Restoration method for degrading soil PCP residue through cooperation of earthworms and microbes

A technology of microbial degradation and repair method, which is applied in the repair field of earthworms and microorganisms to degrade soil PCP residues, can solve PCP residues and other problems, and achieve the effects of accelerated decomposition, accelerated elimination and detoxification, and simple and effective repair

Inactive Publication Date: 2013-01-02
SOUTH CHINA AGRI UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a repair method for earthworms to cooperate with microorganisms to deg

Method used

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  • Restoration method for degrading soil PCP residue through cooperation of earthworms and microbes
  • Restoration method for degrading soil PCP residue through cooperation of earthworms and microbes
  • Restoration method for degrading soil PCP residue through cooperation of earthworms and microbes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Tolerance to soil PCP of P.

[0033] Experimental design The number of Eisenia chinensis was 15, and the number of Esperus grandiosa was 7. Experiments were carried out respectively. Different amounts of PCP were added to the soil without PCP, and the PCP concentration was prepared to be 0 mg·kg -1 (A), 20 mg?kg -1 (B), 40 mg?kg -1 (C), 60 mg?kg -1 (D), 80 mg?kg -1 (E), 100 mg?kg -1 (F), 150 mg?kg - 1 (G) and 200 mg·kg -1 (H) Experimental soil, replicated three times for a total of 24 treatments. The weight of the experimental soil was 0.5 kg. The soil moisture in each experiment was always maintained at 60% of the saturated moisture content. Put the experimental soil into the bucket, then put the earthworms in, wrap the mouth of the bucket with tin foil, and pierce several small holes with pins to keep the air circulation in the bucket. Cultivate under shaded conditions. The experimental design measures the biomass of two ecological types of earthworms un...

Embodiment 2

[0059] Example 2 Degradation PCP ability identification of microbial strains

[0060] Klebsiella were inoculated into enriched liquid medium for enrichment growth (150 r?min -1 Aerobic shaker culture, 30°C) for 24 h, and each strain setting was repeated three times. Take 10mL of the strain culture solution at 6000 r?min -1 Ultracentrifuge for 10 min, remove the supernatant, rinse with sterilized deionized water, and then centrifuge again, repeat this three times; finally resuspend the cleaned cells with sterilized deionized water. The concentration of the bacterial suspension was measured by the absorbance value at λ = 600 nm. Finally, take 10 mL of bacterial liquid and add it to 90 mL of inorganic salt culture solution, and add PCP at a concentration of 100 mg?L -1 , PCP concentrations in different treatments were measured at 0, 24, 48, 96, and 144 h; the PCP-containing inorganic salt culture solution without adding bacterial solution was used as the control.

[0061] T...

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Abstract

The invention discloses a restoration method for degrading soil PCP residue through the cooperation of earthworms and microbes. The invention belongs to the technical field of soil pollutant restoration. According to the restoration method provided by the invention, eisenia foetida and/or amynthas robustus are placed in polluted soil, and fermented chicken dung and/or cow dung is added simultaneously; the fermented chicken dung and/or cow dung is sufficiently mixed with soil; and fermented chicken dung and/or cow dung is added again every three months. With the restoration method provided by the invention, soil PCP elimination and detoxification can be accelerated, various soil physicochemical properties can be improved through the movement of the earthworms, soil organic matter decomposition can be accelerated, reaction conditions of detoxification of other pollutants can be optimized, and form transformation and degradation reactions of other pollutants contained in soil can be biologically catalyzed.

Description

technical field [0001] The invention relates to the technical field of contaminated soil remediation, in particular to a remediation method in which earthworms cooperate with microorganisms to degrade soil PCP residues. Background technique [0002] PCP (pentachlorophenol, pentachlorophenol) is widely used as a herbicide, fungicide and wood preservative. It has the characteristics of "three causes" of teratogenicity, carcinogenicity and mutagenicity. It has been listed as an environmental priority by the US Environmental Protection Agency and other agencies. List of controlled pollutants. Although its production and use have been banned worldwide since the 1980s, it is still widely detected in soil, water, food and animal samples due to its stable chemical properties and not easy to degrade. PCP pollution has seriously threatened the environment of agricultural production areas and the quality and safety of agricultural products, which in turn affects human health. [0003...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
Inventor 李永涛蔺中蔡燕飞李晓敏孙礼勇陈昊
Owner SOUTH CHINA AGRI UNIV
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