Process for extracting protein of cordyceps militaris, and optimization method for optimal extraction conditions of process

An extraction process and an optimal extraction technology, applied in the field of health food preparation, can solve the problems of rare health products, less research and utilization of Cordyceps militaris protein, etc.

Inactive Publication Date: 2012-09-26
惠州市鑫福来实业发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For these health products developed for functional ingredients such as cordycepin, cordyceps polysaccharide, and adenosine, after extracting cordycepin, cordyceps polysaccharide, and adenosine with water or edible alcohol during production, the rest is discarded. It is seldom used, and related health products developed by using it are even rarer in the market

Method used

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  • Process for extracting protein of cordyceps militaris, and optimization method for optimal extraction conditions of process
  • Process for extracting protein of cordyceps militaris, and optimization method for optimal extraction conditions of process
  • Process for extracting protein of cordyceps militaris, and optimization method for optimal extraction conditions of process

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] After crushing the fruiting body of Cordyceps militaris, sieve 80 mesh and weigh 8g, add 120mL of 0.1mol / L sodium hydroxide solution, and extract at 50°C for 120min. After the reaction, centrifuge for 30 minutes at a speed of 3500 r / min to obtain a filtrate.

[0043] Adjust the pH of the obtained filtrate to the isoelectric point of the Cordyceps militaris protein of 4.8 with hydrochloric acid solution, centrifuge for 20 minutes at a speed of 3000 r / min to obtain a precipitate, wash with distilled water and vacuum dry to obtain the Cordyceps militaris protein.

Embodiment 2

[0045] After crushing the fruiting body of Cordyceps militaris, 10 g was weighed through a 80-mesh sieve, added with 200 mL of 0.15 mol / L sodium hydroxide solution, and extracted at 60° C. for 90 min. After the reaction, centrifuge for 30 minutes at a speed of 3500 r / min to obtain a filtrate.

[0046] Adjust the pH of the obtained filtrate to the isoelectric point of the Cordyceps militaris protein of 4.8 with hydrochloric acid solution, centrifuge for 20 minutes at a speed of 3000 r / min to obtain a precipitate, wash with distilled water and vacuum dry to obtain the Cordyceps militaris protein.

Embodiment 3

[0048] After crushing the fruiting body of Cordyceps militaris, sieve 80 meshes, weigh 15 g, add 150 mL of 0.05 mol / L sodium hydroxide solution, and extract at 70° C. for 150 min. After the reaction, centrifuge for 30 minutes at a speed of 3500 r / min to obtain a filtrate.

[0049] Adjust the pH of the obtained filtrate to the isoelectric point of the Cordyceps militaris protein of 4.8 with hydrochloric acid solution, centrifuge for 20 minutes at a speed of 3000 r / min to obtain a precipitate, wash with distilled water and vacuum dry to obtain the Cordyceps militaris protein.

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Abstract

The invention discloses a process for extracting the protein of cordyceps militaris and an optimization method for optimal extraction conditions of the process. According to the process, the sporocarp of cordyceps militaris, which is used as a main raw material, is crushed into powder, then the powder is screened with a sieve, and the protein is extracted by an alkali extraction and acid precipitation method. The extraction conditions are optimized by single-factor experiment and response surface methodology so as to obtain the optimal extraction process. According to the method, the process is simple, cost is low, and the raw materials are wide in source. The cordyceps militaris is rich in protein, and a large amount of research on the process for extracting the protein is not provided at present; and the method provides practice guidelines for the relative large-scale production of protein and the development of relative products, and has potential market development prospect.

Description

[0001] technical field [0002] The invention relates to a process for extracting protein from Cordyceps militaris and an optimization method for the best extraction conditions, belonging to the field of health food preparation. Background technique [0003] Cordyceps militaris, also known as Cordyceps militaris, Chrysalis militaris, etc., is a new resource food announced by the Ministry of Health. It belongs to Eumycota, Ascomycotinia, and Sclerotinia in taxonomy. (Pyrenomycetes), Sphaeriales, Claviciptiaceae, Cordycps. Cordyceps sinensis, ginseng, and velvet antler are the three traditional Chinese tonics and are well-known both at home and abroad. The medicinal chemistry, pharmacology and clinical experiments have proved that Cordyceps militaris can be used as a substitute for Cordyceps sinensis. Traditional Chinese medicine believes that Cordyceps enters the two meridians of the lung and kidney, which can not only nourish the lung yin, but also nourish the kidney yang. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
Inventor 陈小明李晨曦范青生余宙姜兴华刘剑青
Owner 惠州市鑫福来实业发展有限公司
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