Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for eliminating tissue culture seedling pollution

A technology for tissue culture and decontamination, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as secondary pollution, unsatisfactory sterilization effect, complicated operation, etc., and achieve convenient addition, great ecological benefits and Economic benefits, the effect of eliminating pollution

Inactive Publication Date: 2012-10-17
SHANDONG YANTAI AGRI SCHOOL
View PDF2 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for eliminating the contamination of tissue culture seedlings, so as to solve the problems of unsatisfactory sterilization effect, complicated operation, easy secondary pollution and no promotional value existing in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: Elimination of Pollution of Japanese Yamato Taro Tissue Cultured Plantlets

[0015] The tissue-cultured seedlings of Yamatota japonica were obtained by differentiation and culture at the top of growth. During the transfer process, due to improper operation or contamination of the tissue-cultured seedlings by endophytes, plaques appeared on the roots or on the surface of the culture medium. Transfer the polluted tissue culture seedlings to the medium containing sodium hypochlorite with a concentration of 5mg / L through normal transfer technology, so that the pH value of the medium is 5.5, and the culture conditions of the tissue culture seedlings: temperature 22°C , the light condition is 12 hours a day, the light intensity is 2000lx, and every 10 days is a cycle. After a cycle, according to the growth status of bacterial plaque and tissue culture seedlings, transfer the tissue culture seedlings to the medium containing new sodium hypochlorite solution , aft...

Embodiment 2

[0016] Embodiment 2: Elimination of Pollution of Hemerocallis grandiflora tissue culture seedlings

[0017] The polluted Hemerocallis grandiflora tissue culture seedlings are transferred to the medium containing sodium hypochlorite with a concentration of 20 mg / L through normal transfer technology, so that the pH value of the medium is 5.7, and the culture conditions of the tissue culture seedlings are: temperature 24°C, light conditions 12 hours a day, light intensity 2000lx, every 20 days as a cycle, after a cycle, according to the growth status of bacterial plaque and tissue culture seedlings, then transfer the tissue culture seedlings to the culture containing new sodium hypochlorite solution Basically, after 3 cycles in this way, the pollution is eliminated and robust tissue culture seedlings are obtained.

[0018] Implementation Example 3: Elimination of Pollution of Cut Chrysanthemum Tissue Cultured Plantlets

[0019] Transfer the polluted cut chrysanthemum tissue-cult...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for eliminating tissue culture seedling pollution, and the method is characterized by including the following steps of: (1) adding a sodium hypochlorite solution into a culture medium used for culturing tissue culture seedlings; (2) transferring a polluted tissue culture seedling to the sodium hypochlorite solution-containing culture medium; (3) repeating step (1) and (2), and conducting transfer several times so as to eliminate pollution; and (4) culturing the pollution eliminated tissue culture seedling. The method of the invention has the advantages that: compared with other antibiotics, the sodium hypochlorite solution adopted in the invention is convenient to add and can be subjected to autoclaving along with the culture medium; compared with mercuric chloride, the sodium hypochlorite solution has no secondary pollution, and tissue culture seedling pollution can be effectively eliminated; and the method can be used to save polluted tissue culture seedlings in tissue culture seedling species preservation and rapid propagation of seedlings for production, thus boasting substantial ecological and economic benefits.

Description

technical field [0001] The invention relates to a method for eliminating pollution of tissue culture seedlings, in particular to a method for eliminating pollution of tissue culture seedlings by using sodium hypochlorite. Background technique [0002] In the process of plant tissue culture, the pollution phenomenon is one of the three major problems in the current tissue culture process. Due to various reasons, bacterial contamination of the tissue culture seedlings occurs, and the polluted tissue culture seedlings are generally discarded, resulting in production The increase of cost and the delay of farming time often cause irreparable losses to the preservation of plant resources. At present, in tissue culture, antibiotics are used for sterilizing treatment, but so far no antibiotics have been found to be effective against all kinds of bacteria, and the effect is not ideal. Some use mercuric chloride for prevention and treatment, but after mercuric chloride is used, it is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 史淑一林丽丽辛秀琛张曰秋王红艳姜宁张志芬
Owner SHANDONG YANTAI AGRI SCHOOL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com