Preparation method of water-soluble cholesterol lyophilized product derived from serum
A cholesterol and water-soluble technology, which is applied in the field of preparation of water-soluble cholesterol freeze-dried products, can solve problems such as difficulty in obtaining purity, affecting product quality, and poor stability, and achieves the effects of simple operation, high recovery rate, and good repeatability
Active Publication Date: 2014-09-10
AILEX TECH GRP CO LTD +1
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Problems solved by technology
Since serum contains a certain concentration of water-soluble cholesterol, these water-soluble cholesterol is either combined with certain compounds or combined with lipoproteins, so it has strong water solubility. In view of this, some companies currently use high-concentration serum as a preparation There are also some high-concentration cholesterol concentrates obtained by concentrating serum, but the defects of these methods are: first, it is difficult to obtain a product with higher purity, and its serum matrix content is very high, thereby affecting the quality of the product; again, due to It is a liquid product with poor stability
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[0013] The invention provides a serum-derived freeze-dried product rich in water-soluble cholesterol. The freeze-dried product provided by the invention has good stability, and the liquid is clear after reconstitution without foreign matter or insoluble matter, and the water-soluble cholesterol will neither significantly interfere with the detection of routine items nor affect the stability of other components Sex matters.
[0014] The present invention will be further described below by way of examples.
[0015] The present invention is a preparation method of water-soluble cholesterol freeze-dried product derived from serum, which adopts the following steps: (A) Taking 1L serum as an example, adjust its pH value to 6.9 with 1mol / L sodium hydroxide, and take 150ml Add absolute ethanol dropwise to the continuously stirring serum, stir at room temperature for 2 hours, centrifuge at 1000g for 10 minutes, and collect the precipitate;
[0016] (B) Weigh 0.3g dextran and 6.5g cal...
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The invention relates to a cholesterol lyophilized product, and in particular relates to a preparation method of a water-soluble cholesterol lyophilized product derived from serum. The preparation method comprises the following steps of: adjusting pH value of the serum to 6.9 by utilizing 1mol / L of sodium hydroxide, taking 150ml of absolute ethyl alcohol for dropwise adding to the continuously stirred serum, stirring the serum for 2h at the room temperature, centrifuging the serum for 10min per 1000g and collecting precipitates; weighing 0.3g of glucan and 6.5g of calcium chloride, dissolving the glucan and the calcium chloride to 500ml of deionized water, dissolving the precipitates collected from the former step by the solution, stirring the solution for 2h at the room temperature, centrifuging the solution for 10min per 1000g and collecting the precipitates again; and redissolving the collected precipitates and lyophilizing the precipitates to obtain a cholesterol- rich lyophilized product. Compared with the prior art, with the adoption of the preparation method, cholesterol is lyophilized and then is redissolved by the deionized water, the appearance is clear and bright, the purity of the cholesterol is high, the repeatability is good, and the recovery rate is high.
Description
[technical field] [0001] The invention relates to a freeze-dried product of cholesterol, in particular to a method for preparing a freeze-dried product of water-soluble cholesterol derived from blood serum used in biological and medical laboratory research. [Background technique] [0002] Cholesterol, also known as cholesterol, has the chemical formula C 27 h 46 O, is a steroid and sterol. Cholesterol is an organic compound with poor water solubility. The solubility of pure cholesterol in water is only about 0.095mg / L (30°C). would be difficult to add. Since serum contains a certain concentration of water-soluble cholesterol, these water-soluble cholesterol is either combined with certain compounds or combined with lipoproteins, so it has strong water solubility. In view of this, some companies currently use high-concentration serum as a preparation There are also some high-concentration cholesterol concentrates obtained by concentrating serum, but the defects of these m...
Claims
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IPC IPC(8): G01N1/28
Inventor 李子樵王青
Owner AILEX TECH GRP CO LTD