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Construction and detection method of biomolecular motor resonance sensor

A biosensor and molecular motor technology, applied in the new biological field of nano-molecular motor sensor, to shorten the reaction detection time and increase the probability of molecular collision

Inactive Publication Date: 2012-11-28
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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Problems solved by technology

However, the application of resonance phenomena to construct ultrasensitive resonance sensors for biomolecular motors has not been reported.

Method used

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  • Construction and detection method of biomolecular motor resonance sensor
  • Construction and detection method of biomolecular motor resonance sensor
  • Construction and detection method of biomolecular motor resonance sensor

Examples

Experimental program
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preparation example Construction

[0041] 1. Preparation of homogeneous chromophore:

[0042] Inoculate liquid culture medium (see appendix) from thermumicrobium roseum (ATCC 27502) bacteria at a ratio of 1:100, culture at 60°C, 150rpm, for 24h. Then 4000rpm, 30min, 4 ℃ centrifugation to collect bacteria. The cells were resuspended with extraction buffer (20mM Tris-Cl pH8.0, 100mM NaCl, 2mM MgCl2, 1mM DTT), and the supernatant was removed by centrifugation (6000rpm, 10min, 4°C). Add extraction Buffer to resuspend the bacteria (approximately 1 g in 10 ml Buffer), then add a final concentration of 1 mMPMSF, and sonicate for 30 min on ice (5 s of sonication, 8 s off). Centrifuge the broken cells (25,000g, 30min, 4°C), remove the precipitate and get the supernatant. The supernatant was ultracentrifuged (145,000g, 1h, 4°C), and the precipitate was taken as the chromatophore (chromophore), and then the chromatophore with uniform size was obtained through density gradient centrifugation. Finally, resuspend the pell...

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Abstract

The invention provides a construction and detection method of a biomolecular motor resonance sensor. According to the invention, in the process of constructing rotary biological sensors, the phenomenon that activation / suppression exists between load and rotation speed is found and nonlinear relationship exists between signal change and load. The nonlinear phenomenon between rotation and load cannot be explained by common theories of mechanics, but can be described by the resonance theory. According to the invention, motor rotary type sensors are developed into resonance type sensors. The regulation and control factors of the sensors of such type are more complicated and the regulation and control requirements are higher; but once resonance conditions are found to develop the application of the sensors to a higher level, larger principle and technology breakthroughs of the biological sensors are obtained; and after the products are put into use, direct economic benefit and huge social benefit can be generated.

Description

technical field [0001] The invention belongs to the field of nano molecular motor sensor biological technology, discovers the correlation between a rotary ATP motor and resonance, and relates to the design, assembly and application of the resonance sensor of the ATP motor sensor in the biological field. Background technique [0002] Resonance is a technical term in physics. One of the important conditions for resonance is elasticity, and when an object is affected by an external frequency, its frequency must be the same or basically similar to that of the latter. Resonance is not only used in physics at very high frequencies. Moreover, resonance is a universal law of the movement of all matter in the universe, and of course resonance also exists universally in life. However, the application of resonance phenomenon to construct ultrasensitive resonance sensors of biomolecular motors has not been reported. Contents of the invention [0003] In the process of constructing t...

Claims

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Application Information

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IPC IPC(8): G01N33/566G01N33/569G01N33/68C12Q1/70C12Q1/68C12Q1/04C12Q1/44
Inventor 乐加昌王佩荣张旭
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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