Preparation method for high-sensitivity amperometric glucose sensor

A glucose sensor and high-sensitivity technology, applied in the direction of material electrochemical variables, etc., can solve the problems that the reaction is affected by temperature and pH value, the stability is limited, and the storage capacity has no significant change.

Inactive Publication Date: 2012-08-22
WUXI BAILING SENSING TECH
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymes are highly selective, but at the same time have a number of disadvantages: their stability is limited and their reactions are dependent on temperature and pH
[0005] In fact, conventional electrochemical

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with implementation.

[0015] A method for preparing a high-sensitivity current-type glucose sensor, the steps of which are as follows:

[0016] (1) Preparation of α-Ni(OH) 2 The sol and nanostructure film: firstly, at 50°C, dissolve 9.64mmol of nickel acetate tetrahydrate in 25ml of glycerol and stir to obtain solution A, and dissolve 19.28mmol of KOH in 19.3ml of n-butanol to obtain a solution B; then solution B is added to solution A to form a mixed solution; the mixed solution formed is stirred at room temperature for 6h to form a bright green transparent suspension, which is used to prepare nano-scale stable α-Ni ( Oh) 2 Modified fluorine-doped tin oxide (FTO) electrodes;

[0017] (2) The cut FTO electrodes (R=15Ω) were ultrasonically washed with isopropanol and methanol for 15 minutes and then dried in the air, with each electrode ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method for a high-sensitivity amperometric glucose sensor. Alpha-Ni(OH)2 sol and a nanostructured membrane are first prepared, cut FTO (fluorine-doped tin oxide) electrodes are then ultrasonically washed sequentially with isopropanol and methanol for 15 minutes, and are dried in the air, and under the rotational speed of 2500rpm, the spin-coating method is adopted to spin-coat the prepared Alpha-Ni(OH)2 sol on each electrode within 1.0cm2 of limited area for 2 minutes; finally, the electrode is dried under vacuum for 24 hours and then roasted under 240 DEG C for 30 minutes, and thereby the nanostructured membrane-modified electrochemical sensor formed by Alpha-Ni(OH)2 is obtained. Cyclic voltammetry and flow injection analysis indicate that the sensor shows enlarged surface area and enhanced electrocatalytical property.

Description

Technical field: [0001] The invention relates to a preparation method of a glucose sensor, in particular to a preparation method of a high-sensitivity current type glucose sensor. Background technique: [0002] Glucose is a very important metabolite, and its monitoring is a fundamental problem in the fields of biomedicine, food, beverage and biochemical technology. Near-infrared spectroscopy is commonly used in industry to estimate the concentration of glucose, but this method is very expensive and impractical for routine monitoring, thus promoting the development of simple and inexpensive electrochemical sensors. [0003] Amperometric glucose sensors can be classified into two types: enzymatic and non-enzymatic. For the enzymatic glucose sensor, glucose oxidase is immobilized on the surface of the electrode, and molecular oxygen is reduced to hydrogen peroxide while glucose is oxidized, which is then monitored in the form of electric current. Enzymes are highly selective,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/26
Inventor 孙京华潘正海
Owner WUXI BAILING SENSING TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap