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Geobacillus sp. UTM01 and application thereof

A technology of Geobacillus and bacteria agent, applied to the thermophilic Geobacillus strain UTM01 and its application field, can solve the problems of not seeing Geobacillus, achieve important economic value and application prospects, and promote the effect of composting process

Active Publication Date: 2012-09-12
北京绿源科创环境技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of Geobacillus as an inoculant in high-temperature sludge composting

Method used

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  • Geobacillus sp. UTM01 and application thereof
  • Geobacillus sp. UTM01 and application thereof
  • Geobacillus sp. UTM01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and identification of Geobacillus thermophile UTM01

[0030] In a sterile environment, weigh 10g of the sludge decomposed compost sample into a 100ml sterile water triangular flask with 10 glass beads, seal it with tin foil, place it on a shaker and vibrate at 160r / min for 30 minutes to make the sample fully Spread out, and then place it at 70°C for static enrichment culture for 6-12 days.

[0031] Take out the above enrichment culture triangular flask, use a sterile pipette to draw 1ml of the supernatant and add it to 9ml of sterile water to obtain (10 -1 )Diluent. And so on, make 10 -1 to 10 -3 Diluent. Take 9 sterile plates, use a 1ml sterile pipette to absorb 0.1ml of the above dilution solution, insert them into the plate, and set 3 replicates for each dilution; pour the LB medium that has been melted and cooled to about 50°C into the sterile In the petri dish, about 20ml per dish, fully mix with the bacterial solution, after solidification...

Embodiment 2

[0035] The preparation of embodiment 2 compost inoculants

[0036] (1) Activation culture: take the 4°C preserved strain of Geobacillus thermophilic strain UTM01 of the present invention, inoculate it on fresh LB solid medium, and activate and cultivate it at 60°C for 48 hours;

[0037] (2) Seed solution preparation: Inoculate an Erlenmeyer flask on the inclined plane of a test tube, inoculate all the bacterial colonies obtained from the activation culture into the Erlenmeyer flask containing LB culture solution, seal the bottle mouth, and place it on a water bath constant temperature oscillator , cultured with shaking at 65°C for 2 days at a shaking frequency of 160rpm;

[0038] (3) Preparation of fermented liquid: the shake flask seed liquid is inserted into a 100 L fermenter with 60 L of sterilized LB culture liquid by 5% (v / v), controlled at a temperature of 65° C., and ventilated for 20 minutes every 6 hours , the ventilation rate is 1:1 (v / v), stirred for 15 minutes every...

Embodiment 3

[0040] The preparation of embodiment 3 compost inoculants

[0041] (1) Activation culture: take the 4°C preserved strain of Geobacillus thermophilic strain UTM01 of the present invention, inoculate it on fresh LB solid medium, and activate it at 70°C for 24 hours;

[0042] (2) Seed liquid preparation: Inoculate an Erlenmeyer flask with an inclined test tube, inoculate the activated strain into an Erlenmeyer flask filled with sterilized LB culture medium, then seal the bottle mouth and place it in a water bath for constant temperature oscillation. Shake culture at 70°C for 36 hours on a shaker with a shaking frequency of 200rpm;

[0043] (3) Preparation of fermented liquid: the shake flask seed liquid is inserted into a 100 L fermenter with 60 L of sterilized LB culture liquid by 10% (v / v), controlled at a temperature of 70°C, and ventilated for 30 minutes every 4 hours , the ventilation rate was 1.5:1 (v / v), stirred for 10 minutes every 12 hours, stirred at 180rpm, and cultiv...

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Abstract

The invention provides a geobacillus sp. strain UTM01. The storage number of the geobacillus sp. strain UTM01 is CGMCC No.5641. The highest growth temperature of the geobacillus sp. strain UTM01 reaches 75 DEG C, the geobacillus sp. Strain UTM01 can rapidly multiply in a high temperature compost environment, organic matters are degraded, and a compost process is promoted. The invention further provides solid compost inoculant prepared from the geobacillus sp. strain UTM01, and a preparation method and application of the solid compost inoculant. The compost inoculant is low in production cost and stable in quality, can be applied to high-temperature sludge composting, does not affect moisture content of compost bodies, is environment-friendly and pollution-free, has excellent performances,and has good economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of environmental microbes, in particular to a thermophilic Geobacillus strain UTM01 and its application. Background technique [0002] Composting is an effective way to convert organic solid waste into simple organic matter and nutrients such as nitrogen. It has been widely concerned due to its economy and ease of operation, which provides an opportunity for the industrialization of composting. However, the development of industrialization of composting has always been Plagued by the cost of composting time and space. [0003] Compost inoculation starts from the composting process, which is a microorganism-led degradation and transformation mechanism of organic matter, isolates and domesticates dominant microbial strains with different functional roles from diverse environments or materials, and forms bacterial agents through culture and fermentation, and then inoculates them into compost To strengthen this...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C12R1/01
CPCY02W30/40
Inventor 刘永跃周顺桂许宜北汪涌
Owner 北京绿源科创环境技术有限公司
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