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Manufacturing method for sugar solution and device for same

A manufacturing method and technology of sugar liquid, which are applied in the directions of sugar production, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problem of high enzyme cost, and achieve the effects of increasing sugar production and recovery.

Inactive Publication Date: 2012-11-21
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a disadvantage of such a method using an enzyme, there is a problem that the cost of the enzyme is high.

Method used

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  • Manufacturing method for sugar solution and device for same
  • Manufacturing method for sugar solution and device for same
  • Manufacturing method for sugar solution and device for same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0133] Hereinafter, the present invention will be described more specifically by way of examples. However, the present invention is not limited to these Examples.

reference example 1

[0134] (Reference Example 1) Preparation of Cellulose Pretreatment

[0135] 1. Preparation of cellulose pretreatment 1 (dilute sulfuric acid treatment)

[0136] Rice straw was used as cellulose. The cellulose was immersed in a 1% aqueous solution of sulfuric acid, and autoclaved at 150° C. for 30 minutes (Nitto Kozoku). After the treatment, solid-liquid separation is carried out to separate into sulfuric acid aqueous solution (hereinafter referred to as dilute sulfuric acid treatment solution) and sulfuric acid treatment cellulose. Next, the sulfuric acid-treated cellulose was stirred and mixed with the dilute sulfuric acid-treated liquid so that the solid content concentration was 10% by weight, and then the pH was adjusted to about 5 with sodium hydroxide. This product was used as cellulose pretreatment 1 in the following examples.

[0137] 2. Preparation of cellulose pretreatment 2 (ammonia treatment)

[0138] Rice straw was used as cellulose. The above-mentioned cellulo...

reference example 2

[0141] (Reference example 2) Measurement of sugar concentration

[0142] The concentrations of glucose and xylose contained in the sugar solution were quantified by comparison with standard products under the HPLC conditions shown below.

[0143] Column: Luna NH 2 (manufactured by Phenomenex)

[0144] Mobile phase: Milli-Q:acetonitrile=25:75 (flow rate 0.6mL / min)

[0145] Reaction solution: no

[0146] Detection method: RI (differential refractive index)

[0147] Temperature: 30°C.

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Abstract

A method for cellulolysis which uses a cellulase from a filamentous fungus as a saccharifying enzyme, and in which: cellulose undergoes primary hydrolysis by adding a saccharifying enzyme thereto, after which the primary hydrolysis product is separated into a primary sugar solution and solid material by solid-liquid separation; water is added to the solid material, and a secondary hydrolysis is carried out, after which the secondary hydrolysis product is separated into a secondary sugar solution and residue by solid-liquid separation; and the primary sugar solution and / or the secondary sugar solution is / are passed through an ultrafiltration membrane, after which the saccharifying enzyme is collected from the film side and the sugar solution is collected from the permeating side. Thus provided is a method for reducing the amount of an enzyme such as cellulase used in a method for manufacturing a sugar solution from a pretreated cellulose material.

Description

technical field [0001] The present invention relates to a method and apparatus for producing sugar liquid from cellulose. Background technique [0002] The chemical fermentation production process using sugar as raw material is used in the production of various industrial raw materials. As the sugar used as the fermentation raw material, sugar derived from edible raw materials such as sugar cane, starch, and sugar beet is currently used industrially, but from the ethical point of view such as the skyrocketing price of edible raw materials due to the increase in the world population in the future, or competition with food, it is possible to construct A process for efficiently producing sugar liquid from recycled non-edible resources, that is, cellulose-containing biomass, or a process for efficiently converting the obtained sugar liquid into an industrial raw material for fermentation will become a future issue. [0003] As a method for producing sugar liquid from cellulose-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12M1/00
CPCC13K1/02C13K13/002C12P19/02C12P19/14Y02E50/10C12M1/12C12N9/2434C12M27/02C12M29/00C12M29/04C12M47/10
Inventor 栗原宏征南野淳山本优树山田胜成
Owner TORAY IND INC