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Culture medium cold-filling preparation process

A preparation process and culture medium technology, applied in the field of culture medium preparation, can solve the problems of loss of nutrients, increase production costs, increase excessive degradation of nutrients, and achieve the effects of improving production efficiency, reducing production costs, and avoiding excessive degradation.

Inactive Publication Date: 2014-04-02
TAIZHOU QIANYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the whole preparation process, constant solution heating equipment is needed, and the culture medium needs a high-temperature heating link before moist heat sterilization. On the one hand, the investment in equipment is increased, space is occupied, and production costs are increased. On the other hand, the high-temperature heating link increases Chance of excessive degradation of nutrients, resulting in loss of nutrients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Measure various mother liquors required for Dendrobium officinale culture medium, add 30-50 liters of pure water into the filling machine and adjust the stirring speed to 210 rpm, add macroelements, trace elements, organic Elements, sugar, agar and other ingredients, stir while adding to fully mix and dissolve the ingredients; add pure water to dissolve to 80 liters, continue stirring for 12 minutes; measure the pH value of the solution and adjust the pH value to 6.0; put the filling machine cover Put the lid on, adjust the air pressure to 0.01mpa, adjust the filling volume to 100-120ml / bottle, and start filling; after the filling is completed, cover the bottle cap, wrap the sealing film and put it in a sterilizer to start sterilization, at a temperature of 121°C Sterilize for 20 minutes; put the sterilized medium into the medium storage room for 3 days before use.

Embodiment 2

[0022] Measure the various ingredients needed for the Phalaenopsis culture medium, mix and dissolve the measured ingredients, add 30-50 liters of pure water to the filling machine and adjust the stirring speed to 170 rpm, and then fill the Add a large number of elements, trace elements, organic elements, sugar, agar and other ingredients into the machine, stir while adding to make the ingredients fully mixed and dissolved; add pure water to dissolve to 80 liters, continue to stir for 15 minutes; measure the pH value of the solution and Adjust the pH value to 5.5; cover the filling machine, adjust the air pressure to 0.015mpa, adjust the filling volume to 100-120ml / bottle, and start filling; after the filling is completed, cover the bottle cap, wrap the sealing film and put it in The sterilizer starts to sterilize, and sterilizes at a temperature of 115°C for 22 minutes; the sterilized medium is put into the medium storage room for 3 days before use.

Embodiment 3

[0024] Measure the various mother liquors required for the clematis culture medium, add 30-50 liters of pure water into the filling machine and adjust the stirring speed to 270 rpm, then add macroelements, trace elements, Add organic elements, sugar, agar and other ingredients, stir while adding to fully mix and dissolve the ingredients; add pure water to 80 liters, continue stirring for 8 minutes; measure the pH value of the solution and adjust the pH value to 6.5; put the filling machine Close the lid, adjust the air pressure to 0.02mpa, adjust the filling volume to 100-120ml / bottle, and start filling; after the filling is completed, close the bottle cap, wrap the sealing film and put it in a sterilizer to start sterilization, at 125°C Sterilize at high temperature for 18 minutes; put the sterilized medium into the medium storage room for 3 days before use.

[0025] The cold filling preparation process of the above medium does not require heating and dissolving. Compared wit...

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Abstract

The invention discloses a culture medium cold-filling preparation process. The preparation process includes following steps: weighing solid or liquid components of reagents needed for culture medium, dissolving and mixing the weighed culture medium components, adding pure water 30%-50% of the volume into a filling machine, adding the dissolved culture medium components while stirring, subjecting the culture medium mixture to constant dissolving, adjusting PH (potential of hydrogen) value, adjusting the rotation speed of the filling machine to be 150-270r / min for stirring, adjusting the pneumatic pressure of the filling machine to be 0.01-0.02MPa, filling at the normal temperature, and covering and sealing the filled culture medium for steam moist heat sterilization. Preparation of the culture medium can be completed only by weighing instrument equipment and packaging sterilization equipment without constant-dissolving and heating equipment, so that preparation time of the culture medium is shortened, production efficiency is improved, production cost is lowered, energy is saved and emission is reduced, excessive degradation of nutritional components is avoided, and various nutritional components of the culture medium are protected from being damaged.

Description

technical field [0001] The invention relates to a preparation process of a medium, in particular to a preparation process of a medium cold filling. Background technique [0002] At present, there are many kinds of medium, which are widely used in various technical fields such as biotechnology. The traditional medium preparation process usually adopts the following steps: Weighing the components of the medium, fully dissolving and mixing the components of the medium, and then heating to Stir while heating at a certain temperature to fully dissolve and mix the ingredients. After cooling, adjust the pH value to the specified value range, and then fill it. Cover the filled culture medium with a sealing film and then carry out steam damp heat sterilization. During the whole preparation process, constant solution heating equipment is needed, and the culture medium needs a high-temperature heating link before moist heat sterilization. On the one hand, the investment in equipment is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B67C7/00
Inventor 胡金全李宏林王慧蕾王文斌左俊
Owner TAIZHOU QIANYUAN BIO TECH
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