The invention discloses a yao medicine composition for preventing and treating hyperplasia of mammary glands. The yao medicine composition is prepared from, by weight, 1-30 parts of puberulous glochidion herb, 5-60 parts of stauntonia obovatifoliola, 1-30 parts of all-grass of Japanese violet, 1-30 parts of herba sarcandrae, 1-30 parts of radix tinosporae, 5-60 parts of false-yellowflower milkwort root or herb, 1-20 parts of radix paeoniae alba, 1-20 parts of radix bupleuri, 1-20 parts of spina date seeds, 1-20 parts of spica prunellae, 1-20 parts of radix angelica sinensis and 1-30 parts of suberect spatholobus stems. The invention further discloses a preparing method of the yao medicine composition. Herba sarcandrae and radix tinosporae are strong in nature, thus serving as beating medicine; false-yellowflower milkwort root or herb and suberect spatholobus stems are mild in nature, thus serving as wind medicine; puberulous glochidion herb is mild in nature and serves as both wind medicine and beating medicine; the whole formula is mainly prepared from beating medicine, both wind medicine and beating medicine exist, pathogen elimination can be achieved without hurting body resistance, and the ideas of yao medicine that wind medicine is for nourishment and beating medicine is for evil elimination and redundancy and deficiency are balanced are fully reflected; the yao medicine composition can promote blood circulation to remove blood stasis and resolve hard lump, and has an obvious curative effect when used for preventing and treating hyperplasia of mammary glands.