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Method for extracting secoisolariciresinol diglucoside

A technology of flax lignans and extraction methods, which is applied in the field of extraction of flax lignans, can solve the problems of long extraction time, large solvent consumption, and low extraction volume, and achieve good appearance and color, high relative purity, and biological activity. high effect

Inactive Publication Date: 2012-12-12
上海红马饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the disadvantages of the traditional method for extracting flax lignans, such as long extraction time, large solvent consumption, low extraction amount and high cost, thereby providing a new extraction method of flax lignans , improving the traditional alcohol extraction method, increasing the extraction yield of flax lignans, reducing the amount of solvent used, effectively avoiding resource waste, and laying a foundation for expanding the industrial application value of flax lignans

Method used

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  • Method for extracting secoisolariciresinol diglucoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Weigh 100g of flax cake, add 5g of cellulase, add 500ml of distilled water, stir evenly, adjust pH=4.0, and pretreat for 20min in a water bath at 50°C;

[0031] (2) Adjust the pH to 7.0, add 1167ml of absolute ethanol to make the final concentration 70%, continue stirring and extracting for 30 minutes, ultrasonically assist treatment for 20 minutes, then continue stirring and extracting, after 3 hours in a 50°C water bath, centrifuge at 1500rmp for 10 minutes , take the supernatant.

[0032] (3) Add NaOH solution to the supernatant to adjust the pH=11.5, stir and hydrolyze for 1.5h, keep the pH=11.5 unchanged during this process, centrifuge at 3000rmp for 10min, neutralize the obtained supernatant with HCl solution, adjust the pH to 4.0, Obtain the flax lignans crude extract;

[0033] (4) The crude extract obtained in the previous step is adsorbed through AB-8 type macroporous resin chromatography, and the flow velocity is controlled at 0.5cm / min;

[0034] (5) elu...

Embodiment 2

[0036] (1) Weigh 100g of flax cake, add 5g of cellulase, add 500ml of distilled water, stir evenly, adjust pH=4.2, and pretreat for 30min in a water bath at 55°C;

[0037] (2) Adjust the pH to 7.0, add 1167ml of absolute ethanol to make the final concentration 70%, continue stirring and extracting for 30 minutes, ultrasonically assist treatment for 20 minutes, then continue stirring and extracting, after 3.5 hours in a 55°C water bath, centrifuge at 1500rmp for 5 minutes , take the supernatant.

[0038] (3) Add NaOH solution to the supernatant to adjust pH=12, stir and hydrolyze for 2h, keep pH=12 in the process, centrifuge at 3000rmp for 10min, neutralize the obtained supernatant with HCl solution, and adjust the pH to 4.2 to obtain Flax lignans crude extract;

[0039] (4) The crude extract obtained in the previous step is subjected to AB-8 macroporous resin chromatography adsorption, and the flow rate is controlled at 0.5cm / mim

[0040] (5) Elution with 20% ethanol solutio...

Embodiment 3

[0042] (1) Weigh 100g of flax cake, add 5g of cellulase, add 500ml of distilled water, stir evenly, adjust pH=4.5, and pretreat for 40min in a water bath at 60°C;

[0043](2) Adjust the pH to 7.0, add 1167ml of absolute ethanol to make the final concentration 70%, continue stirring and extracting for 30 minutes, ultrasonically assist treatment for 20 minutes, then continue stirring and extracting, after 4 hours in a water bath at 60°C, centrifuge at 1500rmp for 10 minutes , take the supernatant.

[0044] (3) Add NaOH solution to the supernatant to adjust the pH=12.5, stir and hydrolyze for 2.5h, keep the pH=12.5 constant during this process, centrifuge at 3000rmp for 15min, neutralize the obtained supernatant with HCl solution, adjust the pH to 4.5, Obtain the flax lignans crude extract;

[0045] (4) The crude extract obtained in the previous step is adsorbed through AB-8 type macroporous resin chromatography, and the flow velocity is controlled at 0.5cm / min;

[0046] (5) El...

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Abstract

The invention discloses a method for extracting secoisolariciresinol diglucoside, and the method comprises the following steps of: (1) carrying out hydrolysis pretreatment on a flaxseed cake by cellulose, adding an ethanol solution into the treated flaxseed cake, treating in an ultrasonic-assisted way, stirring and carrying out extraction and centrifugation to obtain a secoisolariciresinol diglucoside crude product; (2) adjusting the pH value of the secoisolariciresinol diglucoside crude product to be 11.5-12.5, stirring and extracting at 50-55 DEG C, removing precipitation by centrifugation to obtain supernate, and adjusting the pH value to be 4.0-4.5; (3) enabling the supernate to be absorbed by macroporous resin chromatography at room temperature; and (4) eluting by using an ethanol solution to obtain an alcoholic solution containing secoisolariciresinol diglucoside, and carrying out vacuum freeze drying to obtain the secoisolariciresinol diglucoside finished product. The invention also discloses secoisolariciresinol diglucoside prepared by the method. The method combines multiple extraction methods, and is low in requirement for production equipment, high in extraction efficiency and obviously superior to the traditional solvent extraction method.

Description

technical field [0001] The invention relates to a method for extracting flax lignans, belonging to the field of development and application of biologically active substances, in particular to a method for extracting, separating and purifying flax lignans from flax cake. Background technique [0002] Flaxseed has become the seventh largest oil crop in the world and the fourth largest oil crop in my country. The main flax planting regions and countries include Canada, India, China, the United States, Argentina, the Nordic region and some Commonwealth of Independent States countries, of which Canada's flaxseed production Highest. The main production areas of flax in China are mainly distributed in North China and Northwest China. Gansu, Inner Mongolia, Ningxia, Hebei and other provinces have the highest flaxseed production, accounting for about 75% of the total national production, followed by Xinjiang, Jilin, Shaanxi and Qinghai, and Southwest China. There are sporadic plantin...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08C07C43/23C07C41/34
Inventor 赵肖刘国庆胡卫华钱晓勇
Owner 上海红马饲料有限公司
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