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Method for extracting total saponins from allium chinense

A technology for total saponins and rhizomes, which is applied in the field of extracting total saponins from rhizomes, can solve problems such as difficulties, complex plant active substances, and achieve the effects of easy availability, low price, and simple operating conditions.

Inactive Publication Date: 2013-11-27
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complex composition of plant active substances, they often contain certain impurities such as volatile oils, polysaccharides, flavonoids, and alkaloids, and the properties of the substances are also very different. Therefore, there are certain difficulties in obtaining high-purity saponins, which need to be removed through multiple steps. Impurities

Method used

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  • Method for extracting total saponins from allium chinense
  • Method for extracting total saponins from allium chinense
  • Method for extracting total saponins from allium chinense

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Embodiment Construction

[0027] The present invention is described in further detail below in conjunction with embodiment.

[0028] This embodiment includes the following steps:

[0029] (1) Raw material pretreatment: take fresh onion, wash it with tap water, then wash it twice with distilled water, dry it, and remove the water in the onion;

[0030] (2) Oven drying: place the onion treated in step (1) in an oven at 50°C and continue drying for 12 hours until the moisture content is ≤8%; while removing excess water in the onion, remove the Sulfur-containing compounds to improve the purity of the extract;

[0031] (3) crushing the onion bulbs dried in step (2) through a pulverizer and passing through a 200-mesh sieve, removing uncrushed onion bulbs, and collecting onion powder;

[0032] (4) Preliminary extraction by powder immersion: Weigh 10g of onion powder and place it in a glass container, then add 80g of ethanol solution with a volume concentration of 40% of the product into the glass container,...

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Abstract

The invention discloses a method for extracting total saponins from allium chinense. The method comprises the following steps of: (1) pretreating raw materials; (2) drying in an oven; (3) crushing; (4) soaking the powder and performing primary extraction; (5) performing secondary extraction; (6) evaporating and recycling ethanol, and performing reduced-pressure concentration; (7) extracting with petroleum ether; (8) extracting with n-butyl alcohol; (9) evaporating and recycling the n-butyl alcohol, and concentrating with a concentrator; (10) performing column purification with macroporous adsorption resin; and (11) removing the ethanol and drying. In the invention, the raw materials are easily available at low price; all reagents are in common use and can be recycled to lower the production cost and reduce pollution; the operation conditions are simple and easy to realize; the obtained product contains relatively high content of total saponins of allium chinense, and has high values in practical application; and the residues after the extraction of total saponins also can be used as high-quality feed and fertilizer.

Description

technical field [0001] The invention relates to a method for extracting total saponins, in particular to a method for extracting total saponins from onion heads. Background technique [0002] Onion head, also known as buckwheat head ( Allium chines G. Don. ), small garlic ( Allium Macrostemon Bunge ), etc., are the underground bulbs of the perennial herb Liliaceae Allium. Onion contains a variety of biologically active substances, such as polysaccharides, proteins, calcium, phosphorus, iron, carotene, vitamin C, flavonoids and saponins, etc. Dried scallions can be used as medicine to invigorate the stomach, clear phlegm, and treat chronic gastritis. According to the records of "The Classic of Classics": "Helicopterus" can be seen "to cure gold sores and sores, and those who are light-weight will not be hungry and resistant to old age", "to treat Shaoyin disease, dysentery and dysentery, and chest pain, qi, blood and miscarriage" can be seen The medical application of sc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8962A61P3/06A61P3/10A61P9/10A61P31/00A61P31/12A61P35/00A61P37/02
Inventor 丁学知夏立秋禹智辉孙运军胡胜标余子全肖秀情
Owner HUNAN NORMAL UNIVERSITY
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