High-emulation tissue-engineered nerve repair material

A technology for simulating tissue and nerve repair, applied in the field of high-simulation tissue engineering nerve repair materials, can solve the problems of unable to maintain the physical structure, affect the effect of treatment or experiment, and unfavorable directional growth of regenerative fibers, so as to facilitate cell migration and nutrition Effects of material transport and exchange, uniform internal pore size, and axially parallel arrangement height simulation

Active Publication Date: 2014-04-16
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the internal structure of the previous composite bridging carrier materials has no certain rules (in terms of pore size and direction), the ideal physical structure cannot be maintained in vivo, which is not conducive to the directional growth of regenerated fibers in spinal cord or peripheral nerve segmental injuries, thus affecting The effect of a treatment or experiment
Preparation of collagen-chitosan bridging materials with simulated structural features of axis-oriented microtubule matrix arrangement, there is no similar report in the world

Method used

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  • High-emulation tissue-engineered nerve repair material
  • High-emulation tissue-engineered nerve repair material
  • High-emulation tissue-engineered nerve repair material

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0035] Example 1: (according to the ratio of type I collagen: chitosan: gelatin is 280:70:70):

[0036] According to technical scheme of the present invention, taking collagen protein, gelatin and chitosan as example, its preparation method is carried out as follows:

[0037] 1). Weigh the above-mentioned type I collagen in parts by weight, and dissolve it in 3 mg / ml acetic acid solution at a concentration of 28 mg / ml for 24 hours;

[0038] 2). Stir at 15000r / min for 90min in a constant temperature environment at 4°C to make a suspension;

[0039] 3). In addition, weigh chitosan and gelatin according to the above weight ratio and add them to 3 mg / ml acetic acid solution to dissolve for 24 hours;

[0040] 4). At a constant temperature of 4°C, stir at 15000r / min for 90min to make a suspension;

[0041] 5). Mix the two suspensions and keep the temperature at 4°C, stir at 15000r / min for 90 minutes to make a suspension of collagen and chitosan, and leave it for 12 hours under vac...

Embodiment 2

[0049] Embodiment 2: Collagen (type I), chitosan and gelatin are used as raw materials, the weights of which are 280 mg, 280 mg and 280 mg respectively, and the implementation process is the same as that of Example 1.

Embodiment 3

[0050] Embodiment 3: Collagen (type I), chitosan and gelatin are used as raw materials, the weights of which are 280 mg, 28 mg and 28 mg respectively, and the implementation process is the same as that of Example 1.

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Abstract

The invention relates to a high-emulation tissue-engineered nerve repair material which is characterized by being prepared from mixing one, two or three of type I collagen, chitosan and gelatin. The material is prepared from the following raw materials in parts by weight: 1-10 parts of the type I collagen and 1 part of the chitosan. The material can be used for the basic research on nerve injury repair and the bridge repair of clinic human spinal cord and peripheral nerve injury or defect, is beneficial to the growth of nerve regenerated fiber and can also be used for the repair of spinal cord and peripheral nerve injury.

Description

technical field [0001] The invention relates to multiple biological raw materials such as collagen, gelatin and chitosan, as well as their processing technology and production method, and is specifically a highly simulated tissue engineering nerve repair material. Background technique [0002] The repair of nerve damage is a worldwide problem. In recent years, the application of tissue engineering technology to prepare repair scaffold materials and assemble tissue engineering substitutes to repair nerve injuries has become a research hotspot. Compatibility and degradability studies; (2) Combined application of scaffolds, extracellular matrix and neurotrophic factors; (3) Introduction of seed cells. Among them, the scaffold is used as the carrier of seed cells and neurotrophic factors, and as the main body of the microenvironment for repairing nerve damage. The research on its construction and performance improvement has become the key to restricting the repair of peripheral...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/22A61L27/20
Inventor 胡学昱罗卓荆黄景辉
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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