Method for foot-and-mouth disease vaccine concentration and purification
A technology of foot-and-mouth disease vaccine and purification method, which is applied in chemical instruments and methods, peptide preparation methods, organic chemistry, etc., can solve the problems of vaccine safety and unstable immune effect, and achieve less adverse reactions and better immune effect
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[0019] Example 1: Manufacture of foot-and-mouth disease O-type inactivated vaccine, using foot-and-mouth disease OZK / 93 strain and OR / 80 strain to inoculate BHK21 cell suspension culture, harvest the cell culture, and then inactivate it with diethyleneimine (BEI) After treatment with the following methods, add mineral oil adjuvant and emulsify to make a vaccine for the prevention of swine O-type foot-and-mouth disease.
[0020] (1) Use a continuous flow centrifuge to remove large particulate matter in the antigen
[0021] ①Using a continuous flow centrifuge, set the speed to 3000 rpm.
[0022] ② Collect the light liquid discharged from the centrifuge and discard the sediment (heavy liquid).
[0023] (2) Treat with a hollow fiber ultrafiltration system (1) Antigen solution after step
[0024] ① The hollow fiber used for the concentration of foot-and-mouth disease virus has a molecular weight of 50K, a pore size of 0.5mm, and a quantity of 3000 fibers per membrane column. The number of h...
Example Embodiment
[0030] Example 2: Manufacture of foot-and-mouth disease O-type inactivated vaccine, using foot-and-mouth disease OZK / 93 strain and OR / 80 strain to inoculate BHK21 cell suspension culture, harvest the cell culture, and inactivate it with diethyleneimine (BEI) After treatment with the following methods, add mineral oil adjuvant and emulsify to make a vaccine for the prevention of swine O-type foot-and-mouth disease.
[0031] (1) Use a continuous flow centrifuge to remove large particulate matter in the antigen
[0032] ①Using a continuous flow centrifuge, set the speed to 8000 rpm.
[0033] ② Collect the light liquid discharged from the centrifuge and discard the sediment (heavy liquid).
[0034] (2) Treat with a hollow fiber ultrafiltration system (1) Antigen solution after step
[0035] ① The hollow fiber used for the concentration of foot-and-mouth disease virus has a molecular weight specification of 100K, a pore size of 1.5mm, and a quantity of 2000 fibers per membrane column. The n...
Example Embodiment
[0041] Example 3: Manufacture of foot-and-mouth disease O-type inactivated vaccine, using foot-and-mouth disease OZK / 93 and OR / 80 strains to inoculate BHK21 cell suspension culture, harvest the cell culture, and then inactivate it with diethyleneimine (BEI) After treatment with the following methods, add mineral oil adjuvant and emulsify to make a vaccine for the prevention of swine O-type foot-and-mouth disease.
[0042] (1) Use a continuous flow centrifuge to remove large particulate matter in the antigen
[0043] ①Using a continuous flow centrifuge, set the speed to 5000 rpm.
[0044] ② Collect the light liquid discharged from the centrifuge and discard the sediment (heavy liquid).
[0045] (2) Treat with a hollow fiber ultrafiltration system (1) Antigen solution after step
[0046] ①The hollow fiber used in the concentration of foot-and-mouth disease virus has a molecular weight specification of 300K, a pore size of 2.0mm, and a quantity of 1500 fibers per membrane column. The numb...
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