Fusion protein for screening and evaluating anti-enterovirus 71 medicine and application of fusion protein

A fusion protein and enterovirus technology, applied in the biological field, can solve the problems of difficulty in evaluating the anti-EV71 effect of drugs, complicated operation, and lack of evaluation system
CN102766607AInactive Publication Date: 2012-11-07HARBIN MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
HARBIN MEDICAL UNIVERSITY
Publication Date
2012-11-07
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses fusion protein for screening and evaluating an anti-enterovirus 71 (EV 71) medicine and application of the fusion protein. According to the fusion protein, firefly luciferase (Fluc) is used as a reporter gene, two micromolecule polypeptides which can be bonded tightly pepA and pepB are bonded with the N end and the C end of the firefly luciferase respectively, the middles of the firefly luciferase are connected by an EV713C protease effect substrate, and a fusion gene is inserted into an eukaryotic expression vector and is expressed to generate the fusion protein. Simultaneously, enhanced green fluorescent protein (EGFP) which is inserted reversely can indicate the transfection efficiency of the EGFP in cells which are transfected in vitro by observing the condition of green fluorescence. By utilizing an indication vector provided by the fusion protein, the reproduction condition of EV 71 can be indicated simply, quickly, flexibly and quantitatively, and the indication vector also can be applied to the screening and evaluation of the anti-EV 71 medicine, has high actual application value and has a broad application prospect in the field of medical science.
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Description

technical field

[0001] The invention relates to a fusion protein for screening and evaluating drugs and its application, in particular to a fusion protein for screening and evaluating anti-EV71 drugs based on a chemiluminescence method and its application. Belongs to the field of biotechnology. Background technique

[0002] Enterovirus 71 (enterovirus 71, EV71) is an important neuroinfectious enterovirus, and currently there is no effective treatment and vaccine. There are reports of outbreaks of infectious diseases associated with EV71 virus infection in the Asia-Pacific region and around the world (AbuBakar, S., et al.. Enterovirus71 outbreak, Brunei. Emerg. Infect. Dis. 2009, 15:79-82.) . EV71 infection mainly causes a rash in children known as hand-foot-mouth disease (HFMD). However, acute infection with EV71 can also cause severe neurological disease and high mortality. In particular, EV71 infection in children under five years of age has been associated with neurol...

Claims

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