A kind of gas chromatography fingerprint detection method of traditional Chinese medicine composition

A detection method and fingerprint technology, applied in the field of gas chromatography fingerprint detection, can solve the problem of single component

Active Publication Date: 2016-02-24
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Simultaneously, in the prior art, though also have the method that this medicine is carried out gas chromatography detection, the component that detects is all relatively single, generally one or two components, as cinnamon aldehyde or paeonol, only rely on these two These components cannot fully reflect the actual quality of the medicinal composition of Guizhi Fuling

Method used

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  • A kind of gas chromatography fingerprint detection method of traditional Chinese medicine composition
  • A kind of gas chromatography fingerprint detection method of traditional Chinese medicine composition
  • A kind of gas chromatography fingerprint detection method of traditional Chinese medicine composition

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Experimental program
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Effect test

Embodiment 1

[0098] (1) Chromatographic conditions and system adaptability test: capillary column is selected; the temperature of the injection port is 220°C; the detector is a hydrogen flame ionization detector, and the detector temperature is 230°C; Raise the temperature at 15°C / min to 150°C and keep it for 5 minutes, then raise it to 240°C at a speed of 25°C / min and keep it for 13 minutes; the split ratio is 20:1; N 2 The flow rate is 0.5mL / min; the number of theoretical plates shall not be less than 3000 based on the calculation of the reference substance paeonol.

[0099] (2) Preparation method of the reference substance solution: take an appropriate amount of paeonol reference substance, weigh it accurately, add ether to prepare a solution containing 0.2 mg per 1 mL, and use paeonol as the reference substance.

[0100] (3) The preparation method of the test solution: take 2.0 g of Guizhi Fuling Pills, add 40 mL of water and 20 mL of ether, reflux in a water bath at 65 ° C for 1.0 h, ...

Embodiment 2

[0105] (1) Chromatographic conditions and system adaptability test: capillary column was selected; the temperature of the injection port was 225°C; the detector was a hydrogen flame ionization detector, and the detector temperature was 235°C; the column temperature was 75°C, and kept for 2.5min. Raise the temperature at 18°C / min to 155°C and keep it for 5.5 minutes, then raise the temperature at 28°C / min to 245°C and keep it for 13.5 minutes; the split ratio is 23:1; N 2 When the flow rate is 0.8mL / min, the number of theoretical plates shall not be less than 3000 based on the calculation of the reference substance paeonol.

[0106] (2) Preparation method of reference substance solution: take an appropriate amount of paeonol reference substance, weigh it accurately, add ether to prepare a solution containing 0.6 mg per 1 mL, and use paeonol as the reference substance.

[0107] (3) The preparation method of the test solution: take about 3.0 g of the cinnamon twig and poria cocos...

Embodiment 3

[0112] (1) Chromatographic conditions and system adaptability test: capillary column is selected; the temperature of the injection port is 230°C; the detector is a hydrogen flame ionization detector, and the detector temperature is 240°C; Raise the temperature at 20°C / min to 160°C and keep it for 6 minutes, then raise it to 250°C at a speed of 30°C / min and keep it for 14 minutes; the split ratio is 25:1; N 2 The flow rate is 1.0mL / min; the number of theoretical plates shall not be less than 3000 based on the calculation of the reference substance paeonol.

[0113] (2) Preparation method of reference substance solution: take an appropriate amount of paeonol reference substance, weigh it accurately, add ether to prepare a solution containing 1mg per 1mL, and use paeonol as the reference substance.

[0114] (3) The preparation method of the test solution: take 3.5g of Guizhi Fuling Capsules, add 50mL of water and 30mL of ether, reflux in a water bath at 75°C for 1.0h, separate th...

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Abstract

The invention belongs to the field of analysis of traditional Chinese medicines and specifically relates to a method for detecting the pharmaceutical composition of cassia twig and Poria cocos by using gas chromatography and fingerprint chromatography. Chromatographic conditions and system adaptability test are as follows: a capillary column is used; injection port temperature is 220 to 240 DEG C; a hydrogen flame ionization detector is used and has a temperature of 230 to 250 DEG C; column temperature is 70 to 90 DEG C which is maintained for 2 to 4 min, and then the capillary column is heated at a speed of 15 to 25 DEG C / min to a temperature of 150 to 170 DEG C and maintained at the temperature for 5 to 7 min and then is heated at a speed of 25 to 35 DEG C / min to a temperature of 240 to 260 DEG C and maintained at the temperature for 13 to 15 min; a split ratio is 20-30: 1; N2 flow velocity is 0.5 to 2.0 mL / min; and the number of theoretical plates, in terms of the reference substance of paeonol, should not be lower than 3000. The method provided in the invention can more comprehensively detect the quality of the composition, is more beneficial for controlling of the quality of the composition and has the advantages of high precision and good stability and repeatability.

Description

Technical field: [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a gas chromatography fingerprint detection method of a Chinese medicine composition, in particular to a gas-phase gas-phase analysis of a Chinese medicine composition made of cassia twigs, poria cocos, white peony root, moutan cortex and peach kernels as raw materials. Chromatographic fingerprint detection method. Background technique: [0002] The medicinal composition of Guizhi Fuling is made of Chinese medicine Guizhi, Poria, Paeoniae Alba, Cortex Moutan, and peach kernel. diseases such as fibroids. The capsule preparation of the traditional Chinese medicine composition has been approved for marketing by the State Food and Drug Administration of China. The generic name of the drug is Guizhi Fuling Capsule, and the drug approval number is Z10950005. [0003] The applicant of the present invention has studied the preparation process of the cinnamon twig and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 萧伟王振中毕宇安李家春章晨峰陈凤龙孙兰李红娟于桂芳张帅徐海娟
Owner JIANGSU KANION PHARMA CO LTD
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