the following three steps: preparing the recombinant protein A, activating the agarose gel, and synthesizing the immunosorbent material. The protein A immunosorbent material can adsorb the pathogenic antibody related to immunological diseases of the material from body fluid directly, efficiently and selectively, can safely perform extracorporeal circulation, and realizes once one-off perfusion to achieve the aim of removing the pathogenic antibody of a patient.

Description

technical field [0001] The invention relates to the field of medical biological materials, in particular to a protein A immunoadsorption material used for adsorbing antibodies and a preparation method thereof. Background technique [0002] Even in today's medically developed world, there are still many difficult diseases that humans cannot overcome, and autoimmune diseases are one of them. The pathogenesis of autoimmune diseases is complex and can involve multiple organs and systems of the whole body. The traditional treatment methods are mainly glucocorticoids and cytotoxic drugs, and the treatment effect has been poor. Autoimmune diseases have become one of the major diseases that threaten human health and life. [0003] Autoimmune diseases are characterized by the detection of one or more high titers of autoantibodies in the serum or other body fluids of patients. Autoantibodies are an important basis for the diagnosis of autoimmune diseases. Each autoimmune disease is a...

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Problems solved by technology

[0006] At present, there are many patents on the synthesis method of protein A immunoadsorbent materials. Generally, the primary amino group on the molecular chain of protein A reacts with other groups to immobilize on the agarose gel matrix, because the chemical reactivity of the primary amino group is not high. , published literature and patent experimental data show that after the primary amino group on protein A reacts with the carrier, the content of protein A immobilized on the carrier is 3-10 mg / mL carrier, and the synthesized material adsorbs 10-25 mg antibody per ml , because the adsorption capacity of the adsorption column for antibodies is not high, and one-time perfusion cannot achieve the purpose of removing pathogenic antibodies, the adsorption column must be equipped with CITEM 10 system, which hinders the wide application of protein A immunoadsorption therapy in clinical practice

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