Three fungi capable of inducing aquilaria sinensis to produce agilawood

A technology of white wood, fungus, applied in the field of bioengineering

Active Publication Date: 2012-08-29
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The three fungi were preserved in the General Microorganism Center of the C

Method used

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  • Three fungi capable of inducing aquilaria sinensis to produce agilawood

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Experimental program
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Effect test

Embodiment 1

[0021] Application of Fungus YNAS-4 to Induce Agarwood to Produce Agarwood

[0022] (1) Preparation of solid medium: prepare a suspension with 0.5% concentration of sucrose and calcium sulfate; mix the three materials of buckwheat husk, wheat bran and corn flour in a volume ratio of 1:1:0.05 ; Add 1 liter of suspension per kilogram of dry material and stir evenly. After the medium was dispensed, it was sterilized at 121°C for 165 minutes.

[0023] (2) Preparation of strains: Pick the strain block at the edge of the YNAS-4 colony activated on the PDA plate, inoculate it into a canned bottle equipped with the above-mentioned solid substrate, and culture in dark at 22-24° C. for 15 days. The vigorously growing fungal hyphae block was transferred to the canned bottle with buckwheat husk culture medium again, cultivated in dark at 22-24° C. for 30 days, and set aside.

[0024] (3) Preparation of the inoculation hole: select the white wood incense tree with a tree age of 5 years a...

Embodiment 2

[0028] Application of Fungus YNAS-6 to Induce Agarwood to Produce Agarwood

[0029] (1) The making of solid culture medium: preparation glucose concentration is 1.2%, and calcium sulfate concentration is the suspension of 1%; These 3 kinds of materials of buckwheat husk, wheat bran and corn flour are 2: 1.5: 0.07 by volume ratio The ratio is mixed evenly; add 0.8 liters of suspension per kilogram of dry material, and stir evenly. After aliquoting the medium, it was sterilized at 121°C for 140 minutes.

[0030] (2) Preparation of strains: Pick the strain block at the edge of the YNAS-6 colony activated on the PDA plate, inoculate it into the canned bottle with the above-mentioned solid substrate, and culture in dark at 25-28° C. for 15 days. The vigorously growing fungal hyphae block was transferred to the can bottle equipped with buckwheat husk medium again, and cultured in dark at 25-28° C. for 20 days, and set aside.

[0031]3) Preparation of the inoculation hole: Select a...

Embodiment 3

[0035] Application of Fungus YNAS-8 to Induce Agarwood to Produce Agarwood

[0036] (1) The making of solid culture medium: preparation fructose concentration is 0.5%, and maltose concentration is 1%, and calcium sulfate concentration is the suspension of 1%; These 3 kinds of materials of buckwheat husk, wheat bran and corn flour are by volume ratio Mix evenly at a ratio of 1:3:0.1; add 1.6 liters of suspension per kilogram of dry material, and stir evenly. After aliquoting the medium, it was sterilized at 121°C for 180 minutes.

[0037] (2) Preparation of strains: Pick the strain block at the edge of the YNAS-8 colony activated on the PDA plate, inoculate it into the canned bottle with the above-mentioned solid substrate, and culture it in dark at 20-22° C. for 5 days. The vigorously growing fungal hyphae block was transferred to the can bottle equipped with buckwheat husk culture medium again, cultivated in dark at 20-22° C. for 10 days, and set aside.

[0038] (3) prepara...

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Abstract

The invention discloses three fungi, which are respectively a Microsphaerosis sp. fungus YNAS-4 with the preservation No. of CGMCC No.4453, an Xylaria sp. fungus YNAS-6 with the preservation No. of CGMCC No.4455, and a Lasiodiplodia sp. fungus YNAS-8 with the preservation No. of CGMCC No.4456. The fungi can be inoculated onto a trunk or limb of aquilaria sinensis to induce the aquilaria sinensis to produce agilawood, and the fungi have wide application prospects in the aspects of improving the yield of the traditional Chinese medicine of agilawood, protecting the natural resource of aquilariasinensis and the like.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to three strains of fungi, which can be inoculated on the trunk or branch of arborica to induce agarwood to produce agarwood. Background technique [0002] The 2010 edition of "Chinese Pharmacopoeia" records that the base plant of the traditional Chinese medicine agarwood is the resinous wood of A. sinensis (Lour.) Gilg., a plant of the genus Aquilaria in the family Thymelaeceae. [0003] In the history of Chinese medicine in my country, the medicinal value of agarwood has been known for a long time. Li Shizhen recorded in "Compendium of Materia Medica": "Agarwood is pungent in nature, slightly warm, non-toxic, has the effect of lowering qi, regulating the middle, and clearing the liver. For: promoting qi and relieving pain, warming middle-JIAO to stop vomiting, invigorating qi and relieving asthma. It is used for distension and pain in the chest and abdomen, vom...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 郭顺星崔晋龙陈晓梅王春兰孟志霞
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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