Mycoplasma culture identification and medicine sensitive detection plate and method for identifying truth of result of C+ hole

A mycoplasma, true and false technology, applied in the field of clinical medical testing and in vitro diagnosis, can solve the problems of ergoenol depletion, death, fungal cell membrane rupture, etc.

Inactive Publication Date: 2012-09-19
武汉菁华时间科技有限公司
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

[0009] Antifungal drugs, such as azoles or alkylamines, mainly inhibit fungal cytochrome P 450 (CYP)14α-demethylase, or inhibit squalene cyclooxygenase, which leads to depletion of ergoenol, rupture and death of fungal cell membrane, so it is highly sensitive to fungi, but has little effect on mycoplasma

Method used

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  • Mycoplasma culture identification and medicine sensitive detection plate and method for identifying truth of result of C+ hole
  • Mycoplasma culture identification and medicine sensitive detection plate and method for identifying truth of result of C+ hole

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Embodiment Construction

[0034]The media storage area (1) is a large circular hole with a diameter large enough to accommodate reagent vials. The control area (2) is two holes up and down, the upper one is the C+ small hole, and the lower one is the C-hole. The identification area (3) is two parallel wells, respectively coated with amoxicillin (AMO) and fluconazole (FLC). The drug susceptibility test area (4) is two rows of 16 wells, respectively coated with acetylspiramycin (ACE), roxithromycin (ROX), azithromycin (AZI), clindamycin (CLI), doxycycline (DOX), levofloxacin (LEV), gatifloxacin (GAT), and pazufloxacin (PAZ) are eight antibiotics in total, the upper row is the high concentration group, and the lower row is the low concentration group.

[0035] The following describes the operation method of the drug susceptibility test panel in conjunction with the Mycoplasma pneumoniae rapid test kit (6-hour culture method) produced by our company.

[0036] When taking the reagents in the Mycoplasma pn...

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Abstract

The invention discloses a mycoplasma medicine sensitive test plate, which is provided with an identification area on the basis of the common medicine sensitive test plate and can identify the truth of a result of a C+ hole while performing mycoplasma culture and medicine sensitive tests. The invention also discloses a method for identifying the truth of the result of a mycoplasma positive control hole (C+ hole). According to the method, a culture result property identifying area is increased on the basis of the mycoplasma medicine sensitive test plate, a broad-spectrum antibiotic medicine of non anti-mycoplasma microorganisms is enveloped in the identification area, and sundry bacteria which easily cause false positive and false negative in the positive control hole (C+ hole) are inhibited from growing or directly inactivated, so that a culture result is identified; and the medicine enveloped can be one or more of beta-lactam medicines, sulfanilamide medicines, rifamycins, vancomycin, antifungal medicines and the like.

Description

technical field [0001] The present invention relates to the technical fields of clinical medical examination and in vitro diagnosis. Concretely, it is a kind of drug susceptibility test board. This drug susceptibility test board is on the basis of common drug susceptibility test board. + Hole results for authenticity identification. The specific identification method is to increase the identification area of ​​the nature of the culture results on the basis of the mycoplasma drug susceptibility test plate, and the identification area is coated with broad-spectrum antibiotics that are not anti-mycoplasma microorganisms, and the positive control well (C + Inhibition of growth or direct inactivation of miscellaneous bacteria that are likely to cause false positives and false negatives in the holes) so as to achieve the identification effect on the culture results. The specific coated drugs can be: β-lactam drugs, sulfonamide drugs, One or more of foomycin, vancomycin and antifun...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/18C12R1/35
Inventor 岳力群杨会宣
Owner 武汉菁华时间科技有限公司
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