Determination of exosomel biomarkers for predicting cardiovascular events

A biomarker, cardiovascular technology, applied in the field of predicting subjects' risk of developing cardiovascular events, risk prognosis of cardiovascular events, kits and biomarkers

Inactive Publication Date: 2012-12-05
卡瓦迪斯有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, plaque material can only be obtained through invasive procedures

Method used

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  • Determination of exosomel biomarkers for predicting cardiovascular events
  • Determination of exosomel biomarkers for predicting cardiovascular events
  • Determination of exosomel biomarkers for predicting cardiovascular events

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Tracking Quantitative Proteomics of Human Plasma Exosomes

[0039] Study population and design

[0040] Athero-Express is a longitudinal vascular biobank study that includes biomaterials from patients who underwent carotid endarterectomy and femoral endarterectomy at two Dutch hospitals (UMC Utrecht and St.Antonius Hospital Nieuwegein) . Approximately 2000 patients have been included to date. Plasma and tissue samples were obtained from all patients before or during endarterectomy (blood draw).

[0041] One year after surgery, all patients were followed up clinically, and mailed questionnaires were filled out at 1, 2, and 3 years after surgery. Physicians were contacted by telephone when patients did not respond to the questionnaire. Endpoint events were adjudicated by an independent endpoint event committee blinded to laboratory results. All endpoints were independently assessed by two members of the committee. In case of disagreement, seek a third opinion.

[0...

Embodiment 2

[0078] Validation of selected proteins in a proof-of-concept study of individual Athero-Express patient blood samples

[0079] Research objectives

[0080] The goal of this study was to identify in individual patient blood samples which of these 17 biomarkers were differentially expressed between patients experiencing secondary coronary events and healthy controls.

[0081] Research design

[0082] Patients in this study underwent carotid surgery due to a primary cerebrovascular event (ie, stroke or transient ischemic attack (TIA)) and were followed for three years. The 17 markers were measured in blood samples from patients experiencing secondary coronary events (29 samples) and age- and sex-matched controls (30 samples). Secondary coronary events were defined as myocardial infarction (fatal and nonfatal), cardiovascular death, sudden death, coronary angioplasty, and coronary artery bypass grafting (CABG).

[0083] Materials and methods

[0084] Exosomes were isolated fro...

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Abstract

The present invention relates to a method of predicting the risk of a subject developing a cardiovascular event, comprising determining the presence of a biomarker that is indicative of the risk of developing a cardiovascular event in exosomes from the subject. The exosomes are suitably isolated from a body fluid selected from serum, plasma, blood, urine, amniotic fluid, malignant ascites, bronchoalveolar lavage fluid, synovial fluid, breast milk, saliva, in particular serum. The biomarker is selected from the proteins Vitronectin, Serpin F2, CD14, Cystatin C, Plasminogen, Nidogen 2 or any combination of two or more of these proteins.

Description

technical field [0001] The present invention relates to the field of risk stratification (grading) and / or patient stratification (grading), and more particularly to the risk prognosis of cardiovascular events such as stroke, transient ischemic attack (TIA), Myocardial infarction (heart attack), brain hemorrhage, and other major abnormalities that occur in blood vessels. [0002] In particular, the present invention relates to methods of predicting a subject's risk of developing a cardiovascular event and kits and biomarkers for use in such methods. Background technique [0003] Established cardiovascular risk factors including dyslipidemia, smoking, hypertension, and diabetes have been incorporated into algorithms for cardiovascular risk assessment. However, identification of patients at risk of developing cardiovascular disease remains difficult. [0004] Identification of prognostic biomarkers will have major added value in identifying patients who are at risk of experie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N2800/32G01N2800/50G01N2333/8139G01N33/6893G01N2333/8121G01N2333/78
Inventor 莱昂纳德斯·蒂默尔斯司肖宽多米尼克斯·帕夏利斯·维克托·德·克莱杰恩
Owner 卡瓦迪斯有限责任公司
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