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Ionic liquid functionalized magnetic nanoparticle and preparation method and application thereof

A technology of magnetic nanoparticles and ionic liquids, which is applied in peptide preparation methods, chemical instruments and methods, microsphere preparation, etc., to achieve good separation effect, simple and convenient operation, and short separation time

Inactive Publication Date: 2012-08-22
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the application of ionic liquid functionalized magnetic particles to protein separation has not been reported. Through the selective electrical action and complexation of the functional particles on proteins, it can be realized according to the acidity and alkalinity of the protein and whether it has a heme group. Highly selective and high-capacity separation and purification of hemoglobin and other proteins

Method used

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  • Ionic liquid functionalized magnetic nanoparticle and preparation method and application thereof
  • Ionic liquid functionalized magnetic nanoparticle and preparation method and application thereof
  • Ionic liquid functionalized magnetic nanoparticle and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] (1) Fe 3 o 4 Preparation of Magnetic Nanoparticles

[0033] FeCl 3 .6H 2O1g, 2g of anhydrous sodium acetate dissolved in 40mL of ethylene glycol, put into a 50mL reactor and react at 200°C for 6h, after cooling, wash with ethanol and deionized water ultrasonically three times respectively, use an external magnetic field to separate, and finally use ethanol ultrasonically Preserve after dispersion to get Fe 3 o 4 magnetic nanoparticles.

[0034] (2) Fe 3 o 4 / SiO 2 Preparation of core-shell magnetic nanoparticles: weighing (1) product Fe 3 o 4 0.1g, add H 2 O and ethanol (EtOH), mixed at a volume ratio of 1 / 4, ultrasonically dispersed for 10 minutes, passed through N 2 , add NH under stirring 3 .H 2 O to adjust the pH to 9, add 0.1mL tetraethyl orthosilicate (TEOS) to the solution after heating up, and react at constant temperature for 1h, transfer the reaction mixture to a centrifuge tube for centrifugation, wash with deionized water and EtOH, and vacuum d...

Embodiment 2

[0041] (1) Fe 3 o 4 Preparation of Magnetic Nanoparticles: FeCl 3 .6H 2 O1g, 2g of anhydrous sodium acetate was dissolved in 40mL of ethylene glycol, put into a 50mL reactor and reacted at 200°C for 6h, after cooling, ultrasonically washed three times with ethanol and deionized water respectively, separated by an external magnetic field, and finally dispersed with ethanol ultrasonically After saving to get Fe 3 o 4 magnetic nanoparticles.

[0042] (2) Fe 3 o 4 / SiO 2 Preparation of core-shell magnetic nanoparticles: weighing (1) product Fe 3 o 4 0.1g, add H 2 O and ethanol (EtOH), mixed at a volume ratio of 1 / 4, ultrasonically dispersed for 10 minutes, passed through N 2 , add NH under stirring 3 .H 2 O to adjust the pH to 9, add 0.1mL tetraethyl orthosilicate (TEOS) to the solution after heating up, and react at constant temperature for 1h, transfer the reaction mixture to a centrifuge tube for centrifugation, wash with deionized water and EtOH, and vacuum dry at...

Embodiment 3

[0047] (1) Fe 3 o 4 Preparation of Magnetic Nanoparticles: FeCl 3 .6H 2 O1g, 2g of anhydrous sodium acetate was dissolved in 40mL of ethylene glycol, put into a 50mL reactor and reacted at 200°C for 6h, after cooling, ultrasonically washed three times with ethanol and deionized water respectively, separated by an external magnetic field, and finally dispersed with ethanol ultrasonically After saving to get Fe 3 o 4 magnetic nanoparticles.

[0048] (2) Fe 3 o 4 / SiO 2 Preparation of core-shell magnetic nanoparticles: weighing (1) product Fe 3 o 4 0.1g, add H 2 O and ethanol (EtOH), mixed at a volume ratio of 1 / 4, ultrasonically dispersed for 10 minutes, passed through N 2 , add NH under stirring 3 .H 2 O to adjust the pH to 9, add 0.1mL tetraethyl orthosilicate (TEOS) to the solution after heating up, and react at constant temperature for 1h, transfer the reaction mixture to a centrifuge tube for centrifugation, wash with deionized water and EtOH, and vacuum dry at...

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Abstract

The invention relates to an ionic liquid functionalized magnetic nanoparticle and a preparation method and application thereof. FeCl3.6H2O and anhydrous sodium acetate are dissolved in glycol, and react under 200 DEG C, so that Fe3O4 magnetic nanoparticles are obtained; the Fe3O4 magnetic nanoparticles are weighed and mixed with H2O and ethanol, pH is regulated to 9 to 10, tetraethoxysilane is added for reaction, and after vacuum drying, Fe3O4 / SiO2 is obtained; the Fe3O4 / SiO2 is dispersed and suspended in toluene, and after 3-chloropropyltrimethylsilane and triethylamine are added for reaction, chloropropyl magnetic nanoparticles are obtained by way of vacuum drying; the chloropropyl magnetic nanoparticles are added into toluene, N-alkylimidazole derivative is added for reaction, and after vacuum drying, the ionic liquid functionalized magnetic nanoparticles are obtained. When being used for separating target standard protein, the ionic liquid functionalized magnetic nanoparticle has the characteristics of good selectivity and high adsorbing capacity, and cannot contaminate target separated product.

Description

technical field [0001] The invention relates to an ionic liquid functionalized magnetic nano particle and a preparation method and application thereof. Background technique [0002] With the completion of the Human Genome Project, life science research has ushered in the post-genome era, and the door of proteomics has opened. Due to the physical and chemical properties of proteins, there is not only no general research technology applicable to most proteins, but also no amplification technology like DNA, so it is very difficult to obtain sufficient quantities of high-purity proteins. In order to increase the purity or specific activity of protein products and improve the efficiency, speed, yield and purity of protein separation, it is necessary to separate the target protein from all other components of the cell, especially miscellaneous proteins, while still retaining the biological activity and chemical integrity. At present, protein separation technology mainly focuses ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02C07K1/14
Inventor 魏芸李言
Owner BEIJING UNIV OF CHEM TECH
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