Method for identifying Alternaria altemata (Fr.) Keissler resistance of pear germplasm

A pear black spot and anti-black spot technology, applied in horticultural methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of pathogenic bacteria resistance to chemical agents, environmental pollution, human health, and harm, etc. Achieve the effects of simple operation, easy method and accurate identification results

Inactive Publication Date: 2012-08-22
INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
View PDF4 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current method of preventing and treating pear black spot is to use chemical agents such as thiophanate-methyl, mancozeb and difenoconazole. If these chemical agents are used for a lo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying Alternaria altemata (Fr.) Keissler resistance of pear germplasm
  • Method for identifying Alternaria altemata (Fr.) Keissler resistance of pear germplasm
  • Method for identifying Alternaria altemata (Fr.) Keissler resistance of pear germplasm

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0060] A method for identifying pear germplasm against black spot disease, the steps are:

[0061] 1. Preparation of black spot pathogens:

[0062] (1) Preparation of culture medium:

[0063] Potato culture medium (PSA): 200g potato (peeled), 20g agar powder, 20g sucrose, 1000mL water. The preparation method is as follows: peel the potato and cut into about 2cm 2 Boil for 0.5h, stirring with a glass rod to prevent paste. Then filter with double gauze, take the filter solution and add agar powder, add sucrose after dissolution, and then make up water to 1000mL, pH 7.0.

[0064] (2) Isolation and identification of pear black spot disease:

[0065] The collected leaves or fruits with pear black spot disease were carried out with reference to the "Microbiology Experimental Techniques" edited by Du Lianxiang and Lu Fuping in 2005 for tissue separation, monospore purification, culture and preservation. Inoculate the isolated strains on young pear leaves to observe whether typical black spo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for identifying Alternaria altemata (Fr.) Keissler resistance of pear germplasm, which comprises the following steps: (1) preparing Alternaria altemata (Fr.) Keissler pathogen, and mainly performing culture medium preparation, Alternaria altemata (Fr.) Keissler separation identification, Alternaria altemata (Fr.) Keissler pathogen transfer and Alternaria altemata (Fr.) Keissler pathogen activation and enlarging culture; (2) preparing a Alternaria altemata (Fr.) Keissler resistance material, and mainly performing pear variety grafting and pear grafting variety transplanting; (3) performing artificial inoculation identification, and mainly utilizing Alternaria altemata (Fr.) Keissler pathogen spore suspension to perform spraying inoculation to grafted seedling blades of an annual pear variety; and (4) performing operation of disease investigation and grading standard. The method is practical and simple and convenient to operate, and the Alternaria altemata (Fr.) Keissler resistance of the pear variety can be identified rapidly and accurately. By means of the method, the Alternaria altemata (Fr.) Keissler resistance of the pear variety in scientific research, breeding and production of pears is identified rapidly.

Description

technical field [0001] The invention belongs to the technical field of fruit trees, and more specifically relates to a method for identification of pear germplasm resistance to black spot disease. The method can effectively solve the problem of fast identification of pear variety resistance to black spot disease in pear scientific research, breeding and production. This method is suitable for scientific research units, companies and individuals engaged in pear scientific research, breeding and production. Background technique [0002] Pear belongs to Rosaceae (Rosaceae) Apple subfamily (Maloideae) Pyrus (Pyrus) plant, is the deciduous fruit tree of Qiaoben, is one of three major cultivated fruit trees in my country, and also occupies a pivotal position in the world. According to the statistics of China Yearbook, in 2010, the pear planting area in my country was 10.631 million hectares, and the output was 15.0571 million tons, ranking first in the world. Pear production has ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G7/06A01G17/00C12Q1/18
Inventor 胡红菊杨晓平陈启亮田瑞张靖国
Owner INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap