Confining liquid for enzyme linked immunosorbent assay in vitro diagnostic reagent

An in vitro diagnosis and enzyme-linked immunosorbent technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of short validity period of the kit, short validity period, and poor stability of the sealing reagent, so as to improve the stability and maintain the activity of the enzyme , the effect of maintaining protein stability

Inactive Publication Date: 2012-09-12
AILEX TECH GRP CO LTD
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AI Technical Summary

Problems solved by technology

The single-component blocking reagent has poor stability and a short validity period, which to a certain extent makes the kit's validity period very short

Method used

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  • Confining liquid for enzyme linked immunosorbent assay in vitro diagnostic reagent

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Embodiment

[0021] The preparation steps of blocking solution of the present invention are as follows:

[0022] A) prepare 0.01M~0.2M PBS buffer solution, the pH value is 6.5~7.8;

[0023] B) Add bovine serum albumin to the buffer solution in step A, the final concentration is 1g / L-30g / L, stir to make it fully dissolved;

[0024] C) adding trehalose, the final concentration is 0.2g / L~25g / L, stirring to make it fully dissolved;

[0025] D) Add protein stabilizer, the final concentration is 0.2g / L~5g / L, stir to make it fully dissolved;

[0026] E) Add L-lysine, the final concentration is 0.2g / L~10g / L, stir to make it fully dissolved;

[0027] F) Add Proclin 300, the final concentration is 0.01% ~ 1%, stir to make it fully mixed;

[0028] G) Adjust the pH value to 6.5-7.4 with 5M sodium hydroxide solution or 5M hydrochloric acid solution, and mix well;

[0029] H) Stand at room temperature for more than 30 minutes, and store at 2°C to 8°C.

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Abstract

The invention belongs to the technical field of biological in vitro diagnostic reagents and particularly relates to a confining liquid for an enzyme linked immunosorbent assay in vitro diagnostic reagent. The confining liquid is prepared by the following technological steps: preparing PBS (Phosphate Buffer Solution); adding albumin bovine serum to be dissolved fully; adding trehalose to be dissolved fully; adding protein stabilizing agent to be dissolved fully, adding L-lysine to be dissolved fully; adding Proclin 300 to be mixed fully; adding sodium hydroxide solution or hydrochloric acid solution for adjusting the pH value to be mixed fully; and standing for more than 30 min at room temperature and preserving at 2-8 DEG C. Compared with the prior art, the confining liquid has a broad spectrum activity, can inhibit the growth of microbe like bacteria, fungi and microzyme for a longer time, and meanwhile can retain the activity of enzyme in the system.

Description

[technical field] [0001] The invention belongs to the technical field of biological in vitro diagnostic reagents, in particular to a blocking solution in an enzyme-linked immunological in vitro diagnostic reagent. [Background technique] [0002] The basis of ELISA is the immobilization of antigen or antibody and the enzyme labeling of antigen or antibody. The basic principles are as follows: ①Antigen or antibody can be physically adsorbed on the surface of a solid phase carrier and maintain its immunological activity; ②Antigen or antibody can be The enzyme conjugate is formed by linking with the enzyme through a covalent bond, and the enzyme conjugate can still maintain its immunological and enzymatic activity; ③ After the enzyme conjugate is combined with the corresponding antigen or antibody, it can be detected according to the color reaction of the added substrate. Determine whether there is an immune reaction, and the depth of the color reaction is proportional to the am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/545
Inventor 李子樵李大军
Owner AILEX TECH GRP CO LTD
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