Enclosed photobioreactor apparatus based on process mass spectrometer, and alga cell growth process monitoring method

A technology of photobioreactor and process mass spectrometer, which is applied in the direction of photobioreactor, specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, etc. Plant physiological metabolic intensity, lack of methods for rapid and accurate determination of phytoplankton primary productivity or physiological metabolic intensity, etc.

Active Publication Date: 2012-08-01
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods also cannot quickly and accurately measure the physiological metabolic intensity of phytoplankton (algae) online.
[0011] In summary, there is currently a lack of rapid and accurate methods for determining the primary productivity or physiological metabolic intensity of phytoplankton (algae) in this area, so it is urgent to develop an online method that can accurately measure phytoplankton (algae) in milliseconds. Methods and apparatus for primary productivity or physiological metabolic strength

Method used

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  • Enclosed photobioreactor apparatus based on process mass spectrometer, and alga cell growth process monitoring method
  • Enclosed photobioreactor apparatus based on process mass spectrometer, and alga cell growth process monitoring method
  • Enclosed photobioreactor apparatus based on process mass spectrometer, and alga cell growth process monitoring method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Observation and Analysis of Indoor Cultivation Process of Cyanobacteria in Taihu Lake

[0081] device:

[0082] like figure 1 The enclosed photobioreactor device of the present invention is constructed as shown. Among them, a 50L fermenter (Shanghai Guoqiang Biochemical) was used, such as figure 2 Assemble as shown; image 3 The exhaust gas analysis pretreatment system is constructed as shown, connected to the downstream of the photoreactor and the upstream of the mass spectrometer (U.S. Extrel MAX300-LG); as Figure 4 The data acquisition and processing system is constructed and connected as shown, the process control system adopts Bioradar (Shanghai Guoqiang Biochemical), and the data acquisition system adopts Biostar (Shanghai Guoqiang Biochemical). Perform parameter setting and operation according to the manufacturer / supplier's instructions of the equipment and software.

[0083] Training method:

[0084] Take water and sediment from Meiliang Bay, Taihu Lake; s...

Embodiment 2

[0092] Observation results of cyanobacteria cultivation at the same Taihu Lake location in different seasons of water intake

[0093] With reference to the description in Example 1, water and mud were taken from Taihu Lake in late March, mid-April, and late April, 2008, respectively, for cultivation and observation of algae cell growth.

[0094] The result is as Image 6 shown.

[0095] Depend on Image 6 A It can be seen that the growth rate of cyanobacteria is significantly affected by the time of water intake. The Taihu Lake water taken in late April began to grow rapidly at about 20 hours, and chlorophyll a reached the highest value at about 90 hours. However, the Taihu Lake water collected in mid-April did not begin to grow rapidly until 50 hours, and the recovery period of Taihu Lake water in late March was the longest, about 70 hours.

[0096] The peak time of chlorophyll a and CFR was also delayed compared with the experiment in late April ( Image 6 B).

Embodiment 3

[0098] Chlorella culture online monitoring results

[0099] Referring to the description in Example 1, in this example, Chlorella was used as the culture object, and the photosynthetic culture was carried out with the inorganic medium, and the culture temperature was 30 degrees.

[0100] The formula of inorganic salt medium is as follows (g / L):

[0101] KNO 3

1.25，

K H 2 PO 4

1.25g，

MgSO 4 ·7H 2 o

1.0g，

CuSO 4 2H 2 o

0.0835g

FeSO 4 ·7H 2 o

0.049g

EDTA

0.05mg

trace elements

10ml

[0102] On-line analysis of CO by mass spectrometry during Chlorella culture 2 Exhaust concentration, then according to this device for CO 2 Constant rate (CFR) for calculation, while sampling optical density (OD 680 ) to analyze the growth density of Chlorella.

[0103] The results of the analysis of the two Figure 7 shown. The results of linear correlation analysis showed that the...

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Abstract

The invention relates to an enclosed photobioreactor apparatus based on a process mass spectrometer, and an alga cell growth process monitoring method. The enclosed photobioreactor apparatus provided in the invention comprises: an enclosed photobioreactor which comprises a culture tank, a light source providing illumination, a stirring system, a gas inlet pipeline, and a gas outlet pipeline; and an online process mass spectrometer system which is connected or integrated with the enclosed photobioreactor, and is used for analyzing the outlet gas. The apparatus and the method have the characteristics of directness, real time, accuracy and the like.

Description

technical field [0001] The present invention relates to the fields of environmental monitoring and environmental protection. Specifically, the present invention relates to a closed photobioreactor device based on a process mass spectrometer and a method for monitoring the growth process of algae cells. The method and equipment of the present invention can be used to monitor lakes and ocean cyanobacteria , microalgae and other algal cell growth. Background technique [0002] Algal cells such as cyanobacteria and microalgae use carbon dioxide and light energy to synthesize sugars necessary for cell growth through photosynthesis and release oxygen to synthesize macromolecules such as proteins, fats, and nucleic acids. Whether it is cyanobacteria, diatoms and microalgae grown in open lakes, oceans and rivers, ponds, sewage ponds, etc., or artificially cultivated in closed photobioreactors (pipeline, flat, vertical hanging bags, floating The most basic physiological feature of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/34C12M1/02C12N1/12
CPCC12M21/02C12M41/00C12M41/40C12M41/34C12M41/12C12M41/06C12M27/02C12M29/20C12M41/28
Inventor 郭美锦赵策
Owner EAST CHINA UNIV OF SCI & TECH
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