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Method for preparing water-soluble plant melanin

A manufacturing method and melanin technology, applied in the field of plant melanin manufacturing, can solve the problems of impossible water solubility, destroying the three-dimensional structure of melanin, unable to restore water solubility of melanin, etc., and achieving the effect of excellent water solubility.

Active Publication Date: 2012-10-24
ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The natural plant melanin produced by the general method is insoluble in water, the fundamental reason is that the melanin in the plant tissue is a complex three-dimensional polymer, which is mixed with proteins, lipids, other pigments or the above If they are firmly combined, it is impossible to have water solubility, and the usual lye extraction method only destroys a part of the three-dimensional structure of melanin, and cannot restore melanin to water solubility

Method used

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  • Method for preparing water-soluble plant melanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Get 1kg of fresh black rice leaves, mash it with a tissue masher, and prevent the local temperature from exceeding 60°C during the mash process. Transfer to a reaction kettle, add 2L of 60% (v / v) ethanol aqueous solution, extract at a temperature of about 50°C for 2.5 hours, filter off the residue to obtain a filtrate, remove ethanol under reduced pressure until there is no alcohol smell, and the obtained concentrated The liquid was supplemented with deionized water to the initial volume of the filtrate, and passed through the ultrafiltration membrane of AMICON-polysulfone P100 to obtain an aqueous solution of the melanin precursor, and the total phenolic mass in the melanin precursor solution was measured as 24.5g according to GB8313-87.

[0036] 2.45g (i.e. 10wt.%) polyphenol oxidase (the specific activity of this polyphenol oxidase is more than 10000 units / g) is dropped into the melanin precursor solution, under the condition of 37 ℃, insulation reaction 5hr, and with...

Embodiment 2

[0040] Get 1kg of fresh black rice leaves, mash it with a tissue masher, and prevent the local temperature from exceeding 60°C during the mash process. Transfer to a reaction kettle, add 1L of 80% (v / v) acetone aqueous solution, extract at a temperature of about 25°C for 2.5 hours, filter off the residue to obtain a filtrate, remove acetone under reduced pressure until there is no acetone smell, and concentrate the obtained The liquid was supplemented with deionized water to the initial volume of the filtrate, and passed through the ultrafiltration membrane of AMICON-polysulfone P100 to obtain an aqueous solution of the melanin precursor, and the total phenolic mass in the melanin precursor solution was measured as 22.1g according to GB8313-87.

[0041] 4.42g (i.e. 20wt.%) polyphenol oxidase (the specific activity of this polyphenol oxidase is more than 10000 units / g) is dropped into the melanin precursor solution, under the condition of 35 ℃, insulation reaction 3hr, and with ...

Embodiment 3

[0045] Get 1kg of fresh black rice leaves, mash it with a tissue masher, and prevent the local temperature from exceeding 60°C during the mash process. Transfer to a reaction kettle, add 3L of 50% (v / v) acetone aqueous solution, extract at a temperature of about 50°C for 2.5hr, filter off the residue to obtain a filtrate, remove acetone under reduced pressure until there is no acetone smell, and concentrate the obtained The liquid was supplemented with deionized water to the initial volume of the filtrate, and passed through an ultrafiltration membrane of AMICON-polysulfone P100 to obtain an aqueous solution of the melanin precursor, and the mass of total phenols in the melanin precursor solution was measured as 25.0 g according to GB8313-87.

[0046] 25.0g (i.e. 100wt.%) polyphenol oxidase (the specific activity of this polyphenol oxidase is more than 10000 units / g) is dropped into the melanin precursor solution, under the condition of 42 ℃, insulation reaction 2hr, and with 5...

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Abstract

The invention discloses a method for preparing a water-soluble plant melanin. The method comprises the following steps of preparing a melanin precursor from blueberry leaves as raw materials and carrying out enzyme catalytic oxidation, macroporous resin purification and spray drying to obtain the water-soluble plant melanin. The water-soluble plant melanin obtained by the method has good water-solubility and solves the problem that a natural melanin cannot be dissolved in water.

Description

technical field [0001] The present invention relates to melanin and its production method, specifically refers to the production method of obtaining a highly water-soluble plant melanin by using the leaves of black rice plant as raw material through biological enzymatic reaction. Background technique [0002] Melanin is a natural barrier to protect tissues by absorbing ultraviolet rays and ionizing radiation, which widely exists in the biological world. The research on natural melanin has been more than half a century. Although the research is not completely thorough, the basic synthesis route and physical and chemical properties have been relatively clear: melanin from animal and microbial sources is mainly tyrosine and other tyrosine. Catalyzed oxidation by acidase, finally synthesize melanin through dopa, dopaquinone, indolequinone and other pathways; phytomelanin is catechol and other polyphenols containing ortho-phenolic hydroxyls oxidized to quinones and polymerized by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00
Inventor 魏国华邵斌许新德吕良伟虞雷明刘云凤
Owner ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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