High-emulation tissue-engineered nerve repair material
A technology for nerve repair and tissue simulation, applied in medical science, prostheses, etc., can solve problems such as the inability to maintain the physical structure, affect the effect of treatment or experiment, and unfavorable directional growth of regenerative fibers, so as to facilitate regeneration and improve mechanical properties , Increase the effect of tensile strength and stiffness
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Embodiment 1
[0035] Example 1: (according to the ratio of type I collagen: chitosan: gelatin is 280:70:70):
[0036] According to technical scheme of the present invention, taking collagen protein, gelatin and chitosan as example, its preparation method is carried out as follows:
[0037] 1). Take the type I collagen of the above ratio by weight parts, put it into 3 mg / ml acetic acid solution to dissolve for 24 hours according to the concentration of 28 mg / ml;
[0038] 2). Stir at 15000r / min for 90min in a constant temperature environment at 4°C to make a suspension;
[0039] 3). In addition, take chitosan and gelatin in the above weight ratio according to the ratio and add them to 3 mg / ml acetic acid solution to dissolve for 24 hours;
[0040] 4). Constant temperature at 4°C, stirring at 15000r / min for 90min to make a suspension;
[0041] 5). Mix the two suspensions and keep the constant temperature at 4°C, stir at 15000r / min for 90min to make a suspension of collagen and chitosa...
Embodiment 2
[0049] Example 2 : Adopt collagen (type I), chitosan and gelatin as raw materials, and its weight is 280 mg, 280 mg, 280 mg respectively, and the implementation process is the same as in Example 1.
Embodiment 3
[0050] Example 3: Collagen (type I), chitosan and gelatin were used as raw materials, the weights of which were 280 mg, 28 mg and 28 mg respectively, and the implementation process was the same as in Example 1.
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