CCD (charge coupled device) camera directly coupled with sequencing chip

Abstract

The invention discloses a CCD (charge coupled device) camera directly coupled with a sequencing chip. The CCD camera comprises a sequencing chip clamped in an installation base of a DNA (deoxyribonucleic acid) sequencer, a camera body and an optical fiber panel for catching optical signals of the sequencing reaction, wherein one end of the optical fiber panel extends out from the front end of thecamera body and is directly contacted one side of the sequencing chip located on the installation base of the sequencing chip of the DNA sequencer in order to obtain optical signals produced by sequencing reaction at the other side of the sequencing chip. With the adoption of the CCD camera directly coupled with the sequencing chip, due to the optical fiber panel directly coupled with the sequencing chip, the weak visible light produced on the sequencing chip can be directly received by the optical fiber panel to be converted into an electric signal; therefore, the optical coupling efficiencyis improved; the optical coupling efficiency can be more than 70%; and the condition that the high-quality sequencing signal is acquired can be ensured.

Description

technical field [0001] The invention relates to the technical field of DNA sequencing, in particular to a CCD camera directly coupled with a sequencing chip, so that an optical fiber panel can directly contact the sequencing chip, ensuring the acquisition of sequencing signals with a high signal-to-noise ratio. Background technique [0002] Pyrosequencing technology is an enzyme cascade chemiluminescent reaction in the same reaction system catalyzed by four enzymes. The principle of pyrosequencing technology is: after the primer anneals to the template DNA, the four enzymes DNA polymerase (DNA polymerase), ATP sulfurylase (ATP sulfurylase), luciferase (luciferase) and adenosine triphosphate (Apyrase) Under the synergistic effect of the primer, the polymerization of each dNTP on the primer is coupled with the release of an optical signal, and the purpose of real-time determination of DNA sequence is achieved by detecting the release and intensity of light. The reaction syste...

AI Technical Summary

Problems solved by technology

Since the sequencing signal is an extremely weak optical signal in the visible light band, due to the existence of the optical lens, the optical signal will cause a certain attenuation during the transmission process, which affects the intensity of the optical signal transmitted to the optical fiber panel

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