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Method for determining acid detergent fiber content in pasture

A technology of acid washing fiber and determination method, which is applied in the direction of weighing by removing certain components, can solve the problems of unsuitable production site measurement, difficulty in ensuring timeliness, etc., and achieve simplification of sample types, improvement of timeliness, and easy mastery Effect

Active Publication Date: 2014-12-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Both of these methods are laboratory methods, which must rely on laboratory conditions to carry out testing work. The requirements for experimental conditions are relatively strict, and it is not suitable for testing at the production site. Therefore, it must go through a long process of sample delivery, analysis, and feedback to obtain accurate data
When the production practice needs to know the feed quality in real time, it is often difficult to ensure the timeliness

Method used

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  • Method for determining acid detergent fiber content in pasture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The mensuration of acid detergent fiber content in embodiment 1, alfalfa

[0042] (1) Alfalfa is air-dried, and then ground to a particle size of 18 meshes with a food grinder.

[0043] (2) Put 1.03 g of the above-mentioned alfalfa and 20 mL of acid washing solution into a preservation bottle (capacity 225 mL), seal it with plastic wrap, and place it in a microwave oven for degassing until it boils until the bottle mouth stops heating. Then add 20mL of acid washing solution in four times, repeat the above degassing process, and add 100mL of acid washing solution in total; during the above degassing process, the power of the microwave oven is 500W, and the degassing time is 1min.

[0044] (3) Add 100mL of acid washing solution to the above-mentioned storage bottle, seal it with a plastic film in a pressure cooker, cap and tighten it, and add it to the neck of the bottle. The pressure cooker is covered and kept pressurized for 30 minutes, and the pressure is 150kpa. of t...

Embodiment 2

[0049] The mensuration of acid detergent fiber content in embodiment 2, Leymus chinensis

[0050] (1) Leymus chinensis is air-dried, and then ground to a particle size of 40 mesh with a food grinder.

[0051] (2) Put 1.13 g of Leymus chinensis above and 20 mL of acid washing solution into a preservation bottle (volume 225 mL), seal it with plastic wrap and place it in a microwave oven for degassing until it boils until the bottle mouth stops heating. Then add 20mL of acid washing solution in four times, repeat the above degassing process, and add a total of 100mL of acid washing solution; during the above degassing process, the power of the microwave oven is 500W, and the degassing time is 2min.

[0052] (3) Add 100mL of acid washing liquid to the above-mentioned preservation bottle, seal it with a plastic film in a pressure cooker, tighten the cap and tighten it, add it to the neck of the bottle, keep the pressure cooker capped for 30 minutes, and the pressure is 170kpa to ob...

Embodiment 3

[0057] The mensuration of acid detergent fiber content in embodiment 3, white clover

[0058] (1) The white clover is air-dried, and then ground to a particle size of 25 meshes with a food grinder.

[0059] (2) Put 0.98g of the above-mentioned white clover and 20mL of acid washing solution into a preservation bottle (volume 225mL), seal it with plastic wrap and place it in a microwave oven for degassing until it boils until the bottle mouth stops heating. Then add 20mL of acid washing solution in four times, repeat the above degassing process, and add 100mL of acid washing solution in total; during the above degassing process, the power of the microwave oven is 500W, and the degassing time is 3min.

[0060] (3) Add 100mL of acid washing solution to the above-mentioned preservation bottle, seal it with a plastic film with a pressure cooker, cover and tighten it, add it to the neck of the bottle, cover the pressure cooker and keep pressurizing for 30 minutes, the pressure is 160...

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Abstract

The present invention provides a method for determining acid detergent fiber content in pasture. The method includes the following steps: (1) mixing and placing the pasture with a weight of A and an acid washing liquid in a preservation bottle, and placing the preservation of the bottle in a microwave oven for degassing; (2) sealing the preservation bottle and pressurizing the bottle in a pressure cooker to obtain a mixed liquid; (3) filtering the mixed liquid obtained in the step (2) with a filter with a weight of B to obtain a filter cake namely the acid detergent fiber; and (4) drying the filter and the filter cake to a constant weight of C and then calculating according to a formula (I) to obtain the acid detergent fiber (ADF) content in the pasture (%). Through depth analysis of ADF measurement principle, the present invention develops a simple method for determining ADF content in pasture.

Description

technical field [0001] The invention relates to a method for determining the content of acid detergent fiber in pasture, belonging to the technical field of pasture detection. Background technique [0002] Acid Detergent Fiber (ADF) is one of the important indicators in Van Soest's detergent fiber analysis program. Forage feed is heated and dissolved in acidic detergent solution. The dissolved part is the cell content and hemicellulose in the cell wall. , The insoluble residue is acid detergent fiber, mainly cellulose, lignin, pectin and some silicates. Acid detergent fiber is an important index parameter to evaluate feed quality and guide the use of forage feed. Correctly grasping this parameter is of great significance for guiding forage production, product trade, animal diet matching, and animal health. [0003] Acid detergent fiber index is an index often used in production. At present, it mainly relies on analysis and testing laboratories to complete the determination....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N5/04
Inventor 玉柱白春生张英俊娜日苏许庆方
Owner CHINA AGRI UNIV