Beta2-microglobulin detection kit and preparing method thereof

A detection kit and microglobulin technology, applied in the field of biomedicine, can solve the problems of time-consuming, waste of resources, cumbersome operation, etc.

Inactive Publication Date: 2012-10-24
乐普(北京)诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the disadvantages of cumbersome operation, long time consump

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1:

[0033] An example of the kit of the present invention is a dual reagent, in which:

[0034] Reagent R1:

[0035] Tris buffer 200mmol / L

[0036] Polyethylene glycol 8 mmol / L

[0037] Sodium azide 5 mmol / L

[0038] Sodium ethylenediaminetetraacetate 5mmol / L

[0039] Bovine serum albumin 5mmol / L;

[0040] Reagent R2:

[0041] Tris buffer 200mmol / L

[0042] Latex particles with β2-microglobulin monoclonal antibody 5% (v / v)

[0043] Tween-20 5mmol / L

[0044] Sodium azide 3 mmol / L

[0045] Sodium ethylenediaminetetraacetate 5mmol / L

[0046] Bovine serum albumin 5mmol / L.

Example Embodiment

[0047] Example 2: How to use the kit

[0048] 1. Reagent preparation, the reagents are liquid double reagents, ready to use after opening the bottle, of which:

[0049] Reagent R1:

[0050] Tris buffer 200mmol / L

[0051] Polyethylene glycol 8 mmol / L

[0052] Sodium azide 5 mmol / L

[0053] Sodium ethylenediaminetetraacetate 5mmol / L

[0054] Bovine serum albumin 5mmol / L;

[0055] Reagent R2:

[0056] Tris buffer 200mmol / L

[0057] Latex particles with β2-microglobulin monoclonal antibody 5% (v / v)

[0058] Tween-20 5mmol / L

[0059] Sodium azide 3 mmol / L

[0060] Sodium ethylenediaminetetraacetate 5mmol / L

[0061] Bovine serum albumin 5mmol / L.

[0062] 2. Automatic biochemical analyzer parameter setting

[0063] (a) Detection temperature: 37℃

[0064] (b) Detection wavelength: the main wavelength is 550nm; the secondary wavelength is 700nm;

[0065] (c) Reaction time: 8 minutes, in which the incubation time is 5 minutes, the reaction time is 0.5 minutes, and the detection time is 2.5 minutes.

[0066] 3....

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Abstract

A beta2-microglobulin detection kit contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared by buffer solution, polyethylene glycol, sodium azide, edetic acid and bovine serum albumin, and the reagent R2 is prepared by buffer solution, emulsion particles combined with beta2-microglobulin monoclonal antibodies, polysorbate-20, sodium azide, edetic acid and bovine serum albumin. Samples and the reagents are mixed according to a certain volume ratio and led to have a series of reactions, then reactants are placed under a semi-automatic/full-automatic biochemical analyzer, absorbance change speed of the reactants at the position with dominant wavelength as 550nm is detected, and accordingly concentration of beta2-microglobulin is measured and calculated. The kit has the advantages of being accurate, stable and convenient.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a detection kit for detecting human β2-microglobulin. Background technique [0002] β2-microglobulin is a single-chain polypeptide low-molecular protein composed of 100 amino acid residues. Electrophoresis is in the β2 area, so it is called β2-microglobulin. Originated from human mesenchyme, normal cells of epithelial cells and hematopoietic system as well as malignant tumor cells can synthesize β2-microglobulin. [0003] β2-microglobulin has a relatively small molecular weight, can be freely filtered through the glomerulus, and is only excreted by the kidneys. The production rate in the body is constant, and it is not affected by age, sex, and the amount of muscle tissue in the body. Therefore, serum β2-microglobulin is Early indicator of kidney damage. [0004] The increase of serum β2-microglobulin can reflect the impairment of glomerular filtration function or whether ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 张雷
Owner 乐普(北京)诊断技术股份有限公司
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