Protein A immunosorbent material and preparation method thereof

An immunoadsorption material and protein technology, which is applied in the field of protein A immunoadsorption materials and its preparation, can solve the problems of low adsorption capacity, low chemical reactivity of primary amine groups, and obstacles to the wide application of protein A immunoadsorption therapy. The effect of safe extracorporeal circulation and clearing patients' pathogenic antibodies

Inactive Publication Date: 2012-08-01
李树兴
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are many patents on the synthesis method of protein A immunoadsorbent materials. Generally, the primary amino group on the molecular chain of protein A reacts with other groups to immobilize on the agarose gel matrix, because the chemical reactivity of the primary amino group is not high. , published literature and patent experimental data show that after the primary amino group on protein A reacts with the carrier, the co...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein A immunosorbent material and preparation method thereof
  • Protein A immunosorbent material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1), the preparation of recombinant protein A: the coding sequence (SEQ ID NO: 1) of protein A with cysteine ​​is obtained by artificial synthesis, the sequence comprises the sequence of the EDABC domain in the natural protein A, a The codon tgt encoding cysteine, the recognition sequence of restriction endonuclease NdeI at the 5' end of the sequence and the recognition sequence of XhoI at the 3' end of the sequence, respectively.

[0024] SEQ ID NO: 1

[0025] gggcatatggcgcaacacgatgaagctcaacaaaatgctttttatcaagtcttaaatatgcctaacttaaatgctgatcaacgcaatggttttatccaaagccttaaagatgatccaagccaaagtgctaacgttttaggtgaagctcaaaaacttaatgactctcaagctccaaaaGctgatgcgcaacaaaataacttcaacaaagatcaacaaagcgccttctatgaaatcttgaacatgcctaacttaaacgaagcgcaacgtaacggcttcattcaaagtcttaaagacgacccaagccaaagcactaacgttttaggtgaagctaaaaaattaaacgaatctcaagcaccgaaaGctgataacaatttcaacaaagaacaacaaaatgctttctatgaaatcttgaatatgcctaacttaaacgaagaacaacgcaatggtttcatccaaagcttaaaagatgacccaagccaaagtgctaacctattgtcagaagctaaaaagttaaatgaa...

Embodiment 2

[0031] (1), the preparation of recombinant protein A: the coding sequence (SEQ ID NO: 1) of protein A with cysteine ​​is obtained by artificial synthesis, the sequence comprises the sequence of the EDABC domain in the natural protein A, a The codon tgt encoding cysteine, the recognition sequence of restriction endonuclease NdeI at the 5' end of the sequence and the recognition sequence of XhoI at the 3' end of the sequence, respectively.

[0032] SEQ ID NO: 1

[0033] gggcatatggcgcaacacgatgaagctcaacaaaatgctttttatcaagtcttaaatatgcctaacttaaatgctgatcaacgcaatggttttatccaaagccttaaagatgatccaagccaaagtgctaacgttttaggtgaagctcaaaaacttaatgactctcaagctccaaaaGctgatgcgcaacaaaataacttcaacaaagatcaacaaagcgccttctatgaaatcttgaacatgcctaacttaaacgaagcgcaacgtaacggcttcattcaaagtcttaaagacgacccaagccaaagcactaacgttttaggtgaagctaaaaaattaaacgaatctcaagcaccgaaaGctgataacaatttcaacaaagaacaacaaaatgctttctatgaaatcttgaatatgcctaacttaaacgaagaacaacgcaatggtttcatccaaagcttaaaagatgacccaagccaaagtgctaacctattgtcagaagctaaaaagttaaatgaa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a protein A immunosorbent material for adsorbing an antibody, and the protein A immunosorbent material is a polymer material coupled by agarose gel and protein A, wherein the protein A is a recombinant protein A with cysteine. The protein A immunosorbent material is prepared through the following three steps: preparing the recombinant protein A, activating the agarose gel, and synthesizing the immunosorbent material. The protein A immunosorbent material can adsorb the pathogenic antibody related to immunological diseases of the material from body fluid directly, efficiently and selectively, can safely perform extracorporeal circulation, and realizes once one-off perfusion to achieve the aim of removing the pathogenic antibody of a patient.

Description

technical field [0001] The invention relates to the field of medical biological materials, in particular to a protein A immunoadsorption material used for adsorbing antibodies and a preparation method thereof. Background technique [0002] Even in today's medically developed world, there are still many difficult diseases that humans cannot overcome, and autoimmune diseases are one of them. The pathogenesis of autoimmune diseases is complex and can involve multiple organs and systems of the whole body. The traditional treatment methods are mainly glucocorticoids and cytotoxic drugs, and the treatment effect has been poor. Autoimmune diseases have become one of the major diseases that threaten human health and life. [0003] Autoimmune diseases are characterized by the detection of one or more high titers of autoantibodies in the serum or other body fluids of patients. Autoantibodies are an important basis for the diagnosis of autoimmune diseases. Each autoimmune disease is a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): B01J20/26B01J20/30B01D15/22
Inventor 张旭锋李树兴
Owner 李树兴
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap