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Method for extracting and purifying Codonopsis pilosula polysaccharide

A technology of Codonopsis polysaccharides and Codonopsis pilosula, which is applied in the field of extracting and purifying Codonopsis polysaccharides, which can solve the problems of deep color, large damage to the structure and activity of Codonopsis polysaccharides, and achieve the effect of improving purity

Inactive Publication Date: 2012-10-17
谭智丽 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Codonopsis polysaccharide extracts sold in the market often have darker colors due to excessive pigment impurities. Although some Codonopsis polysaccharide extracts are lighter in color, they are often decolorized by oxidation, which greatly damages the structure and activity of Codonopsis polysaccharides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The method for extracting and purifying Codonopsis Codonopsis polysaccharides provided by the present invention can be carried out in accordance with the following steps: crush the Codonopsis pilosula from which impurities have been removed, use petroleum ether (60-90° C.) at a mass-volume ratio of 1:6, and keep the temperature at 90° C. Reflux extraction in a water bath for 1 hour, repeat 3 times for degreasing; use 70% (V / V) ethanol for the dry material after degreasing, the mass volume ratio is 1: 6, reflux extraction in a water bath at a constant temperature of 90 ° C for 2 hours, repeat 3 times, filter, discard the filtrate, dry under reduced pressure, evaporate the solvent on the surface of the raw material to obtain the pretreated Codonopsis pilosula raw material; add 6 times the quality of water to the pretreated Codonopsis pilosula raw material, and extract 5 times by reflux in a water bath. 3.5 hours and filter the extract while it is hot; concentrate the extra...

Embodiment 2

[0014] The method for extracting and purifying Codonopsis Codonopsis polysaccharides provided by the present invention can be carried out in accordance with the following steps in sequence: pulverize Codonopsis Codonopsis from which impurities have been removed, use petroleum ether (60-90° C.) at a mass-to-volume ratio of 1:6, and keep the temperature at 70° C. Reflux extraction in a water bath for 3 hours, repeat 6 times for degreasing; use 70% (V / V) ethanol for the dry material after degreasing, 1: 6 by mass volume ratio, reflux extraction in a water bath at a constant temperature of 75°C for 4 hours, repeat 5 times, filter, discard the filtrate, dry under reduced pressure, evaporate the solvent on the surface of the raw material to obtain the pretreated Codonopsis pilosula raw material; 4 hours and filter the extract while it is hot; concentrate the extract under reduced pressure to 1 / 4 of the original volume, add 5 times the volume of absolute ethanol after cooling, let sta...

Embodiment 3

[0016] The method for extracting and purifying Codonopsis Codonopsis polysaccharides provided by the present invention can be carried out in accordance with the following steps: crush Codonopsis Codonopsis from impurities, use petroleum ether (60-90° C.) at a mass-volume ratio of 1:6, and keep the temperature at 80° C. Reflux extraction in a water bath for 2 hours, repeat 5 times for degreasing; use 70% (V / V) ethanol for the dry material after degreasing, 1: 6 by mass volume ratio, reflux extraction in a water bath at a constant temperature of 80 ° C for 3 hours, repeat 4 times, filter, discard the filtrate, dry under reduced pressure, evaporate the solvent on the surface of the raw material to obtain the pretreated Codonopsis pilosula raw material; add 7 times the quality of water to the above pretreated Codonopsis pilosula raw material, and extract 6 times in a water bath with reflux. 2 hours and filter the extract while it is hot; concentrate the extract under reduced pressu...

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PUM

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Abstract

The invention discloses a method for extracting and purifying Codonopsis pilosula polysaccharide. The preparation method comprises steps of: adding water into Codonopsis pilosula raw material pretreated with degreasing and ethanol solution for bath reflux extraction; concentrating an extracting solution; carrying out alcohol precipitation to obtain a Codonopsis pilosula polysaccharide crude extract; and decolorizing and purifying with macroporous adsorption resin to obtain the Codonopsis pilosula polysaccharide extract. The method of the invention can remove most pigment and other impurities in the Codonopsis pilosula polysaccharide and improve the purity of the Codonopsis pilosula polysaccharide, on the premise of no damage on the structure and activity of the Codonopsis pilosula polysaccharide.

Description

technical field [0001] The invention relates to a method for extracting and purifying Codonopsis polysaccharides by using Codonopsis pilosula as raw material. Background technique [0002] Codonopsis polysaccharide is a water-soluble polysaccharide extracted from Codonopsis pilosula. Studies have shown that Codonopsis polysaccharide can accelerate the clearance of serum urea nitrogen in the body, and has the functions of regulating immunity, anti-oxidation, hematopoiesis, anti-aging, and anti-fatigue. In view of Codonopsis polysaccharides having a variety of pharmacological and physiological functions, it can be widely used in pharmaceutical and food industries, so the research on its extraction and purification methods is particularly important. Codonopsis polysaccharide extracts sold in the market often have a darker color due to excessive pigment impurities. Although some Codonopsis polysaccharide extracts are lighter in color, they are often decolorized by oxidation, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 谭智丽杨大玮马麟
Owner 谭智丽
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