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Method for separating and purifying gamma-polyglutamic acid produced by corn soaking water fermentation

A technology for soaking corn in water and polyglutamic acid is applied in the field of separation engineering to achieve the effects of reducing environmental pollution, shortening extraction time and improving production efficiency

Active Publication Date: 2014-04-02
JILIN COFCO BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the existing γ-polyglutamic acid extraction process, there is no report about the extraction of γ-polyglutamic acid produced by fermentation in the corn soaking process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Separation and purification: use trichloroacetic acid to acidify the fermentation broth of corn soaking water; then use centrifugation to remove bacteria and macromolecular substances; then select an ultrafiltration membrane with a pore size of 0.20 μm, and use it at a pressure of 0.1 MPa and a membrane surface flow rate of Separation was carried out at 4.0 m / s, and the pH value was controlled within the range of 6.0; finally, D318 type weakly basic anion exchange resin was selected as the separation medium, and the pH of the solution was adjusted to 4.0. =3 acetic acid to elute glutamic acid; finally, γ-polyglutamic acid was vacuum-dried to obtain 77.3g γ-polyglutamic acid.

Embodiment 2

[0034] Separation and purification: use trichloroacetic acid to acidify the fermentation broth of corn soaking water; then use centrifugation to remove bacteria and macromolecular substances; then select an ultrafiltration membrane with a pore size of 0.20 μm, and use it at a pressure of 0.15 MPa and a membrane surface flow rate of Separation is carried out at 4.5 m / s, and the pH value is controlled within the range of 6.5; finally, D318 type weakly basic anion exchange resin is selected as the separation medium, and the pH of the solution is adjusted to 4.5. =2 acetic acid to elute glutamic acid; finally, γ-polyglutamic acid was vacuum-dried to obtain 112.8.g γ-polyglutamic acid.

Embodiment 3

[0036] Separation and purification: use trichloroacetic acid to acidify the fermentation broth of corn soaking water; then use centrifugation to remove bacteria and macromolecular substances; then select an ultrafiltration membrane with a pore size of 0.20 μm, and use it at a pressure of 0.2 MPa and a membrane surface flow rate of Separation was carried out at 5.0 m / s, and the pH value was controlled within the range of 7.0; finally, D318 type weakly basic anion exchange resin was selected as the separation medium, and the pH of the solution was adjusted to 5.0, then passed through the moving bed chromatography separation column, and the adsorbed resin was separated by pH =2 acetic acid to elute glutamic acid; finally, γ-polyglutamic acid was vacuum-dried to obtain 155.1 g γ-polyglutamic acid.

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PUM

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Abstract

The invention relates to a method for separating and purifying gamma-polyglutamic acid produced by corn soaking water fermentation, belonging to the technical field of separation engineering. The method comprises the following steps: acidizing, centrifugal separation, filtration with an ultrafiltration membrane, purification with a simulated moving bed, and vacuum drying. The technique is simple, and reduces the environmental pollution; the method extracts gamma-polyglutamic acid from the corn soaking water fermentation liquid with complex components and low gamma-polyglutamic acid content, and shortens the gamma-polyglutamic acid extraction time; and the extraction rate is up to more than 96%. The invention solves the problems of complex separation and purification process, high cost, incapability of continuous operation, low product purity and the like in the existing gamma-polyglutamic acid extraction method.

Description

technical field [0001] The invention belongs to the technical field of separation engineering, and specifically relates to a method for extracting gamma-polyglutamic acid produced by fermentation of corn soaking water. The method involves the extraction and purification of gamma-polyglutamic acid from corn soaking water fermentation liquid by moving bed chromatography acid. Background technique [0002] Corn soaking water (refer to the first edition of "Corn Starch Industry Handbook" in September 2009) contains almost all the soluble nutrients in corn kernels, plus some of the original insoluble substances are hydrolyzed by lactic acid during the soaking process and converted into soluble nutrients. Substances, corn soaking water contains a variety of amino acids, inorganic salts and vitamins. [0003] Qian Dongping and others first separated the protein from the corn soaking water through the isoelectric point method, ultrafiltration method, and trichloroacetic acid method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G69/10
Inventor 佟毅王宏龄金贞花陶进关阳刘跃芹曹雪
Owner JILIN COFCO BIOCHEM