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Isaria cretacea and application thereof
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A technology of I. cephalospora and bacterial strains, applied in the field of I. cephalospora
Inactive Publication Date: 2014-05-07
蔡春 +1
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The strain was preserved in the General Microorganism Center of China Committee for Microorganism Culture Collection (CGMCC for short) on June 07, 2012.
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Embodiment 1
[0058] Embodiment 1, the preparation of Isaria Cretacea (Isaria Cretacea) A-01CGMCC No.6204 strain
[0059] Inoculate Isaria Cretacea (Isaria Cretacea) A-01CGMCC No.6204 on the slant of PDA medium, and culture it under dark conditions at 18°C for 14 days to obtain Isaria Cretacea A-01CGMCC No. .6204 strains.
[0061] 1. Preparation of strain solution: Isaria Cretacea (Isaria Cretacea) A-01CGMCC No.6204 strain was inoculated in PD medium, and cultured by shaking at 22°C, rotating speed 150rpm, and dark for 72 hours to obtain the strain liquid.
[0063] Crumble the sterilized indica rice medium through the strain bag, inoculate each bag with 15ml of the strain solution under aseptic conditions, continue the aseptic operation, seal the mouth with a cotton plug, and knead evenly through the strain bag Finally, put them into the culture room, and place them as flat as possible on the culture rack without touching the tampon. Under dark conditions, culture at 18°C for 21 days. After 21 days, remove all cultures in the seed bag (such as figure 1, including culture medium, conidia and mycelia) were dried at 80°C for 72 hours, dried, crush...
Embodiment 3
[0064] Example 3, Isaria Cretacea (Isaria Cretacea) A-01CGMCC No.6204 has a protective effect on the bone marrow and small intestine of X-ray irradiated mice
[0065] In this example, the spleen nodule formation method was used to observe the survival of bone marrow stem cells in BALB / c mice after taking the Isaria Cretacea A-01CGMCC No.6204 myceliumpowder of Example 2 and being irradiated with different doses of X-rays Situation, be contrast with simply being irradiated and normal mouse; Observation BALB / c and KM mouse take embodiment 2 Isaria Cretacea (Isaria Cretacea) A-01CGMCC No.6204 bacterium with regeneration method of small intestinal crypt cell The regeneration of small intestinal crypt cells after the silk powder was irradiated with different doses of X-rays at different times was compared with that of the simple irradiation group.
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Abstract
The invention discloses isaria cretacea and application thereof. The isaria cretacea strain is isaria cretacea A-01of CGMCC (China General Microbiological Culture Collection) No. 6204 registered at a general microbiology center of a China microbial culture collection management committee. The isaria cretacea A-01 and a bacterium agent taking the same as an active ingredient can be used for preparing a product with at least one function: 1) a protective effect on animal bone marrow and small intestine which are irradiated by X-ray; 2) a protective effect on animal chromosomal damage; 3) enhancing of killing role of rays on tumors; 4) cellimmune regulation effect; 5) humoral immune regulation; 6) treatment of palpitation; 7) treatment of shortness of breath; 8) treatment of lacking in strength; 9) treatment of chest tightness; 10) treatment of anorexia; 11) treatment of constipation; 12) treatment of insomnia; and 13) treatment of dreaminess.
Description
technical field [0001] The invention relates to a strain of Isosporium cephalospora and its application. Background technique [0002] At present, the most effective method for treating tumors in the world is surgery, as well as chemotherapy and radiotherapy after surgery. However, if only surgery is used to treat tumors, the recurrence rate of tumors cured in this way will be quite high in the future. Diet therapy is used as an aid, and the side effects of chemotherapy and radiotherapy on the human body are very high, which brings great pain to the patients. Therefore, in fact, at certain times, the survival time of patients after radiotherapy and chemotherapy is better than that before chemotherapy and radiotherapy. Longer, all modern medicine began to carry out comprehensive treatment plans. What is comprehensive treatment? The so-called comprehensive treatment is to take health food that can relieve the damage and pain to the body after radiotherapy and chemotherapy befo...
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