Preparation method of decolorized squid skin collagen

A technology for skin collagen and squid skin, which is applied in the field of protein materials in biotechnology, can solve the problems of difficult removal of activated carbon, limited application of collagen peptides, and influence on collagen processing, etc., and achieves improved processing and utilization level, good decolorization effect, and collagen loss rate. low effect
CN102628071AInactive Publication Date: 2012-08-08LANZHOU UNIVERSITY OF TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
LANZHOU UNIVERSITY OF TECHNOLOGY
Publication Date
2012-08-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

Disclosed is a preparation method of decolorized squid skin collagen, comprising the following steps of: taking dry squid skin, adding a potassium permanganate solution which accounts for 20-30 times the weight of the squid skin and has a concentration of 1%, uniformly stirring for 60 minutes at the speed of 50-60r / m by the use of an electric mixer, pouring potassium permanganate raffinate, cleaning for 3 times by the use of water which accounts for 3-5 times the weight of the squid skin, adding an oxalic acid solution which accounts for 20-30 times the weight of the squid skin and has a concentration of 8%, uniformly stirring for 6-6.5 hours at the speed of 50-60r / m by the use of the electric mixer, pouring oxalic acid raffinate, cleaning for 3 times by the use of distilled water which accounts for 3-5 times the weight of the squid skin, homogenizing the squid skin, adding distilled water which accounts for 20 times the weight of the squid skin, adding trypsin which accounts for 0.5-0.6% of the weight of the squid skin, placing into a constant-temperature water-bath kettle of 45 DEG C for water bath for 2 hours to obtain the final decolorized collagen liquid, and placing the decolorized collagen liquid into a lyophilizer for freeze-drying.
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Description

technical field

[0001] The invention belongs to the field of protein materials in biotechnology, and in particular relates to a preparation method of squid skin collagen. Background technique

[0002] my country has vast waters, well-developed fishery, and squid resources are advancing by leaps and bounds. However, squid accounts for 8-13% of squid skin waste during processing. Most factories adopt the method of burying and discarding these wastes, which not only causes a great waste of resources, but also directly leads to environmental pollution. Collagen is extracted from squid processing waste squid skin to prepare collagen polypeptide, which can not only reduce environmental pollution, but also improve the economic benefits of enterprises and improve the processing and utilization level of aquatic products. The current extraction process of squid skin collagen includes alkaline extraction, acid extraction and enzyme extraction, but because squid skin collagen contains ...

Claims

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