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Method for extracting glutamic acid decarboxylase from banana peel and method for producing r-reanal

A technology of glutamic acid decarboxylase and aminobutyric acid, applied in the direction of lyase, fermentation, etc., can solve the problems of long production cycle, high production cost, and difficult separation, and achieve low cost, convenient operation, and simple production process Effect

Inactive Publication Date: 2012-10-03
NANJING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of GAD from microbial cells and the production of GABA by microbial fermentation also have the disadvantages of long production cycle, difficult downstream separation, and high production cost.
However, there is currently no technology to extract food-grade GAD from food materials and use it to safely produce GABA

Method used

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  • Method for extracting glutamic acid decarboxylase from banana peel and method for producing r-reanal
  • Method for extracting glutamic acid decarboxylase from banana peel and method for producing r-reanal
  • Method for extracting glutamic acid decarboxylase from banana peel and method for producing r-reanal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Use a soybean milk machine to crush 1kg of banana peel until it becomes muddy, so that the fiber and protein are separated. The length of the fiber is 2mm. Put it in a conical flask and add 2L of vitamin B with a pH of 5.5 and 0.1g / L. 6 Phosphate buffer (Na 2 HPO 4 / NaH 2 PO 4 ), shake well, stand for extraction for 4 hours, then centrifuge at 9000 rpm for 10 minutes to obtain crude enzyme solution.

Embodiment 2

[0037] Use a soybean milk machine to crush 1kg of banana peel until it becomes muddy, so that the fiber and protein are separated. The length of the fiber is 2mm. Put it in a triangular flask, add 5L of vitamin B with a pH of 5.5 and 0.1g / L 6 Phosphate buffer (Na 2 HPO 4 / NaH 2 PO 4 ), shake well, stand for extraction for 4 hours, then centrifuge at 9000 rpm for 10 minutes to obtain crude enzyme solution.

Embodiment 3

[0039] Use a soybean milk machine to crush 1kg of banana peel until it becomes muddy, so that the fiber and protein are separated. The length of the fiber is about 2mm. Put it in a conical flask and add 7L of vitamin B with pH5.5 and 0.1g / L 6 Phosphate buffer (Na 2 HPO 4 / NaH 2 PO 4 ), shake well, stand for extraction for 4 hours, then centrifuge at 9000 rpm for 10 minutes to obtain crude enzyme solution.

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Abstract

The invention relates to a method for extracting glutamic acid decarboxylase from a banana peel, which comprises the steps of smashing the banana peel to paste to separate fiber and protein, wherein the length of fiber does not exceed 2mm; adding a phosphate buffer which is 5.0-7.0 in pH and contains 0.01-0.2g / L vitamin B6, shaking uniformly and standing and extracting for 1-5h, wherein each kilogram of banana peel corresponds to 2-7 liters of phosphate buffer; centrifugalizing at 6000-10000rpm for 10-50min to obtain a GAD (Glutamic Acid Decarboxylase) enzyme solution. The invention further provides a method for producing r-reanal by extracting glutamic acid decarboxylase from the banana peel. According to the invention, the waste in banana processing industry can be effectively used, the GAD and GABA (r-reanal) production costs are reduced, and the method can be used to produce GABA in a large scale.

Description

technical field [0001] The invention belongs to the field of production technology of food functional components, and relates to a method for extracting glutamic acid decarboxylase from banana peel and producing gamma-aminobutyric acid. Background technique [0002] γ-aminobutyric acid (GABA, also known as 4-aminobutyric acid, aminobutyric acid and piperidine acid, the molecular formula is HOOCCH 2 CH 2 CH 2 NH 2 , with a molecular weight of 103.12), is a non-protein functional amino acid that widely exists in nature and is an important inhibitory neurotransmitter in the central nervous system of mammals. It has important physiological functions, such as regulating blood pressure and heart rate, regulating Blood pressure, nutrition of nerve cells, treatment of epilepsy, promotion of mental stability, promotion of brain blood flow, promotion of brain vitality, promotion of growth hormone secretion, auxiliary treatment of asthma, activation of liver and kidney function, pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12P13/00
Inventor 缪冶炼王琲陈玲
Owner NANJING UNIV OF TECH
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