Preparation method of decolorized squid skin collagen

A technology for skin collagen and squid skin, which is applied in the field of protein materials in biotechnology, can solve the problems of difficult removal of activated carbon, limited application of collagen peptides, and influence on collagen processing, etc., and achieves improved processing and utilization level, good decolorization effect, and collagen loss rate. low effect

Inactive Publication Date: 2012-08-08
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current extraction process of squid skin collagen includes alkaline extraction, acid extraction and enzyme extraction, but because squid skin collagen contains a certain amount of pigment, the extracted collagen is dark red, which greatly limits the application of collagen peptides , therefore, squid...

Method used

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  • Preparation method of decolorized squid skin collagen
  • Preparation method of decolorized squid skin collagen
  • Preparation method of decolorized squid skin collagen

Examples

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preparation example Construction

[0011] The present invention is the preparation method of decolorized squid skin collagen, and its steps are:

[0012] (1) Take relatively complete frozen squid skin, wash it with deionized water, and dry it naturally;

[0013] (2) Get dried squid skin, be placed in the container, add the potassium permanganate solution that concentration is 1%, be 1: 20~30 ratio g / ml by the quality of squid skin and the volume ratio g / ml of potassium permanganate solution Determine the volume of the potassium permanganate solution added, stir evenly, and the stirring time lasts 60 minutes;

[0014] (3) remove the potassium permanganate solution in the container, clean the squid skin 3 times, then put the squid skin in the container, add the oxalic acid solution that the concentration is 8%, press the quality of the squid skin and the volume ratio of the oxalic acid solution The ratio of g / ml is 1:20-30 to determine the volume of the added oxalic acid solution, stir evenly, and the stirring t...

Embodiment 1

[0018] Take 50 g of dried squid skin, place it in a container, add 1 L of 1% potassium permanganate solution, and stir evenly for 60 minutes with an electric stirrer at a speed of 50 to 60 rpm. Remove the potassium permanganate solution in the container, wash the squid skin with 250ml of distilled water for 3 times, then place it in the container, add 1L of oxalic acid solution with a concentration of 8%, and stir evenly with an electric stirrer at a speed of 50-60 rpm 6 hours. Remove the oxalic acid solution in the container, and squid skin is cleaned 3 times with 250ml distilled water. Homogenize the squid skin, place it in a container, add 1L of distilled water, and then add 0.25g trypsin, then place the container where the squid skin is placed in a water bath with a constant temperature of 45°C, and hydrolyze it for 2 hours to obtain a decolorized collagen solution. The decolorized collagen solution was freeze-dried in a vacuum freeze dryer.

[0019] (1) if figure 1 , ...

Embodiment 2

[0031] Take 500 g of dried squid skin, place it in a container, add 10 L of 1% potassium permanganate solution, and stir evenly for 60 minutes with an electric stirrer at a speed of 50 to 60 rpm. Remove the potassium permanganate solution in the container, wash the squid skin with 2500ml of distilled water for 3 times, then place it in the container, add 10L of oxalic acid solution with a concentration of 8%, and stir evenly with an electric stirrer at a speed of 50-60 rpm 6 hours. Remove the oxalic acid solution in the container, and squid skin is cleaned 3 times with 2500ml distilled water. Homogenize the squid skin, place it in a container, add 1L of distilled water, and then add 2.5g of trypsin, place the container containing the squid skin in a water bath at a constant temperature of 45°C, and hydrolyze it for 2 hours to obtain the final decolorizing solution. The collagen solution was freeze-dried in a vacuum freeze dryer.

[0032] (1) if Figure 5 , Figure 6 As sho...

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Abstract

Disclosed is a preparation method of decolorized squid skin collagen, comprising the following steps of: taking dry squid skin, adding a potassium permanganate solution which accounts for 20-30 times the weight of the squid skin and has a concentration of 1%, uniformly stirring for 60 minutes at the speed of 50-60r/m by the use of an electric mixer, pouring potassium permanganate raffinate, cleaning for 3 times by the use of water which accounts for 3-5 times the weight of the squid skin, adding an oxalic acid solution which accounts for 20-30 times the weight of the squid skin and has a concentration of 8%, uniformly stirring for 6-6.5 hours at the speed of 50-60r/m by the use of the electric mixer, pouring oxalic acid raffinate, cleaning for 3 times by the use of distilled water which accounts for 3-5 times the weight of the squid skin, homogenizing the squid skin, adding distilled water which accounts for 20 times the weight of the squid skin, adding trypsin which accounts for 0.5-0.6% of the weight of the squid skin, placing into a constant-temperature water-bath kettle of 45 DEG C for water bath for 2 hours to obtain the final decolorized collagen liquid, and placing the decolorized collagen liquid into a lyophilizer for freeze-drying.

Description

technical field [0001] The invention belongs to the field of protein materials in biotechnology, and in particular relates to a preparation method of squid skin collagen. Background technique [0002] my country has vast waters, well-developed fishery, and squid resources are advancing by leaps and bounds. However, squid accounts for 8-13% of squid skin waste during processing. Most factories adopt the method of burying and discarding these wastes, which not only causes a great waste of resources, but also directly leads to environmental pollution. Collagen is extracted from squid processing waste squid skin to prepare collagen polypeptide, which can not only reduce environmental pollution, but also improve the economic benefits of enterprises and improve the processing and utilization level of aquatic products. The current extraction process of squid skin collagen includes alkaline extraction, acid extraction and enzyme extraction, but because squid skin collagen contains ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 张伟杰张飞付万冬王永刚杨明俊
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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