Three fungi capable of inducing aquilaria sinensis to produce agilawood
A technology of white wood, fungus, applied in the field of bioengineering
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Embodiment 1
[0021] Application of Fungus YNAS-4 to Induce Agarwood to Produce Agarwood
[0022] (1) Preparation of solid medium: prepare a suspension with 0.5% concentration of sucrose and calcium sulfate; mix the three materials of buckwheat husk, wheat bran and corn flour in a volume ratio of 1:1:0.05 ; Add 1 liter of suspension per kilogram of dry material and stir evenly. After the medium was dispensed, it was sterilized at 121°C for 165 minutes.
[0023] (2) Preparation of strains: Pick the strain block at the edge of the YNAS-4 colony activated on the PDA plate, inoculate it into a canned bottle equipped with the above-mentioned solid substrate, and culture in dark at 22-24° C. for 15 days. The vigorously growing fungal hyphae block was transferred to the canned bottle with buckwheat husk culture medium again, cultivated in dark at 22-24° C. for 30 days, and set aside.
[0024] (3) Preparation of the inoculation hole: select the white wood incense tree with a tree age of 5 years a...
Embodiment 2
[0028] Application of Fungus YNAS-6 to Induce Agarwood to Produce Agarwood
[0029] (1) The making of solid culture medium: preparation glucose concentration is 1.2%, and calcium sulfate concentration is the suspension of 1%; These 3 kinds of materials of buckwheat husk, wheat bran and corn flour are 2: 1.5: 0.07 by volume ratio The ratio is mixed evenly; add 0.8 liters of suspension per kilogram of dry material, and stir evenly. After aliquoting the medium, it was sterilized at 121°C for 140 minutes.
[0030] (2) Preparation of strains: Pick the strain block at the edge of the YNAS-6 colony activated on the PDA plate, inoculate it into the canned bottle with the above-mentioned solid substrate, and culture in dark at 25-28° C. for 15 days. The vigorously growing fungal hyphae block was transferred to the can bottle equipped with buckwheat husk medium again, and cultured in dark at 25-28° C. for 20 days, and set aside.
[0031]3) Preparation of the inoculation hole: Select a...
Embodiment 3
[0035] Application of Fungus YNAS-8 to Induce Agarwood to Produce Agarwood
[0036] (1) The making of solid culture medium: preparation fructose concentration is 0.5%, and maltose concentration is 1%, and calcium sulfate concentration is the suspension of 1%; These 3 kinds of materials of buckwheat husk, wheat bran and corn flour are by volume ratio Mix evenly at a ratio of 1:3:0.1; add 1.6 liters of suspension per kilogram of dry material, and stir evenly. After aliquoting the medium, it was sterilized at 121°C for 180 minutes.
[0037] (2) Preparation of strains: Pick the strain block at the edge of the YNAS-8 colony activated on the PDA plate, inoculate it into the canned bottle with the above-mentioned solid substrate, and culture it in dark at 20-22° C. for 5 days. The vigorously growing fungal hyphae block was transferred to the can bottle equipped with buckwheat husk culture medium again, cultivated in dark at 20-22° C. for 10 days, and set aside.
[0038] (3) prepara...
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