Preparation method of nucleic acid adsorption material for separation, purification and recovery of DNA and RNA
An adsorption material, separation and purification technology, which is applied in the field of preparation of nucleic acid adsorption materials for DNA and RNA separation, purification and recovery, can solve the problems of long time consumption, cumbersome operation, low purity and yield, etc., and achieves high yield, high purity, Simple and fast operation method
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Embodiment 1
[0016] see figure 1 , the operation steps of this embodiment are:
[0017] 1) Take high-quality paper fiber: 10%, silicon powder: 15%, and carboxymethyl cellulose: 15% by weight in a triangular flask, and make up to a total volume of 500ml with deionized water.
[0018] 2) Put the triangular flask into a water bath and heat until the above mixture is completely dissolved.
[0019] 3) Homogenize with an electric mixer.
[0020] 4) Pour the homogenate into a six-hole mold with a diameter of 4 cm, place the mold in a 60°C oven and bake for 30 minutes or dry naturally at room temperature to obtain the nucleic acid adsorption material.
[0021] 5) Make the prepared nucleic acid adsorption material into a circular disc with a diameter of 0.7 cm (such as figure 2 shown).
Embodiment 2
[0023] see figure 1 , the operation steps of this embodiment are:
[0024] 1) Take high-quality paper fiber: 5%, silicon powder: 10%, carboxymethyl cellulose: 10% by weight in a triangular flask, and make up to a total volume of 500ml with deionized water.
[0025] 2) Put the triangular flask into a water bath and heat until the above mixture is completely dissolved.
[0026] 3) Homogenize with an electric mixer.
[0027] 4) Pour the homogenate into a six-hole mold with a diameter of 4 cm, place the mold in a 60°C oven and bake for 30 minutes or dry naturally at room temperature to obtain the nucleic acid adsorption material.
[0028] 5) Make the prepared adsorbent material into a circular paper sheet with a diameter of 0.7cm (such as figure 2 shown).
experiment example 1
[0029] Experimental Example 1: Rapid separation of trace amounts of blood RNA nucleic acid adsorption
[0030] The nucleic acid adsorption material prepared in the above embodiment is put into the inner layer of the nucleic acid adsorption centrifuge sleeve as the nucleic acid adsorption medium, and the outer sleeve is a liquid collection tube, and the inner adsorption tube is inserted into the outer collection tube during use.
[0031] The RNA extraction reagent consists of erythrocyte lysis solution, dissociation solution, clearing solution, washing solution and separation solution.
[0032] The components and substance concentration (mol / liter) of the RNA extraction reagent are composed according to the final concentration and volume:
[0033] Red blood cell lysis solution: 0.01-1mol / L NH 4 Cl, 0.0001-0.3mol / L KHCO 3 , the balance of 0.00001-1mol / L EDTA is deionized water;
[0034] Dissociation solution: 0.1-10mol / L guanidine salt, 0.005-0.5mol / L C 6 h 5 Na 3 o 7 , 0...
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