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Two CAPs markers tightly linked with muskmelon anti-powdery mildew gene Pm-AN and its application method

A technology for resistance to powdery mildew and melon, applied in the direction of DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as difficulty in distinguishing homozygous and heterozygous genotypes, and difficulty in realizing aggregation of different powdery mildew resistance genes, so as to avoid chain burden , the effect of improving the selection efficiency

Inactive Publication Date: 2012-10-24
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Traditional breeding methods are difficult to distinguish between homozygous and heterozygous genotypes, it is difficult to aggregate different powdery mildew resistance genes, and it is impossible to select individuals recombined near disease resistance genes

Method used

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  • Two CAPs markers tightly linked with muskmelon anti-powdery mildew gene Pm-AN and its application method
  • Two CAPs markers tightly linked with muskmelon anti-powdery mildew gene Pm-AN and its application method
  • Two CAPs markers tightly linked with muskmelon anti-powdery mildew gene Pm-AN and its application method

Examples

Experimental program
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Effect test

Embodiment Construction

[0023] Example Selection of homozygous disease-resistant lines by molecular markers linked to the powdery mildew resistance gene Pm-AN of melon

[0024] 1. Experimental materials

[0025] The parental materials for melon mapping, Annong No. 2 and Hami melon K413, were provided by Xinjiang National Melon Engineering Technology Research Center. Annong No. 2 was introduced from Japan by Professor Lin Depei in 1986. It is high-quality and high-yield. It is resistant to various diseases such as powdery mildew, vine cutting, viral disease, downy mildew and wilt. The fruit is round, with white skin and white flesh. , small fruit type muskmelon (15cm×12cm). K413 is a typical Hami melon type. The melon plant has poor disease resistance, oval fruit, yellow skin and yellow flesh, crisp taste, and a large melon shape (30cm×16cm).

[0026] 2. Experimental method

[0027] 2.1 Extraction of Xinjiang muskmelon genomic DNA

[0028] (1) Take about 50mg of fresh melon leaves in an EP tube, a...

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Abstract

The invention relates to two codominance CAPs molecular markers which are positioned at two sides of muskmelon anti-powdery mildew gene Pm-AN and tightly linked with Pm-AN, and its application method, which belongs to the biology technical field. The markers are used for muskmelon anti-powdery mildew molecular markers for auxiliary breeding, and breeding purpose and breeding efficiency of the muskmelon anti-powdery mildew breeding can be substantially enhanced. The application of the markers is not influenced by disease factors in fields, can effectively select pure disease resistance gene type and heterozygosis disease resistance gene type individuals, and the recombinant genotype individuals are generated at two sides of the anti-powdery mildew gene. The markers can be used for disease resistance molecule polymerization breeding without transgene security problem.

Description

technical field [0001] The invention relates to two co-dominant CAPs molecular markers located on both sides of the melon powdery mildew resistance gene Pm-AN and closely linked with the Pm-AN and a use method thereof, belonging to the field of biotechnology. Background technique [0002] A broad molecular marker refers to an heritable and detectable DNA sequence or protein. Molecular markers in a narrow sense only refer to DNA markers, which are a direct reflection of genetic polymorphism at the DNA level, and the concept of molecular markers is generally defined within this category. The heritable variation produced by organisms in the long-term evolution process is all due to the variation of DNA base sequence. There are extremely rich DNA base sequence variations in the same species, which has laid a solid foundation for the development and utilization of molecular markers. [0003] DNA molecular marker refers to the specific DNA that reflects a certain variation chara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 王贤磊李冠高兴旺马东建宁雪飞
Owner XINJIANG UNIVERSITY
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