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Swine immune trait correlated EHMT2 gene fragment cloning and application as molecular marker

A technology assisted by gene fragments and molecular markers, applied in the cloning and application of molecular markers, can solve the problems of no research on porcine EHMT2 gene molecular markers, no reports of porcine EHMT2 gene polymorphisms, etc.

Inactive Publication Date: 2012-09-26
HUAZHONG AGRI UNIV
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Problems solved by technology

[0005] However, at present, the research on the EHMT2 gene at home and abroad is mainly focused on the biological functions of humans and mice, and the polymorphism of the pig EHMT2 gene has not been reported. So far, there has been no research on the molecular markers related to the pig EHMT2 gene and pig immunity.

Method used

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  • Swine immune trait correlated EHMT2 gene fragment cloning and application as molecular marker
  • Swine immune trait correlated EHMT2 gene fragment cloning and application as molecular marker
  • Swine immune trait correlated EHMT2 gene fragment cloning and application as molecular marker

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Embodiment 1

[0026] Embodiment 1: preparation embodiment

[0027] 1. Cloning of partial fragments of EHMT2 gene and screening of mutation sites

[0028] 1. Primer Design

[0029] Using the primer design software Primer 5.0, the pig EHMT2 gene sequence (GeneBank accession number: EU709015.1) was used as a template to design primers containing the 27th intron sequence, which were used to amplify "Du Dachang" (from the foreign variety "Du "Lock", "Longbai" and "Dabai" are obtained by crossing three breeds, and it is a kind of commercial pig widely used in China) The partial sequence of the 27th intron of the EHMT2 gene. The synthesis of the primer pair was completed by Shanghai Bioengineering Technology Service Co., Ltd. The DNA sequence of the primer pair is as follows:

[0030] Forward primer: 5′-gccttcttcagttcacgagac-3′,

[0031] Reverse primer: 5'-tctgggacatcaaaagcaaatat-3'.

[0032] 2. Purification and sequencing of PCR products

[0033] (1) PCR amplification

[0034] The PCR ampli...

Embodiment 2

[0067] Embodiment 2: application example (the application of the molecular marker of the present invention in the association analysis of pig immune traits)

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Abstract

The invention belongs to the technical field of livestock molecular marker preparation and specifically relates to the preparation of a swine immunity correlated molecular marker for swine marker-assisted selection and application and an application thereof. The molecular marker is obtained by swine EHMT2 gene cloning. The gene sequence is as shown in SEQ ID NO:1 in a sequence table. There is a base mutation of A302-C302 at 302bp of SEQ ID NO:1 in the sequence table. The invention also discloses a primer used in 27th intron sequence of the amplified EHMT2 gene and an A302-C302 molecular marker detection method. The invention provides a novel molecular marker for swine marker-assisted selection.

Description

technical field [0001] The invention belongs to the technical field of livestock genetic engineering, and in particular relates to the cloning and application of molecular markers related to pig immune traits used as pig marker-assisted selection. Background technique [0002] In recent years, due to the serious outbreak of various animal diseases worldwide, and the residues of drugs and their harmful substances affect animal food safety and the environment, more and more attention has been paid to animal disease resistance genetic breeding, which has become an important goal of animal breeding. Moreover, with the development of molecular biology and bioinformatics, it is possible to use molecular disease-resistant breeding technology to quickly and effectively breed new disease-resistant varieties, which has opened up a new way to improve animal disease resistance. Molecular marker-assisted selection is currently an effective disease-resistant breeding technology, and trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/11C12Q1/68
Inventor 张淑君杨利国刘文举赵书红任钰为童勤宋利军江一波刘向东熊家军滑国华周傲张杨
Owner HUAZHONG AGRI UNIV
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