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Method for gathering and purifying general flavone from Helianthus tuberosus L. leaves by adopting macroporous resin

A technology of macroporous resin and total flavonoids, applied in the directions of botanical equipment and methods, pharmaceutical formulations, cosmetic formulations, etc.

Active Publication Date: 2012-11-07
北京碧青园生态环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies on flavonoids in Jerusalem artichoke leaves are rarely reported

Method used

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  • Method for gathering and purifying general flavone from Helianthus tuberosus L. leaves by adopting macroporous resin
  • Method for gathering and purifying general flavone from Helianthus tuberosus L. leaves by adopting macroporous resin

Examples

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Embodiment 1

[0015] The dried Jerusalem artichoke leaves are pulverized with a pulverizer and passed through a sieve with an aperture of 4 mm to obtain a powder with a particle size of less than 4 mm. Add 50% vt of ethanol 8 times the weight of the raw material to reflux the pulverized raw material for 3 times, each extraction After 2 hours, filter, combine the filtrate, concentrate the filtered extract under reduced pressure to recover ethanol, and detect the concentrated concentration to the macroporous resin loading concentration, that is, the mass concentration of total flavonoids 1.5mg / mL. The extract concentrate is transparent brown Yellow liquid, adjust the pH of the concentrated solution to 5-6, add the solution to the D101 macroporous resin column for saturated adsorption, the sample flow rate is 1.0mL / min, the maximum sample volume is 1.5 times the column volume, first use 5 times the column volume The impurities are removed by elution with 5 times the column volume of 70% ethanol....

Embodiment 2

[0023] The dried Jerusalem artichoke leaves are pulverized with a pulverizer and passed through a sieve with an aperture of 4mm to obtain a powder with a particle size of less than 4mm. Add 70% ethanol 10 times the weight of the raw material to reflux the pulverized raw material for 3 times, each extraction 2 times After hours, filter, combine the filtrate, concentrate the filtered extract under reduced pressure to recover ethanol, check the concentrated concentration to the macroporous resin loading concentration, that is, the mass concentration of total flavonoids 2.4mg / mL, the extract concentrate is transparent brownish yellow Liquid, adjust the pH of the concentrated solution to 5-6, add the solution to the D101 macroporous resin column for saturated adsorption, the sample flow rate is 1.0mL / min, the maximum sample volume is 1.5 times the column volume, first use 5 times the column volume Water is eluted to remove impurities, and 5 times the column volume of 70% ethanol is u...

Embodiment 3

[0025] The dried Jerusalem artichoke leaves are pulverized with a pulverizer and passed through a sieve with an aperture of 4 mm to obtain a powder with a particle size of less than 4 mm. Add 90% ethanol of 12 times the weight of the raw material to reflux the pulverized raw material for 3 times, each extraction 2 times After hours, filter, combine the filtrate, concentrate the filtered extract under reduced pressure to recover ethanol, check the concentrated concentration to the macroporous resin loading concentration, that is, the mass concentration of total flavonoids 3.6mg / mL, the extract concentrate is transparent brownish yellow Liquid, adjust the pH of the concentrated solution to 5-6, add the solution to the D101 macroporous resin column for saturated adsorption, the sample flow rate is 1.0mL / min, the maximum sample volume is 1.5 times the column volume, first use 5 times the column volume Water eluted to remove impurities, and then 5 times the column volume of 70% ethan...

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Abstract

A method for gathering and purifying general flavone from Helianthus tuberosus L. leaves by adopting macroporous resin comprises the following technological steps: Helianthus tuberosus L. leaves are cleaned and then dried at low temperature; the dried leaves are crushed by a grinder; the crushed leaves are screened in a sieve with the aperture of 4 mm; 50-90 percent of alcohol, which is 8-12 times in weight of the raw material, is added for reflux extraction of 2-3 times (each time of 1-2 hours); the extract is filtered, merged and then concentrated under reduced pressure to recover the alcohol so as to reach the loading concentration of the macroporous resin after concentration, that is, the concentration of general flavone is 1.5-3.6 mg / mL; the solution is added into the macroporous resin for saturaion adsorption, and the maximum loading quantity is 1.5 times of column volume; eluting and removing impurities by water of 5 times of column volume at first; then 70 percent of alcohol of 5 times of column volume is taken as an eluant; and the eluant is subject to recovered solvent and concentration treatment and is ultimately dried to obtain the general flavone. The content of the general flavone extractive obtained by adopting the method can reach more than 80 percent after the general flavone extractive is purified. The invention is provided for the first time to gather and purify general flavone from Helianthus tuberosus L. leaves by adopting macroporous resin, and the method provided by the invention is simple in process, low in cost, easy in operation and high in efficiency, can obtain an extractive in which the content of general flavone is high, and has very good development and utilization values.

Description

Technical field [0001] The invention relates to a method for preparing plant effective ingredients, in particular to a method for purifying total flavonoids from Jerusalem artichoke leaves. Background technique [0002] Jerusalem artichoke (Helianthus tuberosus L.), commonly known as Jerusalem artichoke, is a perennial herb of the genus Helianthus in the Asteraceae family. Native to North America, it was introduced to Europe in the 17th century and then to China. It is widely distributed, adaptable, easy to grow, high yield, and low cost. Its tubers can be used to ferment to produce ethanol, and can also be hydrolyzed to produce high-fructose syrup. Its leaf extract has good bactericidal activity and can be used to develop plant-derived antibacterial agents. [0003] Flavonoids are ubiquitous secondary metabolites in plants, and have a variety of beneficial physiological and pharmacological effects. Studies have found that flavonoids have important medicinal values ​​such as pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/12A01P1/00A61K36/28A61K8/97A61P31/04
Inventor 刘玲隆小华郑晓涛刘兆普
Owner 北京碧青园生态环境科技有限公司