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Identification method of resistance to aspergillus flavus infection of peanuts and application thereof

An identification method, the technology of Aspergillus flavus, which is applied in the agricultural field, can solve the problems of complex operation, high equipment requirements, and high sample testing costs, and achieve the effects of simple operation, high accuracy of experimental results, and low cost of resistance identification

Inactive Publication Date: 2012-12-12
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method for identifying the resistance of peanuts to Aspergillus flavus infection is mainly to detect the content of Aspergillus flavus after artificial inoculation. The detection methods are thin layer chromatography and high performance liquid chromatography. The operation is complicated, the cost of sample detection is high, and the High equipment requirements cannot be effectively applied to resistance identification in peanut breeding research
It is also possible to estimate the number and degree of Aspergillus flavus infestation by visual observation after manual inoculation. Although the operation is simple, due to the large difference in visual observation, the result error is large, and the resistance of peanuts to Aspergillus flavus infection cannot be effectively evaluated.

Method used

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  • Identification method of resistance to aspergillus flavus infection of peanuts and application thereof
  • Identification method of resistance to aspergillus flavus infection of peanuts and application thereof
  • Identification method of resistance to aspergillus flavus infection of peanuts and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) Use the highly infectious Aspergillus flavus strain As33.2890 (Institute of Microbiology, Chinese Academy of Sciences) as the strain for inoculation; place the Aspergillus flavus strain As33.2890 in Chapei medium and culture it in the dark at 28°C, and use Collect the spores cultured for 7 days in sterile water, resuspend them in sterile water, and adjust the concentration of spores so that each ml of water contains 1×10 8 1 spore, standby as the Aspergillus flavus spore suspension;

[0029] (2) Select the seeds of peanut variety Taishan Sanlirou (Crop Research Institute of Guangdong Academy of Agricultural Sciences), choose healthy peanut seeds, peel off the peel, select seeds with intact seed coats as samples, and pack them into 3 numbered petri dishes In each petri dish, 40 seeds were sterilized with captan and washed with distilled water to make the water content of the seeds 20%, and weighed respectively. The weight of the sample seeds in the petri dish was rec...

Embodiment 2

[0042] (1) Use the highly infectious Aspergillus flavus strain As33.2890 (Institute of Microbiology, Chinese Academy of Sciences) as the strain for inoculation; place the Aspergillus flavus strain As33.2890 in Chapei medium and culture it in the dark at 28°C, and use Collect the spores cultured for 7 days in sterile water, resuspend them in sterile water, and adjust the concentration of spores so that each ml of water contains 1×10 8 1 spore, standby as the Aspergillus flavus spore suspension;

[0043] (2) Select the seeds of peanut variety Zhanqiu 48 (Crop Research Institute of Guangdong Academy of Agricultural Sciences), choose healthy peanut seeds, peel off the peel, select seeds with intact seed coats as samples, and pack them into 3 numbered petri dishes , 30 seeds per petri dish, sterilize the peanut seeds with captan and rinse them with distilled water, so that the water content of the seeds is 20%, weigh them respectively, and record the weight of the sample seeds in t...

Embodiment 3

[0056] (1) Use the highly infectious Aspergillus flavus strain As33.2890 (Institute of Microbiology, Chinese Academy of Sciences) as the strain for inoculation; place the Aspergillus flavus strain As33.2890 in Chapei medium and culture it in the dark at 28°C, and use Collect the spores cultured for 7 days in sterile water, resuspend them in sterile water, and adjust the concentration of spores so that each ml of water contains 1×10 8 1 spore, standby as the Aspergillus flavus spore suspension;

[0057] (2) Select the seeds of peanut variety Yueyou 116 (Crop Research Institute of Guangdong Academy of Agricultural Sciences), choose healthy peanut seeds, peel off the peel, select seeds with intact seed coats as samples, and pack them into 3 numbered petri dishes , 50 seeds per petri dish, sterilize the peanut seeds with captan and rinse them with distilled water, so that the water content of the seeds is 20%, weigh them respectively, and record the weight of the sample seeds in t...

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Abstract

The invention discloses an identification method of resistance to aspergillus flavus infection of peanuts and an application thereof. The identification method disclosed by the invention comprises the following steps: inoculating an aspergillus flavus strain into peanut seeds, further performing dark culture, then adopting a measuring instrument, namely an analytical balance, weighing the seeds after dark culture, finally calculating an aspergillus flavus infection index and performing resistance evaluation. According to the identification method disclosed by the invention, the resistance to the aspergillus flavus infection of a peanut variety can be effectively identified under relatively simple experimental conditions, and the identification method is particularly suitable for batch screening of peanut breeding materials, and has the advantages of simple operation, low resistance identification cost, accurate experimental results and high precision.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for identifying the resistance of peanuts to Aspergillus flavus infection and an application thereof. Background technique [0002] The resistance of peanuts to Aspergillus flavus infection refers to the ability of peanut kernels to inhibit the production of Aspergillus flavus by the above-mentioned fungi after being infected by Aspergillus flavus and A.parasiticus, which have the ability to produce aflatoxin . Peanut varieties that are resistant to A. flavus infection can significantly reduce the level of A. flavus contamination in peanut products. At present, the method for identifying the resistance of peanuts to Aspergillus flavus infection is mainly to detect the content of Aspergillus flavus after artificial inoculation. The detection methods are thin layer chromatography and high performance liquid chromatography. The operation is complicated, the...

Claims

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Application Information

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IPC IPC(8): G01N5/00
Inventor 周桂元梁炫强
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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