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Method for inducing polyploid of momordica grosvenori

A technology of Luo Han Guo and polyploidy, applied in the field of plant genetics and breeding, can solve the problems of different plant characteristics, differences in effects, no research and reports on Oryzalin Luo Han Guo polyploidy, etc.

Inactive Publication Date: 2012-09-19
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different plants have different characteristics, and the methods and effects of Oryzalin treatment are also very different.
[0004] There are no studies and reports on Oryzalin inducing polyploidy in Luo Han Guo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In the field, select the female plant of Luo Han Guo variety type "Qing Pi Guo" that has no occurrence of diseases and insect pests and grows robustly as the induced plant.

[0018] Entering the flowering period, the first day when the female flower is open, the pollen of the open male flower is used for pollination.

[0019] 24 hours after the pollination of the female flowers of Luo Han Guo, put the female flowers into a centrifuge tube filled with a 30 μM Oryzalin aqueous solution and soak for 24 hours.

[0020] After the treatment, take out the female flowers and wash them with water to remove Oryzalin.

[0021] Harvesting and processing fruit: when the female flower expands to form a fruit and the fruit color changes from green to yellow-green, pick the fruit. Compared with the control, after the treatment, the female flowers swelled normally, and the rate of fruit formation decreased significantly. After treatment 24 hours after pollination, the fruit maturit...

Embodiment 2

[0025] In the field, select the female plant of Luo Han Guo variety type "Qing Pi Guo" that has no occurrence of diseases and insect pests and grows robustly as the induced plant.

[0026] Entering the flowering period, the first day when the female flower is open, the pollen of the open male flower is used for pollination.

[0027] Forty-eight hours after the pollination of the female flowers of Luo Han Guo, the female flowers were placed in a centrifuge tube filled with 30 μM Oryzalin aqueous solution and soaked for 24 hours.

[0028] After the treatment, take out the female flowers and wash them with water to remove Oryzalin.

[0029] Harvesting and processing fruit: when the female flower expands to form a fruit and the fruit color changes from green to yellow-green, pick the fruit. Compared with the control, after the treatment, the female flowers expanded normally, and the rate of fruit formation decreased significantly. After 48 hours of treatment, the fruit maturi...

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PUM

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Abstract

The invention discloses a method for inducing a polyploid of momordica grosvenori. The method comprises the steps of dipping a pollinated momordica grosvenori female flower by using Oryzalin; and then washing and relieving the dipped flower; and inducing doubling of zygotes by using a novel chemical inducer Oryzalin so as to obtain the polyploid. According to the invention, the chimera problem of the polyploid of the momordica grosvenori is solved, the inducing operation procedures are simplified, the inducing efficiency of the polyploid of the momordica grosvenori is increased, and convenience is obtained in actual applications.

Description

technical field [0001] The invention belongs to the field of plant genetics and breeding, and relates to a method for inducing polyploidy in plants, in particular to a method for inducing polyploidy in Luo Han Guo. Background technique [0002] Polyploid refers to plants whose somatic cells have more than two chromosome sets. Since the discovery in 1937 that colchicine (colchicine) can induce chromosome doubling, people began to study plant polyploid induction. At present, using colchicine to induce autotetraploid in vitro is the main way to obtain polyploid plants. In the breeding of Luo Han Guo (Siraitia grosvenorii) of the family Cucurbitaceae, Jiang Shuiyuan and others also mainly used a certain concentration of colchicine to treat isolated tissue cultured seedlings to obtain polyploids (seedless Luo Han Guo breeding A preliminary study of [J]. Guangxi Plants, 2009, 29(4):506-509). However, colchicine treatment is highly toxic; in vitro treatment requires high tissue ...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 董志渊马小军缪剑华白隆华莫长明潘丽梅冯世鑫
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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