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Method for supercritically extracting natural activity ingredient oil tea polyphenol from oil tea fruits

An active ingredient, supercritical technology, applied in the direction of medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., to achieve the effect of simple process, less usage, and improved extraction rate

Inactive Publication Date: 2012-08-22
TIANJIN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patents CN101194713 and CN102041164A respectively relate to methods for extracting camellia oil using camellia oleifera seeds as raw materials, using supercritical extraction technology or ultrasonic-assisted subcritical extraction technology, but none of them have further developed and utilized camellia oleifera

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0014] Shell and separate camellia oleifera fruit, weigh 300g of camellia oleifera seeds, put them in an oven and dry for 1h, and obtain 293.8g of camellia oleifera seeds after cooling, crush the dried camellia oleifera seeds to 50 mesh, add to the extraction kettle, and extract under supercritical CO? It is: temperature 45 ℃, pressure 30 MPa, CO flow rate 120 L / h, extraction 2h, the light yellow transparent camellia oil obtained by supercritical extraction is collected in the separation tank, its mass is 88.9 g, and the extraction rate of camellia oil is 29.63%.

[0015] Adjust the supercritical extraction conditions, the pressure is 20 MPa, and the temperature is constant, add 70% ethanol aqueous solution to the extraction kettle at a rate of 0.03 L / h, extract for 1.5 h, and collect the extract in the separation tank. The method of rotary evaporation was used to remove the entraining agent in the extract at 55 °C to obtain a light yellow powdery solid of camellia oleifera po...

example 2

[0017] The camellia oleifera fruit was shelled and separated, 500 g of camellia oleifera seeds were weighed, dried in an oven for 3 h, and 487.5 g of camellia oleifera seeds were obtained after cooling, and the dried camellia oleifera seeds were crushed to 40 mesh, added to the extraction kettle, supercritical CO ?Extraction conditions are: temperature 55 ℃, pressure 30 MPa, CO? flow rate 120 L / h, extraction 3h, the light yellow tea oil obtained by supercritical extraction is collected in the separation tank, its mass is 141.8 g, tea oil extraction The rate is 28.35%.

[0018] Adjust the supercritical extraction conditions, the pressure is 25 MPa, the temperature is constant, the ethyl acetate entrainer is injected into the extraction kettle at a rate of 0.07 L / h, the extraction time is 2 h, and the extract is collected. The method of rotary evaporation was used to remove the entraining agent in the extract at 60 °C to obtain a light yellow powdery solid of camellia oleifera p...

example 3

[0020] The camellia oleifera fruit was shelled and separated, 800 g of camellia oleifera seeds were weighed, dried in an oven for 3 h, and 777.5 g of camellia oleifera seeds were obtained after cooling, the dried camellia oleifera seeds were crushed to 60 mesh, put into an extraction kettle, and supercritical CO? The extraction conditions are: temperature 45°C, pressure 35 MPa, CO? flow rate 150 L / h, extraction time 4h, the light yellow transparent camellia oil obtained by supercritical extraction is collected in the separation tank, its mass is 249.4g, the camellia oil extraction The rate is 31.17%.

[0021] Adjust the supercritical extraction conditions, the pressure is 25 MPa, the temperature is constant, 60% ethanol aqueous solution is added to the extraction kettle at a rate of 0.16 L / h, the extraction time is 1 h, and the extract is collected. The method of rotary evaporation was used to remove the entraining agent in the extract at 55 °C to obtain a light yellow powdery...

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Abstract

The invention discloses a method for supercritically extracting natural activity ingredient oil tea polyphenol from oil tea fruits, which belongs to the technical field of oil tea polyphenol extraction. The method comprises the following steps: oil tea fruits are used as raw materials to be dried and smashed; supercritical CO2 is used for removing tea oil from oil tea seeds, oil-removed oil tea seeds are mixed with hulls or used independently, oil tea fruit hulls are used as raw materials to supercritically extract oil tea polyphenol, and an entrainer is injected in dynamic entrainment mode to perform supercritical CO2 extraction to achieve oil tea polyphenol. Compared with a traditional solvent formulation method, the method is high in utilization rate of resources, low in toxicity of the entrainer in the whole process, small in using amount, simple in process and environment-friendly.

Description

technical field [0001] The invention relates to a method for supercritically extracting camellia oleifera polyphenols, a natural active ingredient, from camellia oleifera fruit, and belongs to the technical field of camellia oleifera polyphenols extraction technology. Background technique [0002] Camellia oleifera is a kind of woody edible oil tree species unique to China, and is known as the world's four largest woody oil plants together with olive oil, coconut, and oil palm. At present, the extraction of active ingredients in camellia oleifera mainly focuses on camellia oleifera saponin, but with the deepening of research, many functions of camellia oleifera polyphenols have been discovered. Tea polyphenols have good antioxidant properties due to their high reactivity of hydroxyl substitution and their ability to phagocytose free radicals. Studies have found that Camellia oleifera not only has good antioxidant properties, but also delays the aging of the body and inhibi...

Claims

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Application Information

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IPC IPC(8): A61K36/82A61P39/06A61P35/00A61P27/12A61P9/10A61P13/12A23L3/3472
Inventor 唐韶坤池建苏伊文峰白杨
Owner TIANJIN UNIV