Function of mini-chromosome maintenance complex component in disease diagnosis and prevention of systemic lupus erythematosus

A minichromosome and lupus erythematosus technology, applied in allergic diseases, anti-animal/human immunoglobulins, antibodies, etc., can solve the problems of not knowing the specific role of minichromosome maintenance protein and not reporting the relationship between systemic lupus erythematosus

Inactive Publication Date: 2012-10-03
侯亚义 +2
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] It has been reported that the transcription of the MCM gene may be regulated by certain factors and play a role in autoimmune diseases. However, the specific role of minichromosome maintenance protein in autoimmune diseases is not known in the art, let alone its relationship with systemic erythema. lupus relationship

Method used

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  • Function of mini-chromosome maintenance complex component in disease diagnosis and prevention of systemic lupus erythematosus
  • Function of mini-chromosome maintenance complex component in disease diagnosis and prevention of systemic lupus erythematosus
  • Function of mini-chromosome maintenance complex component in disease diagnosis and prevention of systemic lupus erythematosus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1: The expression of minichromosome maintenance protein 6 in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus was significantly higher than that of normal people;

[0081] The present invention took blood samples from 22 SLE patients and 10 normal persons, separated PBMCs with lymphocyte separation fluid, then extracted total RNA with Trizol, reverse-transcribed it into cDNA with reverse transcriptase, and then used real-time quantitative PCR The level of MCM6 mRNA was detected. It was found that the mRNA level of MCM6 in PBMCs of SLE patients was significantly higher than that of normal people ( figure 1 ).

Embodiment 2

[0082] Example 2: The expression of minichromosome maintenance protein 6 in dendritic cells (DCs) of patients with systemic lupus erythematosus was significantly higher than that of normal people;

[0083] The present invention took blood samples from 5 patients and 4 normal persons, separated PBMCs with lymphocyte separation fluid, and then isolated dendritic cells with magnetic beads, then extracted total RNA with Trizol, and used reverse transcriptase It was reverse transcribed into cDNA, and the mRNA level of MCM6 was detected by real-time quantitative PCR. The results showed that the expression level of MCM6 in dendritic cells of SLE patients was also significantly higher than that of normal people ( figure 2 ).

Embodiment 3

[0084] Example 3: The expression level of minichromosome maintenance protein 6 in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus is positively correlated with the level of estrogen;

[0085] The present invention took blood samples from 11 cases of SLE patients, separated PBMCs with lymphocyte separation fluid, then extracted total RNA with Trizol, reverse-transcribed it into cDNA with reverse transcriptase, and then detected the mRNA of MCM6 by real-time quantitative PCR s level. At the same time, the Architect i2000SR system of Abbott Company was used to detect the level of estrogen in the patient's serum. The results showed that the expression level of minichromosome maintenance protein 6 in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus was positively correlated with the level of estrogen ( image 3 ).

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Abstract

The invention belongs to the field of biotechnology, and discloses a function of a mini-chromosome maintenance complex component which plays an important role in immune dysfunction of systemic lupus erythematosus (SLE). The invention also discloses a method for detecting the level of the mini-chromosome maintenance complex component, and then for the auxiliary diagnosis of the systemic lupus erythematosus. The function of the mini-chromosome maintenance complex component in disease diagnosis and prevention of the systemic lupus erythematosus for the first time demonstrates that the mini-chromosome maintenance complex component family is closely related to the systemic lupus erythematosus, and the regulation and intervention of the mini-chromosome maintain protein level can improve the function of immune cells, and also provides a new drug target for the prevention and treatment of systemic lupus erythematosus.

Description

Technical field: [0001] The invention belongs to the field of biotechnology; specifically, the invention discloses the important role of the minichromosome maintenance protein in systemic lupus erythematosus, clarifies that the minichromosome maintenance protein can be used as an auxiliary detection index of systemic lupus erythematosus, and reduces the microchromosome maintenance protein in systemic lupus erythematosus. The role in the prevention and treatment of systemic lupus erythematosus. Background technique: [0002] Systemic lupus erythematosus (SLE) is an autoimmune disease involving many systems and organs, and its clinical and immunological phenotypes are extremely complex and diverse. Systemic lupus erythematosus affects not only humoral immunity, but also cellular immunity, and changes in the complement system, which almost cover the entire immune system. Since the etiology and pathogenesis of systemic lupus erythematosus are still unclear, it also brings great...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C07K16/18C07K14/47A61K39/395A61P37/02
Inventor 侯亚义谢浩花春艳
Owner 侯亚义
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