Preparation method of Astragalus mongholicus-toad oral colon targeting tablets for treating colorectal carcinoma
A colon targeting and colon cancer technology, which can be used in medical preparations containing active ingredients, pharmaceutical formulations, and medical raw materials derived from amphibians, etc., and can solve the problems of limited varieties of Chinese medicines and Chinese medicine compound preparations on the market.
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Embodiment 1
[0036] Embodiment 1: the preparation of mixed extract
[0037] Radix Astragali 15g, add 6 times of 80% ethanol and carry out reflux extraction, extract 3 times, each time 1.5 hours, combine ethanol extract to obtain extract A, extract A recovers ethanol to have no alcohol taste, concentrates to the solid-liquid ratio is 1: 0.5, to obtain the concentrated solution of astragalus alcohol extraction;
[0038] Add 10 times of water to 12g of raw rehmannia and astragalus dregs, decoct 3 times, 1.5 hours each time, combine the water extracts to obtain extract B, add ethanol to the extract B after concentration, so that the alcohol content reaches 50%, and let it stand After 24 hours, filter with suction to obtain crude polysaccharide Ⅰ. After wiping off ethanol, add water until the ratio of medicine to liquid is 1:1, then add ethanol to make the alcohol content reach 50%. After standing for 24 hours, filter with suction to obtain crude polysaccharide II;
[0039] Dried toad skin 9g...
Embodiment 2
[0044] Embodiment 2: the preparation of mixed extract
[0045] Astragalus 12g plus 10 times 90% ethanol for reflux extraction, extraction 2 times, 1 hour each time, combined ethanol extract to obtain extract A, extract A was recovered from ethanol until it had no alcohol smell, and concentrated until the ratio of solid to liquid was 1:1 , to obtain the concentrated solution of astragalus alcohol extraction;
[0046] Add 8 times of water to 6g of raw land and astragalus dregs, decoct twice for 2 hours each time, combine the water extracts to obtain extract B, add ethanol to the extract B after concentration, so that the alcohol content reaches 60%, and let it stand After 48 hours, filter with suction to obtain crude polysaccharide Ⅰ. After wiping off ethanol, add water to a liquid ratio of 1:0.5, then add ethanol to make the alcohol content reach 60%. After standing still for 48 hours, filter with suction to obtain crude polysaccharide II;
[0047] Add 10 times 80% ethanol to...
Embodiment 3
[0052] Embodiment 3: the preparation of mixed extract
[0053] Astragalus membranaceus 30g plus 8 times 70% ethanol for reflux extraction, extraction 2 times, 1.5 hours each time, combined ethanol extract to obtain extract A, extract A was recovered from ethanol until it had no alcohol smell, and concentrated to a solid-liquid ratio of 1:0.8 , to obtain the concentrated solution of astragalus alcohol extraction;
[0054] Add 6 times of water to 15g of raw land and astragalus dregs, decoct 3 times, 2 hours each time, combine the water extracts to obtain extract B, add ethanol to the extract B after concentration, so that the alcohol content reaches 70%, and let it stand After 24 hours, filter with suction to obtain crude polysaccharide Ⅰ. After wiping off ethanol, add water until the ratio of medicine to liquid is 1:1, then add ethanol to make the alcohol content reach 70%. After standing for 24 hours, filter with suction to obtain crude polysaccharide II;
[0055] Add 3g of ...
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